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Comparative sequence analysis of major envelope protein gene (B2L) of Indian orf viruses isolated from sheep and goats.
Vet Microbiol 2006; 116(4):317-24VM

Abstract

Orf virus (ORFV), the type species of Parapoxvirus, is responsible for contagious ecthyma in sheep and goats. In the present report, sequence analysis of major envelope gene (B2L) of four Indian orf virus isolates originating two each from sheep and goats was carried out. These recent isolates belonged to different outbreaks that occurred in Kumaon hills and adjoining plains during 2004-2005. Preliminary screening of the scab samples was carried out by diagnostic PCR. Full-length B2L gene encoding for immunogenic major envelope protein from all the four ORFV isolates was amplified by PCR and the amplicons (1206 bp) were cloned and sequenced. Comparative sequence analysis revealed an open reading frame of 1137 nucleotides (nt) encoding a polypeptide of 378 amino acids (aa). Indian isolates were highly related amongst themselves with sequence identity of over 97% at the nt and aa level. Further, they showed 97-98% sequence identity with sequences of other ORFV isolates from around the world; while 94-95 and 82.7-83.8% sequence identity was observed, respectively, with pseudocowpox and bovine papular stomatitis viruses--the other members of the genus. Phylogenetic analysis also showed that these Parapoxviruses from sheep and goats are closely related to other orf viruses reported worldwide.

Authors+Show Affiliations

Division of Virology, Indian Veterinary Research Institute, Mukteswar Campus, Mukteswar, Nainital 263138, Uttaranchal, India. m_hosa@email.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

16777357

Citation

Hosamani, Madhusudan, et al. "Comparative Sequence Analysis of Major Envelope Protein Gene (B2L) of Indian Orf Viruses Isolated From Sheep and Goats." Veterinary Microbiology, vol. 116, no. 4, 2006, pp. 317-24.
Hosamani M, Bhanuprakash V, Scagliarini A, et al. Comparative sequence analysis of major envelope protein gene (B2L) of Indian orf viruses isolated from sheep and goats. Vet Microbiol. 2006;116(4):317-24.
Hosamani, M., Bhanuprakash, V., Scagliarini, A., & Singh, R. K. (2006). Comparative sequence analysis of major envelope protein gene (B2L) of Indian orf viruses isolated from sheep and goats. Veterinary Microbiology, 116(4), pp. 317-24.
Hosamani M, et al. Comparative Sequence Analysis of Major Envelope Protein Gene (B2L) of Indian Orf Viruses Isolated From Sheep and Goats. Vet Microbiol. 2006 Sep 10;116(4):317-24. PubMed PMID: 16777357.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative sequence analysis of major envelope protein gene (B2L) of Indian orf viruses isolated from sheep and goats. AU - Hosamani,Madhusudan, AU - Bhanuprakash,V, AU - Scagliarini,A, AU - Singh,R K, Y1 - 2006/05/07/ PY - 2006/02/16/received PY - 2006/04/20/revised PY - 2006/04/28/accepted PY - 2006/6/17/pubmed PY - 2006/10/27/medline PY - 2006/6/17/entrez SP - 317 EP - 24 JF - Veterinary microbiology JO - Vet. Microbiol. VL - 116 IS - 4 N2 - Orf virus (ORFV), the type species of Parapoxvirus, is responsible for contagious ecthyma in sheep and goats. In the present report, sequence analysis of major envelope gene (B2L) of four Indian orf virus isolates originating two each from sheep and goats was carried out. These recent isolates belonged to different outbreaks that occurred in Kumaon hills and adjoining plains during 2004-2005. Preliminary screening of the scab samples was carried out by diagnostic PCR. Full-length B2L gene encoding for immunogenic major envelope protein from all the four ORFV isolates was amplified by PCR and the amplicons (1206 bp) were cloned and sequenced. Comparative sequence analysis revealed an open reading frame of 1137 nucleotides (nt) encoding a polypeptide of 378 amino acids (aa). Indian isolates were highly related amongst themselves with sequence identity of over 97% at the nt and aa level. Further, they showed 97-98% sequence identity with sequences of other ORFV isolates from around the world; while 94-95 and 82.7-83.8% sequence identity was observed, respectively, with pseudocowpox and bovine papular stomatitis viruses--the other members of the genus. Phylogenetic analysis also showed that these Parapoxviruses from sheep and goats are closely related to other orf viruses reported worldwide. SN - 0378-1135 UR - https://www.unboundmedicine.com/medline/citation/16777357/Comparative_sequence_analysis_of_major_envelope_protein_gene__B2L__of_Indian_orf_viruses_isolated_from_sheep_and_goats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1135(06)00182-9 DB - PRIME DP - Unbound Medicine ER -