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[In vivo tracing of transferred apoptotic cell labeled using CFSE: a flow cytometry-based assay method].
Nan Fang Yi Ke Da Xue Xue Bao. 2006 May; 26(5):599-602.NF

Abstract

OBJECTIVE

To establish an assay method for detecting the migration of transferred apoptotic cells into the recipient using flow cytometry.

METHODS

Spleen lymphocytes were isolated and labeled with an intracellular amine dye, carboxyfluorescein diacetate succinimidyl ester (CFSE), to allow discrimination. The labeled cells were induced with dexamethasone to undergo apoptosis and transferred into recipient mice via tail venous transfusion. Flow cytometry and histological examination of different tissues were performed at different time points. The stability of CFSE labeling for apoptotic cells was also tested.

RESULTS

The CFSE-labeled apoptotic cells were highly fluorescent with a positive labeling rate of (98.0+/-1.9)%. The stability of CFSE-labeling was testified, and the CFSE-labeled apoptotic cells entering different tissues at different time points were detected by flow cytometry and verified by histological examination.

CONCLUSION

Flow cytometry using CFSE labeling is reliable, sensitive, precise and convenient for apoptotic cell tracing in vivo and in vitro.

Authors+Show Affiliations

Wang Y
Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
Gao Y
No affiliation info available
Sun EW
No affiliation info available
Xie JM
No affiliation info available
Zhang HY
No affiliation info available
Chen JB
No affiliation info available

MeSH

Adoptive TransferAnimalsApoptosisDexamethasoneFemaleFlow CytometryFluoresceinsFluorescent DyesLymphocytesMiceMice, Inbred BALB CReproducibility of ResultsSpleenSuccinimides

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

16762859

Citation

Wang, Yan, et al. "[In Vivo Tracing of Transferred Apoptotic Cell Labeled Using CFSE: a Flow Cytometry-based Assay Method]." Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University, vol. 26, no. 5, 2006, pp. 599-602.
Wang Y, Gao Y, Sun EW, et al. [In vivo tracing of transferred apoptotic cell labeled using CFSE: a flow cytometry-based assay method]. Nan Fang Yi Ke Da Xue Xue Bao. 2006;26(5):599-602.
Wang, Y., Gao, Y., Sun, E. W., Xie, J. M., Zhang, H. Y., & Chen, J. B. (2006). [In vivo tracing of transferred apoptotic cell labeled using CFSE: a flow cytometry-based assay method]. Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University, 26(5), 599-602.
Wang Y, et al. [In Vivo Tracing of Transferred Apoptotic Cell Labeled Using CFSE: a Flow Cytometry-based Assay Method]. Nan Fang Yi Ke Da Xue Xue Bao. 2006;26(5):599-602. PubMed PMID: 16762859.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [In vivo tracing of transferred apoptotic cell labeled using CFSE: a flow cytometry-based assay method]. AU - Wang,Yan, AU - Gao,Yi, AU - Sun,Er-wei, AU - Xie,Jin-min, AU - Zhang,Hui-ying, AU - Chen,Jian-bo, PY - 2006/6/10/pubmed PY - 2008/4/25/medline PY - 2006/6/10/entrez SP - 599 EP - 602 JF - Nan fang yi ke da xue xue bao = Journal of Southern Medical University JO - Nan Fang Yi Ke Da Xue Xue Bao VL - 26 IS - 5 N2 - OBJECTIVE: To establish an assay method for detecting the migration of transferred apoptotic cells into the recipient using flow cytometry. METHODS: Spleen lymphocytes were isolated and labeled with an intracellular amine dye, carboxyfluorescein diacetate succinimidyl ester (CFSE), to allow discrimination. The labeled cells were induced with dexamethasone to undergo apoptosis and transferred into recipient mice via tail venous transfusion. Flow cytometry and histological examination of different tissues were performed at different time points. The stability of CFSE labeling for apoptotic cells was also tested. RESULTS: The CFSE-labeled apoptotic cells were highly fluorescent with a positive labeling rate of (98.0+/-1.9)%. The stability of CFSE-labeling was testified, and the CFSE-labeled apoptotic cells entering different tissues at different time points were detected by flow cytometry and verified by histological examination. CONCLUSION: Flow cytometry using CFSE labeling is reliable, sensitive, precise and convenient for apoptotic cell tracing in vivo and in vitro. SN - 1673-4254 UR - https://www.unboundmedicine.com/medline/citation/16762859/[In_vivo_tracing_of_transferred_apoptotic_cell_labeled_using_CFSE:_a_flow_cytometry_based_assay_method]_ DB - PRIME DP - Unbound Medicine ER -