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Increased peritoneal and endometrial gene expression of biologically relevant cytokines and growth factors during the menstrual phase in women with endometriosis.
Fertil Steril. 2006 Jun; 85(6):1667-75.FS

Abstract

OBJECTIVE

To examine differential messenger RNA (mRNA) expression of relevant cytokines, metalloproteases, growth and adhesion factors in endometrium and peritoneum from women with endometriosis when compared with women without the disease during menstrual and luteal phases of the cycle.

DESIGN

Patients with endometriosis were compared with control patients.

SETTING

University hospital.

PATIENT(S)

A total of 35 patients (20 patients during the luteal phase and 15 patients during the menstrual phase) were selected for this study on the basis of cycle phase and presence or absence of endometriosis.

INTERVENTION(S)

In this study, endometriosis was laparoscopically and histologically confirmed in 24 women with endometriosis of revised American Society for Reproductive Medicine (ASRM) stage I-II (n = 12) and revised ASRM stage III-IV (n = 12), and the presence of a normal pelvis was documented by laparoscopy in 11 control patients. The macroscopically normal peritoneum tissues were collected from lateral wall left or right, near the colon ascendens or descendens.

MAIN OUTCOME MEASURE(S)

The expression levels were determined as ratios between the target molecules and beta-actin as housekeeping gene.

RESULT(S)

In women with endometriosis, peritoneal mRNA levels of matrix metalloproteinase (MMP)-3, transforming growth factor-beta, interleukin (IL)-6, and intercellular adhesion molecule-1 and endometrial mRNA levels of MMP-3, tumor necrosis factor (TNF)-alpha, and IL-8 were significantly higher during the menstrual phase when compared with luteal phase. During the menstrual phase of the cycle, both endometrial expression of TNF-alpha, IL-8, and MMP-3 mRNA levels and peritoneal expression of transforming growth factor-beta, IL-6, and intercellular adhesion molecule-1 mRNA levels were significantly higher in women with endometriosis when compared with controls. Immunohistochemical staining confirmed the presence of TNF-alpha in peritoneum and endometrium in both women with endometriosis and controls.

CONCLUSION(S)

Increased endometrial and peritoneal cytokine mRNA expression during menstruation may contribute to a pelvic inflammatory microenvironment favoring the development of endometriosis.

Authors+Show Affiliations

Leuven University Fertility Centre, Department of Obstetrics and Gynaecology, University Hospital Gasthuisberg, Leuven, Belgium.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Controlled Clinical Trial
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16759923

Citation

Kyama, Cleophas M., et al. "Increased Peritoneal and Endometrial Gene Expression of Biologically Relevant Cytokines and Growth Factors During the Menstrual Phase in Women With Endometriosis." Fertility and Sterility, vol. 85, no. 6, 2006, pp. 1667-75.
Kyama CM, Overbergh L, Debrock S, et al. Increased peritoneal and endometrial gene expression of biologically relevant cytokines and growth factors during the menstrual phase in women with endometriosis. Fertil Steril. 2006;85(6):1667-75.
Kyama, C. M., Overbergh, L., Debrock, S., Valckx, D., Vander Perre, S., Meuleman, C., Mihalyi, A., Mwenda, J. M., Mathieu, C., & D'Hooghe, T. M. (2006). Increased peritoneal and endometrial gene expression of biologically relevant cytokines and growth factors during the menstrual phase in women with endometriosis. Fertility and Sterility, 85(6), 1667-75.
Kyama CM, et al. Increased Peritoneal and Endometrial Gene Expression of Biologically Relevant Cytokines and Growth Factors During the Menstrual Phase in Women With Endometriosis. Fertil Steril. 2006;85(6):1667-75. PubMed PMID: 16759923.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increased peritoneal and endometrial gene expression of biologically relevant cytokines and growth factors during the menstrual phase in women with endometriosis. AU - Kyama,Cleophas M, AU - Overbergh,Lutgart, AU - Debrock,Sophie, AU - Valckx,Dirk, AU - Vander Perre,Sarah, AU - Meuleman,Christel, AU - Mihalyi,Attila, AU - Mwenda,Jason M, AU - Mathieu,Chantal, AU - D'Hooghe,Thomas M, PY - 2005/06/01/received PY - 2005/11/18/revised PY - 2005/11/18/accepted PY - 2006/6/9/pubmed PY - 2006/6/28/medline PY - 2006/6/9/entrez SP - 1667 EP - 75 JF - Fertility and sterility JO - Fertil. Steril. VL - 85 IS - 6 N2 - OBJECTIVE: To examine differential messenger RNA (mRNA) expression of relevant cytokines, metalloproteases, growth and adhesion factors in endometrium and peritoneum from women with endometriosis when compared with women without the disease during menstrual and luteal phases of the cycle. DESIGN: Patients with endometriosis were compared with control patients. SETTING: University hospital. PATIENT(S): A total of 35 patients (20 patients during the luteal phase and 15 patients during the menstrual phase) were selected for this study on the basis of cycle phase and presence or absence of endometriosis. INTERVENTION(S): In this study, endometriosis was laparoscopically and histologically confirmed in 24 women with endometriosis of revised American Society for Reproductive Medicine (ASRM) stage I-II (n = 12) and revised ASRM stage III-IV (n = 12), and the presence of a normal pelvis was documented by laparoscopy in 11 control patients. The macroscopically normal peritoneum tissues were collected from lateral wall left or right, near the colon ascendens or descendens. MAIN OUTCOME MEASURE(S): The expression levels were determined as ratios between the target molecules and beta-actin as housekeeping gene. RESULT(S): In women with endometriosis, peritoneal mRNA levels of matrix metalloproteinase (MMP)-3, transforming growth factor-beta, interleukin (IL)-6, and intercellular adhesion molecule-1 and endometrial mRNA levels of MMP-3, tumor necrosis factor (TNF)-alpha, and IL-8 were significantly higher during the menstrual phase when compared with luteal phase. During the menstrual phase of the cycle, both endometrial expression of TNF-alpha, IL-8, and MMP-3 mRNA levels and peritoneal expression of transforming growth factor-beta, IL-6, and intercellular adhesion molecule-1 mRNA levels were significantly higher in women with endometriosis when compared with controls. Immunohistochemical staining confirmed the presence of TNF-alpha in peritoneum and endometrium in both women with endometriosis and controls. CONCLUSION(S): Increased endometrial and peritoneal cytokine mRNA expression during menstruation may contribute to a pelvic inflammatory microenvironment favoring the development of endometriosis. SN - 1556-5653 UR - https://www.unboundmedicine.com/medline/citation/16759923/Increased_peritoneal_and_endometrial_gene_expression_of_biologically_relevant_cytokines_and_growth_factors_during_the_menstrual_phase_in_women_with_endometriosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0015-0282(06)00492-4 DB - PRIME DP - Unbound Medicine ER -