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[The application of immunoperoxidase test for the detection of antibodies various classes (IgA, IgG and IgM) coating bacteria in urine].
Med Dosw Mikrobiol. 2005; 57(4):431-6.MD

Abstract

For the detection of bacteria coated with immunoglobulins in urine the monoclonal antibodies against human IgA, IgG and IgM conjugated with peroxidase were used. For comparison, the immunofluorescence technique was also employed. The results obtained by two methods revealed that immunofluorescence were less sensitive. It was found that bacteria were predominantly coated with IgA (41,9 +/- 22,4%) and IgG (34,1 +/- 15,3 %) immunoglobulins. The IgM antibodies were found rarely (12,8 +/- 8%).

Authors+Show Affiliations

Zakład Diagnostyki Mikrobiologicznej Akademii Medycznej w Białymstoku.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article

Language

pol

PubMed ID

16773837

Citation

Jakoniuk, Piotr, et al. "[The Application of Immunoperoxidase Test for the Detection of Antibodies Various Classes (IgA, IgG and IgM) Coating Bacteria in Urine]." Medycyna Doswiadczalna I Mikrobiologia, vol. 57, no. 4, 2005, pp. 431-6.
Jakoniuk P, Leszczyńska K, Zalewska M, et al. [The application of immunoperoxidase test for the detection of antibodies various classes (IgA, IgG and IgM) coating bacteria in urine]. Med Dosw Mikrobiol. 2005;57(4):431-6.
Jakoniuk, P., Leszczyńska, K., Zalewska, M., Sacha, P., & Wieczorek, P. (2005). [The application of immunoperoxidase test for the detection of antibodies various classes (IgA, IgG and IgM) coating bacteria in urine]. Medycyna Doswiadczalna I Mikrobiologia, 57(4), 431-6.
Jakoniuk P, et al. [The Application of Immunoperoxidase Test for the Detection of Antibodies Various Classes (IgA, IgG and IgM) Coating Bacteria in Urine]. Med Dosw Mikrobiol. 2005;57(4):431-6. PubMed PMID: 16773837.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [The application of immunoperoxidase test for the detection of antibodies various classes (IgA, IgG and IgM) coating bacteria in urine]. AU - Jakoniuk,Piotr, AU - Leszczyńska,Katarzyna, AU - Zalewska,Marta, AU - Sacha,Paweł, AU - Wieczorek,Piotr, PY - 2006/6/16/pubmed PY - 2006/8/4/medline PY - 2006/6/16/entrez SP - 431 EP - 6 JF - Medycyna doswiadczalna i mikrobiologia JO - Med Dosw Mikrobiol VL - 57 IS - 4 N2 - For the detection of bacteria coated with immunoglobulins in urine the monoclonal antibodies against human IgA, IgG and IgM conjugated with peroxidase were used. For comparison, the immunofluorescence technique was also employed. The results obtained by two methods revealed that immunofluorescence were less sensitive. It was found that bacteria were predominantly coated with IgA (41,9 +/- 22,4%) and IgG (34,1 +/- 15,3 %) immunoglobulins. The IgM antibodies were found rarely (12,8 +/- 8%). SN - 0025-8601 UR - https://www.unboundmedicine.com/medline/citation/16773837/[The_application_of_immunoperoxidase_test_for_the_detection_of_antibodies_various_classes__IgA_IgG_and_IgM__coating_bacteria_in_urine]_ L2 - https://medlineplus.gov/urineandurination.html DB - PRIME DP - Unbound Medicine ER -