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Separation of plant hormones from biofertilizer by capillary electrophoresis using a capillary coated dynamically with polycationic polymers.
Anal Sci. 2006 Jun; 22(6):811-4.AS

Abstract

A new, simple and rapid capillary electrophoresis (CE) method, using hexadimethrine bromide (HDB) as electroosmotic flow (EOF) modifier, was developed for the identification and quantitative determination of four plant hormones, including gibberellin A3 (GA3), indole-3-acetic acid (IAA), alpha-naphthaleneacetic acid (NAA) and 4-chlorophenoxyacetic acid (4-CA). The optimum separation was achieved with 20 mM borate buffer at pH 10.00 containing 0.005% (w/v) of HDB. The applied voltage was -25 kV and the capillary temperature was kept constant at 25 degrees C. Salicylic acid was used as internal standard for quantification. The calibration dependencies exhibited good linearity within the ratios of the concentrations of standard samples and internal standard and the ratios of the peak areas of samples and internal standard. The correlation coefficients were from 0.9952 to 0.9997. The relative standard deviations of migration times and peak areas were < 1.93 and 6.84%, respectively. The effects of buffer pH, the concentration of HDB and the voltage on the resolution were studied systematically. By this method, the contents of plant hormone in biofertilizer were successfully determined within 7 min, with satisfactory repeatability and recovery.

Authors+Show Affiliations

Jiang TF
Marine Drug and Food Institute, Ocean University of China, Qingdao, P. R. China.
Lv ZH
No affiliation info available
Wang YH
No affiliation info available
Yue ME
No affiliation info available

MeSH

2,4-Dichlorophenoxyacetic AcidCalibrationCationsElectrophoresis, CapillaryFertilizersGibberellinsHexadimethrine BromideHydrogen-Ion ConcentrationIndoleacetic AcidsMethanolModels, ChemicalNaphthaleneacetic AcidsPlant Growth RegulatorsPolymersTime Factors

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16772676

Citation

Jiang, Ting-Fu, et al. "Separation of Plant Hormones From Biofertilizer By Capillary Electrophoresis Using a Capillary Coated Dynamically With Polycationic Polymers." Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry, vol. 22, no. 6, 2006, pp. 811-4.
Jiang TF, Lv ZH, Wang YH, et al. Separation of plant hormones from biofertilizer by capillary electrophoresis using a capillary coated dynamically with polycationic polymers. Anal Sci. 2006;22(6):811-4.
Jiang, T. F., Lv, Z. H., Wang, Y. H., & Yue, M. E. (2006). Separation of plant hormones from biofertilizer by capillary electrophoresis using a capillary coated dynamically with polycationic polymers. Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry, 22(6), 811-4.
Jiang TF, et al. Separation of Plant Hormones From Biofertilizer By Capillary Electrophoresis Using a Capillary Coated Dynamically With Polycationic Polymers. Anal Sci. 2006;22(6):811-4. PubMed PMID: 16772676.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Separation of plant hormones from biofertilizer by capillary electrophoresis using a capillary coated dynamically with polycationic polymers. AU - Jiang,Ting-Fu, AU - Lv,Zhi-Hua, AU - Wang,Yuan-Hong, AU - Yue,Mei-E, PY - 2006/6/15/pubmed PY - 2007/5/26/medline PY - 2006/6/15/entrez SP - 811 EP - 4 JF - Analytical sciences : the international journal of the Japan Society for Analytical Chemistry JO - Anal Sci VL - 22 IS - 6 N2 - A new, simple and rapid capillary electrophoresis (CE) method, using hexadimethrine bromide (HDB) as electroosmotic flow (EOF) modifier, was developed for the identification and quantitative determination of four plant hormones, including gibberellin A3 (GA3), indole-3-acetic acid (IAA), alpha-naphthaleneacetic acid (NAA) and 4-chlorophenoxyacetic acid (4-CA). The optimum separation was achieved with 20 mM borate buffer at pH 10.00 containing 0.005% (w/v) of HDB. The applied voltage was -25 kV and the capillary temperature was kept constant at 25 degrees C. Salicylic acid was used as internal standard for quantification. The calibration dependencies exhibited good linearity within the ratios of the concentrations of standard samples and internal standard and the ratios of the peak areas of samples and internal standard. The correlation coefficients were from 0.9952 to 0.9997. The relative standard deviations of migration times and peak areas were < 1.93 and 6.84%, respectively. The effects of buffer pH, the concentration of HDB and the voltage on the resolution were studied systematically. By this method, the contents of plant hormone in biofertilizer were successfully determined within 7 min, with satisfactory repeatability and recovery. SN - 0910-6340 UR - https://www.unboundmedicine.com/medline/citation/16772676/Separation_of_plant_hormones_from_biofertilizer_by_capillary_electrophoresis_using_a_capillary_coated_dynamically_with_polycationic_polymers_ DB - PRIME DP - Unbound Medicine ER -