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[Profile of toxigenicity of Clostridium difficile strains isolated from paediatric patients with clinical diagnosis of antibiotic associated diarrhea (AAD)].
Med Dosw Mikrobiol. 2005; 57(4):377-82.MD

Abstract

This study was performed to determine profile of toxigenicity of 18 Clostridium difficile strains isolated from paeditric patients suffering from antibiotic associated diarrhea (AAD). Toxigenicity of C. difficile strains was tested for detection toxin A and toxin B by phenotypic methods and for detection of the tcdA and tcdB genes using of PCR. Changes in the repeating regions of the tcdA genes were detected with the NK9/NKV011 primer pairs. For detection of binary toxin (CDT) cdtA and cdtB genes, cdtApos/cdtArev i cdtBpos/cdtBrev two pair primers in PCR was used. Among C. difficile strains was detected three profiles of toxigenicity: C. difficile strains possesing of tcdA and tcdB genes but not possesing cdtA and cdtB genes of binary toxin (A+B+CDT-), strains possesing tcdA and tcdB and cdtA and cdtB genes (A+B+CDT+), strains with deletion of toxin A gene (A-B+CDT-). This is the first report on the occurence of binary positive C. difficile strains isolated from paediatric patients.

Authors+Show Affiliations

Katedra i Zakład Mikrobiologii Lekarskiej AM w Warszawie.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

pol

PubMed ID

16773831

Citation

Wultańska, Dorota, et al. "[Profile of Toxigenicity of Clostridium Difficile Strains Isolated From Paediatric Patients With Clinical Diagnosis of Antibiotic Associated Diarrhea (AAD)]." Medycyna Doswiadczalna I Mikrobiologia, vol. 57, no. 4, 2005, pp. 377-82.
Wultańska D, Pituch H, Obuch-Woszczatyński P, et al. [Profile of toxigenicity of Clostridium difficile strains isolated from paediatric patients with clinical diagnosis of antibiotic associated diarrhea (AAD)]. Med Dosw Mikrobiol. 2005;57(4):377-82.
Wultańska, D., Pituch, H., Obuch-Woszczatyński, P., Meisel-Mikołajczyk, F., & Luczak, M. (2005). [Profile of toxigenicity of Clostridium difficile strains isolated from paediatric patients with clinical diagnosis of antibiotic associated diarrhea (AAD)]. Medycyna Doswiadczalna I Mikrobiologia, 57(4), 377-82.
Wultańska D, et al. [Profile of Toxigenicity of Clostridium Difficile Strains Isolated From Paediatric Patients With Clinical Diagnosis of Antibiotic Associated Diarrhea (AAD)]. Med Dosw Mikrobiol. 2005;57(4):377-82. PubMed PMID: 16773831.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Profile of toxigenicity of Clostridium difficile strains isolated from paediatric patients with clinical diagnosis of antibiotic associated diarrhea (AAD)]. AU - Wultańska,Dorota, AU - Pituch,Hanna, AU - Obuch-Woszczatyński,Piotr, AU - Meisel-Mikołajczyk,Felicja, AU - Luczak,Mirosław, PY - 2006/6/16/pubmed PY - 2006/8/4/medline PY - 2006/6/16/entrez SP - 377 EP - 82 JF - Medycyna doswiadczalna i mikrobiologia JO - Med Dosw Mikrobiol VL - 57 IS - 4 N2 - This study was performed to determine profile of toxigenicity of 18 Clostridium difficile strains isolated from paeditric patients suffering from antibiotic associated diarrhea (AAD). Toxigenicity of C. difficile strains was tested for detection toxin A and toxin B by phenotypic methods and for detection of the tcdA and tcdB genes using of PCR. Changes in the repeating regions of the tcdA genes were detected with the NK9/NKV011 primer pairs. For detection of binary toxin (CDT) cdtA and cdtB genes, cdtApos/cdtArev i cdtBpos/cdtBrev two pair primers in PCR was used. Among C. difficile strains was detected three profiles of toxigenicity: C. difficile strains possesing of tcdA and tcdB genes but not possesing cdtA and cdtB genes of binary toxin (A+B+CDT-), strains possesing tcdA and tcdB and cdtA and cdtB genes (A+B+CDT+), strains with deletion of toxin A gene (A-B+CDT-). This is the first report on the occurence of binary positive C. difficile strains isolated from paediatric patients. SN - 0025-8601 UR - https://www.unboundmedicine.com/medline/citation/16773831/[Profile_of_toxigenicity_of_Clostridium_difficile_strains_isolated_from_paediatric_patients_with_clinical_diagnosis_of_antibiotic_associated_diarrhea__AAD_]_ L2 - http://www.diseaseinfosearch.org/result/1688 DB - PRIME DP - Unbound Medicine ER -