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Time and sample site dependency of the optimized co-rebreathing method.
Med Sci Sports Exerc. 2006 Jun; 38(6):1187-93.MS

Abstract

PURPOSE

A new method to estimate hemoglobin mass (Hbmass) requires capillary blood and rebreathing a carbon-monoxide (CO) bolus for 2 min. We hypothesized that incomplete circulatory mixing of CO could confound this method, so we compared capillary with venous blood to determine whether sampling site altered the percentage of carboxyhemoglobin (%HbCO) and the reliability and accuracy of the "2-min Hbmass." The conventional 20-min CO-rebreathing procedure was used as the Hbmass criterion.

METHODS

In the first experiment (N=12), both fingertip capillary and antecubital venous blood were sampled 4 and 6 min after commencing 2 min of CO-rebreathing. Within 8 d, these subjects completed two 2-min and one 20-min CO-rebreathing periods. For the latter, capillary and venous blood were collected simultaneously after two 10-min periods of rebreathing. In a second experiment (N=6), both capillary and venous blood were sampled 4, 6, 8, 10, and 12 min after commencing 2 min of CO-rebreathing. A third experiment (N=6) evaluated the reliability of a modified 2-min CO-rebreathing test with capillary blood sampled at minutes 8 and 10.

RESULTS

Typical error (TE) for the first two 2-min tests was 1.1% (90% confidence limits 0.9-1.8%), but the average Hbmass from 2-min capillary blood was 4.8% lower than from venous blood for the 20-min procedure. In the second experiment, peak venous %HbCO occurred at minute 6, and the difference between capillary and venous values was minimal (mean+/-SD; 0.08+/-0.07, 0.01+/-0.09) at minutes 8 and 10. TE for the third experiment was 1.2% (0.8-2.5%).

CONCLUSION

A modified 2-min CO-rebreathing procedure using capillary or venous blood sampled 8 and 10 min after starting CO-rebreathing allows complete circulatory mixing and provides an accurate and reliable estimate of Hbmass.

Authors+Show Affiliations

Gore CJ
Department of Physiology, Australian Institute of Sport, Canberra, AUSTRALIA. chris.gore@ausort.gov.au
Bourdon PC
No affiliation info available
Woolford SM
No affiliation info available
Ostler LM
No affiliation info available
Eastwood A
No affiliation info available
Scroop GC
No affiliation info available

MeSH

AdultBlood CirculationBlood VolumeCapillariesCarbon MonoxideCarboxyhemoglobinConfounding Factors, EpidemiologicFemaleHemoglobinsHumansMalePhlebotomyReproducibility of ResultsTime FactorsVeins

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16775562

Citation

Gore, Christopher J., et al. "Time and Sample Site Dependency of the Optimized Co-rebreathing Method." Medicine and Science in Sports and Exercise, vol. 38, no. 6, 2006, pp. 1187-93.
Gore CJ, Bourdon PC, Woolford SM, et al. Time and sample site dependency of the optimized co-rebreathing method. Med Sci Sports Exerc. 2006;38(6):1187-93.
Gore, C. J., Bourdon, P. C., Woolford, S. M., Ostler, L. M., Eastwood, A., & Scroop, G. C. (2006). Time and sample site dependency of the optimized co-rebreathing method. Medicine and Science in Sports and Exercise, 38(6), 1187-93.
Gore CJ, et al. Time and Sample Site Dependency of the Optimized Co-rebreathing Method. Med Sci Sports Exerc. 2006;38(6):1187-93. PubMed PMID: 16775562.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Time and sample site dependency of the optimized co-rebreathing method. AU - Gore,Christopher J, AU - Bourdon,Pitre C, AU - Woolford,Sarah M, AU - Ostler,Laura M, AU - Eastwood,Annette, AU - Scroop,Garry C, PY - 2006/6/16/pubmed PY - 2006/10/28/medline PY - 2006/6/16/entrez SP - 1187 EP - 93 JF - Medicine and science in sports and exercise JO - Med Sci Sports Exerc VL - 38 IS - 6 N2 - PURPOSE: A new method to estimate hemoglobin mass (Hbmass) requires capillary blood and rebreathing a carbon-monoxide (CO) bolus for 2 min. We hypothesized that incomplete circulatory mixing of CO could confound this method, so we compared capillary with venous blood to determine whether sampling site altered the percentage of carboxyhemoglobin (%HbCO) and the reliability and accuracy of the "2-min Hbmass." The conventional 20-min CO-rebreathing procedure was used as the Hbmass criterion. METHODS: In the first experiment (N=12), both fingertip capillary and antecubital venous blood were sampled 4 and 6 min after commencing 2 min of CO-rebreathing. Within 8 d, these subjects completed two 2-min and one 20-min CO-rebreathing periods. For the latter, capillary and venous blood were collected simultaneously after two 10-min periods of rebreathing. In a second experiment (N=6), both capillary and venous blood were sampled 4, 6, 8, 10, and 12 min after commencing 2 min of CO-rebreathing. A third experiment (N=6) evaluated the reliability of a modified 2-min CO-rebreathing test with capillary blood sampled at minutes 8 and 10. RESULTS: Typical error (TE) for the first two 2-min tests was 1.1% (90% confidence limits 0.9-1.8%), but the average Hbmass from 2-min capillary blood was 4.8% lower than from venous blood for the 20-min procedure. In the second experiment, peak venous %HbCO occurred at minute 6, and the difference between capillary and venous values was minimal (mean+/-SD; 0.08+/-0.07, 0.01+/-0.09) at minutes 8 and 10. TE for the third experiment was 1.2% (0.8-2.5%). CONCLUSION: A modified 2-min CO-rebreathing procedure using capillary or venous blood sampled 8 and 10 min after starting CO-rebreathing allows complete circulatory mixing and provides an accurate and reliable estimate of Hbmass. SN - 0195-9131 UR - https://www.unboundmedicine.com/medline/citation/16775562/Time_and_sample_site_dependency_of_the_optimized_co_rebreathing_method_ DB - PRIME DP - Unbound Medicine ER -