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Testicular inhibition of tyrosine hydroxylase expression in tuberoinfundibular and nigrostriatal dopaminergic neurons.
Endocrinology. 1991 Aug; 129(2):877-82.E

Abstract

We have investigated, in young and aged rats, tuberoinfundibular dopaminergic (TIDA) and nigrostriatal neurons of the brain, and the role of the testes and anterior pituitary (AP) on 1) the in situ activity of tyrosine hydroxylase (TH), 2) translation of TH mRNA, as reflected by the mass of TH, and 3) transcription of the TH gene, as revealed by the mass of TH mRNA. The median eminence (ME) and corpus striatum (CS) were used as the sources of proximal neurites of the TIDA neurons and nigrostriatal neurons, respectively. The arcuate-periventricular nuclei of the ventral hypothalamus were used as the source of perikarya of the TIDA neurons, and substantia nigra (SN) nuclei of the midbrain were used as the source of perikarya of nigrostriatal neurons. The in situ activity of TH was calculated using the rate of accumulation of dihydroxyphenylalanine after pharmacological inhibition of dihydroxyphenylalanine decarboxylase activity. TH mass and TH mRNA were measured using an immunoblot assay and an S1 nuclease protection assay, respectively. Compared to intact animals, orchidectomized young and aged male rats had significantly (P less than 0.001) increased in situ activity of TH in the ME and CS, but not the SN. Orchidectomy also caused a significant (P less than 0.01) increase in the quantity of TH in the ME and a 2- to 3-fold increase in TH in the CS and SN. In contrast to castration, AP grafts, implanted in the lateral ventricles of the brain, caused a significant (P less than 0.001) increase only in TH activity in the ME. No effect of AP grafts was seen on TH activity of the CS or SN. AP grafts had no effect on the amount of TH in the ME, CS, or SN. In the TIDA neurons of young males and in the nigrostriatal neurons of young and aged castrates, the amount of TH mRNA was not different from that in intact males. AP grafts had no effect on TH mRNA in these dopaminergic neurons. These studies show that TIDA neurons and nigrostriatal neurons share some common aspects in their regulation and are dissimilar in others. AP grafts stimulate TH activity in young as well as aged TIDA neurons, but not in nigrostriatal neurons. Castration leads to an increase in TH mass in both TIDA and nigrostriatal neurons. The increase in TH mass is not associated with an increase in the amount of TH mRNA. It is suggested that castration results in increased translation of TH mRNA, but not transcription of the TH gene.(ABSTRACT TRUNCATED AT 400 WORDS)

Authors+Show Affiliations

Department of Obstetrics and Gynecology, University of Texas Southwestern Medical Center, Dallas 75235-9032.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

1677330

Citation

Aguila-Mansilla, N, et al. "Testicular Inhibition of Tyrosine Hydroxylase Expression in Tuberoinfundibular and Nigrostriatal Dopaminergic Neurons." Endocrinology, vol. 129, no. 2, 1991, pp. 877-82.
Aguila-Mansilla N, Kedzierski W, Porter JC. Testicular inhibition of tyrosine hydroxylase expression in tuberoinfundibular and nigrostriatal dopaminergic neurons. Endocrinology. 1991;129(2):877-82.
Aguila-Mansilla, N., Kedzierski, W., & Porter, J. C. (1991). Testicular inhibition of tyrosine hydroxylase expression in tuberoinfundibular and nigrostriatal dopaminergic neurons. Endocrinology, 129(2), 877-82.
Aguila-Mansilla N, Kedzierski W, Porter JC. Testicular Inhibition of Tyrosine Hydroxylase Expression in Tuberoinfundibular and Nigrostriatal Dopaminergic Neurons. Endocrinology. 1991;129(2):877-82. PubMed PMID: 1677330.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Testicular inhibition of tyrosine hydroxylase expression in tuberoinfundibular and nigrostriatal dopaminergic neurons. AU - Aguila-Mansilla,N, AU - Kedzierski,W, AU - Porter,J C, PY - 1991/8/1/pubmed PY - 1991/8/1/medline PY - 1991/8/1/entrez SP - 877 EP - 82 JF - Endocrinology JO - Endocrinology VL - 129 IS - 2 N2 - We have investigated, in young and aged rats, tuberoinfundibular dopaminergic (TIDA) and nigrostriatal neurons of the brain, and the role of the testes and anterior pituitary (AP) on 1) the in situ activity of tyrosine hydroxylase (TH), 2) translation of TH mRNA, as reflected by the mass of TH, and 3) transcription of the TH gene, as revealed by the mass of TH mRNA. The median eminence (ME) and corpus striatum (CS) were used as the sources of proximal neurites of the TIDA neurons and nigrostriatal neurons, respectively. The arcuate-periventricular nuclei of the ventral hypothalamus were used as the source of perikarya of the TIDA neurons, and substantia nigra (SN) nuclei of the midbrain were used as the source of perikarya of nigrostriatal neurons. The in situ activity of TH was calculated using the rate of accumulation of dihydroxyphenylalanine after pharmacological inhibition of dihydroxyphenylalanine decarboxylase activity. TH mass and TH mRNA were measured using an immunoblot assay and an S1 nuclease protection assay, respectively. Compared to intact animals, orchidectomized young and aged male rats had significantly (P less than 0.001) increased in situ activity of TH in the ME and CS, but not the SN. Orchidectomy also caused a significant (P less than 0.01) increase in the quantity of TH in the ME and a 2- to 3-fold increase in TH in the CS and SN. In contrast to castration, AP grafts, implanted in the lateral ventricles of the brain, caused a significant (P less than 0.001) increase only in TH activity in the ME. No effect of AP grafts was seen on TH activity of the CS or SN. AP grafts had no effect on the amount of TH in the ME, CS, or SN. In the TIDA neurons of young males and in the nigrostriatal neurons of young and aged castrates, the amount of TH mRNA was not different from that in intact males. AP grafts had no effect on TH mRNA in these dopaminergic neurons. These studies show that TIDA neurons and nigrostriatal neurons share some common aspects in their regulation and are dissimilar in others. AP grafts stimulate TH activity in young as well as aged TIDA neurons, but not in nigrostriatal neurons. Castration leads to an increase in TH mass in both TIDA and nigrostriatal neurons. The increase in TH mass is not associated with an increase in the amount of TH mRNA. It is suggested that castration results in increased translation of TH mRNA, but not transcription of the TH gene.(ABSTRACT TRUNCATED AT 400 WORDS) SN - 0013-7227 UR - https://www.unboundmedicine.com/medline/citation/1677330/Testicular_inhibition_of_tyrosine_hydroxylase_expression_in_tuberoinfundibular_and_nigrostriatal_dopaminergic_neurons_ L2 - https://academic.oup.com/endo/article-lookup/doi/10.1210/endo-129-2-877 DB - PRIME DP - Unbound Medicine ER -