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Cloning and molecular characterization of three novel LMW-i glutenin subunit genes from cultivated einkorn (Triticum monococcum L.).
Theor Appl Genet. 2006 Aug; 113(3):383-95.TA

Abstract

Three novel low molecular weight (LMW) glutenin subunits from cultivated einkorn (Triticum monococcum L., A(m)A(m), 2n = 2x = 14) were characterized by SDS-PAGE and molecular weights determined by MALDI-TOF-MS. Their coding genes were amplified and cloned with designed AS-PCR primers, revealing three complete gene sequences. All comprised upstream, open reading frame (ORF), downstream and no introns were present. The deduced amino acid sequences showed that all three genes, named as LMW-M1, LMW-M3 and LMW-M5, respectively, belonged to the LMW-i type subunits with the predicted molecular weight between 38.5206 and 38.7028 kDa. They showed high similarity with other LMW-i type genes from hexaploid bread wheats, but also displayed unique features. Particularly, LMW-M5 subunit contained an extra cysteine residue in the C-terminus except for eight conserved cysteines, which resulted from a single-nucleotide polymorphism (SNP) of the T-C transition, namely arginine --> cysteine substitution at position 242 from the N-terminal end. This is the first report that the LMW-i subunit contained nine cysteines residues that could result in a more highly cross-linked and more elastic glutenin suggesting that LMW-M5 gene may associates with good quality properties. In addition, a total of 25 SNPs and one insertions/deletions (InDels) were detected among three LMW-i genes, which could result in significant functional changes in polymer formation of gluten. It is anticipated that these SNPs could be used as reliable genetic markers during wheat quality improvement. The phylogenetic analysis indicated that LMW-i type genes apparently differed from LMW-m and LMW-s type genes and diverged early from the primitive LMW-GS gene family, at about 12.92 million years ago (MYA) while the differentiation of A(m) and A genomes was estimated at 3.98 MYA.

Authors+Show Affiliations

An X
Key Laboratory of Genetics and Biotechnology, College of Life Science, Capital Normal University, 100037, Beijing, China.
Zhang Q
No affiliation info available
Yan Y
No affiliation info available
Li Q
No affiliation info available
Zhang Y
No affiliation info available
Wang A
No affiliation info available
Pei Y
No affiliation info available
Tian J
No affiliation info available
Wang H
No affiliation info available
Hsam SL
No affiliation info available
Zeller FJ
No affiliation info available

MeSH

Amino Acid SequenceBase CompositionCloning, MolecularGene DeletionGenetic MarkersGlutensMolecular Sequence DataMutagenesis, InsertionalPhylogenyPlant ProteinsPolymerase Chain ReactionPolymorphism, Single NucleotideProlaminsProtein SubunitsSequence AlignmentTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16775696

Citation

An, X, et al. "Cloning and Molecular Characterization of Three Novel LMW-i Glutenin Subunit Genes From Cultivated Einkorn (Triticum Monococcum L.)." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 113, no. 3, 2006, pp. 383-95.
An X, Zhang Q, Yan Y, et al. Cloning and molecular characterization of three novel LMW-i glutenin subunit genes from cultivated einkorn (Triticum monococcum L.). Theor Appl Genet. 2006;113(3):383-95.
An, X., Zhang, Q., Yan, Y., Li, Q., Zhang, Y., Wang, A., Pei, Y., Tian, J., Wang, H., Hsam, S. L., & Zeller, F. J. (2006). Cloning and molecular characterization of three novel LMW-i glutenin subunit genes from cultivated einkorn (Triticum monococcum L.). TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 113(3), 383-95.
An X, et al. Cloning and Molecular Characterization of Three Novel LMW-i Glutenin Subunit Genes From Cultivated Einkorn (Triticum Monococcum L.). Theor Appl Genet. 2006;113(3):383-95. PubMed PMID: 16775696.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cloning and molecular characterization of three novel LMW-i glutenin subunit genes from cultivated einkorn (Triticum monococcum L.). AU - An,X, AU - Zhang,Q, AU - Yan,Y, AU - Li,Q, AU - Zhang,Y, AU - Wang,A, AU - Pei,Y, AU - Tian,J, AU - Wang,H, AU - Hsam,S L K, AU - Zeller,F J, Y1 - 2006/06/15/ PY - 2005/10/21/received PY - 2006/04/19/accepted PY - 2006/6/16/pubmed PY - 2006/10/20/medline PY - 2006/6/16/entrez SP - 383 EP - 95 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 113 IS - 3 N2 - Three novel low molecular weight (LMW) glutenin subunits from cultivated einkorn (Triticum monococcum L., A(m)A(m), 2n = 2x = 14) were characterized by SDS-PAGE and molecular weights determined by MALDI-TOF-MS. Their coding genes were amplified and cloned with designed AS-PCR primers, revealing three complete gene sequences. All comprised upstream, open reading frame (ORF), downstream and no introns were present. The deduced amino acid sequences showed that all three genes, named as LMW-M1, LMW-M3 and LMW-M5, respectively, belonged to the LMW-i type subunits with the predicted molecular weight between 38.5206 and 38.7028 kDa. They showed high similarity with other LMW-i type genes from hexaploid bread wheats, but also displayed unique features. Particularly, LMW-M5 subunit contained an extra cysteine residue in the C-terminus except for eight conserved cysteines, which resulted from a single-nucleotide polymorphism (SNP) of the T-C transition, namely arginine --> cysteine substitution at position 242 from the N-terminal end. This is the first report that the LMW-i subunit contained nine cysteines residues that could result in a more highly cross-linked and more elastic glutenin suggesting that LMW-M5 gene may associates with good quality properties. In addition, a total of 25 SNPs and one insertions/deletions (InDels) were detected among three LMW-i genes, which could result in significant functional changes in polymer formation of gluten. It is anticipated that these SNPs could be used as reliable genetic markers during wheat quality improvement. The phylogenetic analysis indicated that LMW-i type genes apparently differed from LMW-m and LMW-s type genes and diverged early from the primitive LMW-GS gene family, at about 12.92 million years ago (MYA) while the differentiation of A(m) and A genomes was estimated at 3.98 MYA. SN - 0040-5752 UR - https://www.unboundmedicine.com/medline/citation/16775696/Cloning_and_molecular_characterization_of_three_novel_LMW_i_glutenin_subunit_genes_from_cultivated_einkorn__Triticum_monococcum_L___ DB - PRIME DP - Unbound Medicine ER -