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Identification of bifunctional delta12/omega3 fatty acid desaturases for improving the ratio of omega3 to omega6 fatty acids in microbes and plants.
Proc Natl Acad Sci U S A. 2006 Jun 20; 103(25):9446-51.PN

Abstract

We report the identification of bifunctional Delta12/omega3 desaturases from Fusarium moniliforme, Fusarium graminearum, and Magnaporthe grisea. The bifunctional activity of these desaturases distinguishes them from all known Delta12 or omega3 fatty acid desaturases. The omega3 desaturase activity of these enzymes also shows a broad omega6 fatty acid substrate specificity by their ability to convert linoleic acid (LA), gamma-linolenic acid, di-homo-gamma-linolenic acid, and arachidonic acid to the omega3 fatty acids, alpha-linolenic acid (ALA), stearidonic acid, eicosatetraenoic acid, and eicosapentaenoic acid (EPA), respectively. Phylogenetic analysis suggests that omega3 desaturases arose by independent gene duplication events from a Delta12 desaturase ancestor. Expression of F. moniliforme Delta12/omega3 desaturase resulted in high ALA content in both Yarrowia lipolytica, an oleaginous yeast naturally deficient in omega3 desaturation, and soybean. In soybean, seed-specific expression resulted in 70.9 weight percent of total fatty acid (%TFA) ALA in a transformed seed compared with 10.9%TFA in a null segregant seed and 53.2%TFA in the current best source of ALA, linseed oil. The ALA/LA ratio in transformed seed was 22.3, a 110- and 7-fold improvement over the null segregant seed and linseed oil, respectively. Thus, these desaturases have potential for producing nutritionally desirable omega3 long-chain polyunsaturated fatty acids, such as EPA, with a significantly improved ratio of omega3/omega6 long-chain polyunsaturated fatty acids in both oilseeds and oleaginous microbes.

Authors+Show Affiliations

Crop Genetics, Pioneer, Dupont Company, Dupont Experimental Station, Wilmington, DE 19880, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16763049

Citation

Damude, Howard G., et al. "Identification of Bifunctional Delta12/omega3 Fatty Acid Desaturases for Improving the Ratio of Omega3 to Omega6 Fatty Acids in Microbes and Plants." Proceedings of the National Academy of Sciences of the United States of America, vol. 103, no. 25, 2006, pp. 9446-51.
Damude HG, Zhang H, Farrall L, et al. Identification of bifunctional delta12/omega3 fatty acid desaturases for improving the ratio of omega3 to omega6 fatty acids in microbes and plants. Proc Natl Acad Sci U S A. 2006;103(25):9446-51.
Damude, H. G., Zhang, H., Farrall, L., Ripp, K. G., Tomb, J. F., Hollerbach, D., & Yadav, N. S. (2006). Identification of bifunctional delta12/omega3 fatty acid desaturases for improving the ratio of omega3 to omega6 fatty acids in microbes and plants. Proceedings of the National Academy of Sciences of the United States of America, 103(25), 9446-51.
Damude HG, et al. Identification of Bifunctional Delta12/omega3 Fatty Acid Desaturases for Improving the Ratio of Omega3 to Omega6 Fatty Acids in Microbes and Plants. Proc Natl Acad Sci U S A. 2006 Jun 20;103(25):9446-51. PubMed PMID: 16763049.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of bifunctional delta12/omega3 fatty acid desaturases for improving the ratio of omega3 to omega6 fatty acids in microbes and plants. AU - Damude,Howard G, AU - Zhang,Hongxiang, AU - Farrall,Leonard, AU - Ripp,Kevin G, AU - Tomb,Jean-Francois, AU - Hollerbach,Dieter, AU - Yadav,Narendra S, Y1 - 2006/06/08/ PY - 2006/6/10/pubmed PY - 2006/8/15/medline PY - 2006/6/10/entrez SP - 9446 EP - 51 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc Natl Acad Sci U S A VL - 103 IS - 25 N2 - We report the identification of bifunctional Delta12/omega3 desaturases from Fusarium moniliforme, Fusarium graminearum, and Magnaporthe grisea. The bifunctional activity of these desaturases distinguishes them from all known Delta12 or omega3 fatty acid desaturases. The omega3 desaturase activity of these enzymes also shows a broad omega6 fatty acid substrate specificity by their ability to convert linoleic acid (LA), gamma-linolenic acid, di-homo-gamma-linolenic acid, and arachidonic acid to the omega3 fatty acids, alpha-linolenic acid (ALA), stearidonic acid, eicosatetraenoic acid, and eicosapentaenoic acid (EPA), respectively. Phylogenetic analysis suggests that omega3 desaturases arose by independent gene duplication events from a Delta12 desaturase ancestor. Expression of F. moniliforme Delta12/omega3 desaturase resulted in high ALA content in both Yarrowia lipolytica, an oleaginous yeast naturally deficient in omega3 desaturation, and soybean. In soybean, seed-specific expression resulted in 70.9 weight percent of total fatty acid (%TFA) ALA in a transformed seed compared with 10.9%TFA in a null segregant seed and 53.2%TFA in the current best source of ALA, linseed oil. The ALA/LA ratio in transformed seed was 22.3, a 110- and 7-fold improvement over the null segregant seed and linseed oil, respectively. Thus, these desaturases have potential for producing nutritionally desirable omega3 long-chain polyunsaturated fatty acids, such as EPA, with a significantly improved ratio of omega3/omega6 long-chain polyunsaturated fatty acids in both oilseeds and oleaginous microbes. SN - 0027-8424 UR - https://www.unboundmedicine.com/medline/citation/16763049/Identification_of_bifunctional_delta12/omega3_fatty_acid_desaturases_for_improving_the_ratio_of_omega3_to_omega6_fatty_acids_in_microbes_and_plants_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=16763049 DB - PRIME DP - Unbound Medicine ER -