Tags

Type your tag names separated by a space and hit enter

Crataegus special extract WS 1442 induces an endothelium-dependent, NO-mediated vasorelaxation via eNOS-phosphorylation at serine 1177.
Cardiovasc Drugs Ther. 2006 Jun; 20(3):177-84.CD

Abstract

PURPOSE

This study investigates the influence of WS(R) 1442, a special extract of Crataegus leaves with flowers, on the relaxation of rat aorta and human mammarian artery (coronary bypass patients).

METHODS

Experiments were performed in the presence and absence (mechanical disruption) of endothelium. In addition, we investigated three fractions of WS(R) 1442 (fraction A: lipophilic, containing flavonoids and oligomeric procyanidins (OPC), fraction B: hydrophilic, containing flavonoids and low molecular weight OPC, fraction C: hydrophilic, essentially flavonoid-free and rich in high molecular weight OPC).

RESULTS

WS 1442 induced a concentration-dependent vasodilation in isolated vessel rings that had been precontracted by 10 microM phenylephrine (concentration for halfmaximal relaxation (IC(50)): rat: 15.1 +/- 0.6 microg/ml (n = 7), human: 19.3 +/- 3.4 microg/ml (n = 6)). The maximal vasorelaxation induced after application of 100 microg of WS 1442 was 75.0 +/- 5.7% (rat) and 79.2 +/- 5.8% (human) of the papaverine (0.1 mM)-induced vasodilation. If the experiments were performed in the presence of L-nitroarginine methylester (10 microM, eNOS-inhibition) or after mechanical disruption of the endothelium, no vasorelaxation was observed in the presence of WS 1442. The vasorelaxant properties of WS 1442 were mediated by fraction C. WS 1442 induced an NO-liberation from human coronary artery endothelial cells as measured by diaminofluorescein. WS 1442 induced eNOS-activation was due to a phosphorylation at serine 1177. No eNOS-translocation or phosphorylation at serine 114 or threonine 495 was observed after application of WS 1442.

CONCLUSIONS

It is concluded that WS 1442, induces an endothelium-dependent, NO-mediated vasorelaxation via eNOS phosphorylation at serine 1177.

Authors+Show Affiliations

Brixius K
Laboratory of Muscle Research and Molecular Cardiology, Clinic III for Internal Medicine, University of Cologne, Joseph-Stelzmann-Str. 9, D-50924, Cologne, Germany, Robert.
Willms S
No affiliation info available
Napp A
No affiliation info available
Tossios P
No affiliation info available
Ladage D
No affiliation info available
Bloch W
No affiliation info available
Mehlhorn U
No affiliation info available
Schwinger RH
No affiliation info available

MeSH

AdultAnimalsAortaDose-Response Relationship, DrugEndothelium, VascularEnzyme ActivationFemaleFlavonoidsHumansMaleMiddle AgedNitric OxideNitric Oxide Synthase Type IIIPhosphorylationPlant ExtractsRatsRats, WistarSerineVasodilation

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16779533

Citation

Brixius, Klara, et al. "Crataegus Special Extract WS 1442 Induces an Endothelium-dependent, NO-mediated Vasorelaxation Via eNOS-phosphorylation at Serine 1177." Cardiovascular Drugs and Therapy, vol. 20, no. 3, 2006, pp. 177-84.
Brixius K, Willms S, Napp A, et al. Crataegus special extract WS 1442 induces an endothelium-dependent, NO-mediated vasorelaxation via eNOS-phosphorylation at serine 1177. Cardiovasc Drugs Ther. 2006;20(3):177-84.
Brixius, K., Willms, S., Napp, A., Tossios, P., Ladage, D., Bloch, W., Mehlhorn, U., & Schwinger, R. H. (2006). Crataegus special extract WS 1442 induces an endothelium-dependent, NO-mediated vasorelaxation via eNOS-phosphorylation at serine 1177. Cardiovascular Drugs and Therapy, 20(3), 177-84.
Brixius K, et al. Crataegus Special Extract WS 1442 Induces an Endothelium-dependent, NO-mediated Vasorelaxation Via eNOS-phosphorylation at Serine 1177. Cardiovasc Drugs Ther. 2006;20(3):177-84. PubMed PMID: 16779533.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Crataegus special extract WS 1442 induces an endothelium-dependent, NO-mediated vasorelaxation via eNOS-phosphorylation at serine 1177. AU - Brixius,Klara, AU - Willms,Sonja, AU - Napp,Andreas, AU - Tossios,Paschalios, AU - Ladage,Dennis, AU - Bloch,Wilhelm, AU - Mehlhorn,Uwe, AU - Schwinger,Robert H G, PY - 2006/6/17/pubmed PY - 2006/12/9/medline PY - 2006/6/17/entrez SP - 177 EP - 84 JF - Cardiovascular drugs and therapy JO - Cardiovasc Drugs Ther VL - 20 IS - 3 N2 - PURPOSE: This study investigates the influence of WS(R) 1442, a special extract of Crataegus leaves with flowers, on the relaxation of rat aorta and human mammarian artery (coronary bypass patients). METHODS: Experiments were performed in the presence and absence (mechanical disruption) of endothelium. In addition, we investigated three fractions of WS(R) 1442 (fraction A: lipophilic, containing flavonoids and oligomeric procyanidins (OPC), fraction B: hydrophilic, containing flavonoids and low molecular weight OPC, fraction C: hydrophilic, essentially flavonoid-free and rich in high molecular weight OPC). RESULTS: WS 1442 induced a concentration-dependent vasodilation in isolated vessel rings that had been precontracted by 10 microM phenylephrine (concentration for halfmaximal relaxation (IC(50)): rat: 15.1 +/- 0.6 microg/ml (n = 7), human: 19.3 +/- 3.4 microg/ml (n = 6)). The maximal vasorelaxation induced after application of 100 microg of WS 1442 was 75.0 +/- 5.7% (rat) and 79.2 +/- 5.8% (human) of the papaverine (0.1 mM)-induced vasodilation. If the experiments were performed in the presence of L-nitroarginine methylester (10 microM, eNOS-inhibition) or after mechanical disruption of the endothelium, no vasorelaxation was observed in the presence of WS 1442. The vasorelaxant properties of WS 1442 were mediated by fraction C. WS 1442 induced an NO-liberation from human coronary artery endothelial cells as measured by diaminofluorescein. WS 1442 induced eNOS-activation was due to a phosphorylation at serine 1177. No eNOS-translocation or phosphorylation at serine 114 or threonine 495 was observed after application of WS 1442. CONCLUSIONS: It is concluded that WS 1442, induces an endothelium-dependent, NO-mediated vasorelaxation via eNOS phosphorylation at serine 1177. SN - 0920-3206 UR - https://www.unboundmedicine.com/medline/citation/16779533/Crataegus_special_extract_WS_1442_induces_an_endothelium_dependent_NO_mediated_vasorelaxation_via_eNOS_phosphorylation_at_serine_1177_ DB - PRIME DP - Unbound Medicine ER -