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Quantification of MDMA and MDA in abusers' hair samples by semi-micro column HPLC with fluorescence detection.
Biomed Chromatogr. 2006 Jun-Jul; 20(6-7):622-7.BC

Abstract

A sensitive semi-micro column high-performance liquid chromatography with fluorescence detection method was developed for the determination of 3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxyamphetamine (MDA), methamphetamine (MP) and amphetamine (AP) in human hair. 4-(4,5-Diphenyl-1H-imidazol-2-yl)benzoyl chloride (DIB-Cl) and 1-methyl-3-phenylpropylamine were used as labeling reagent and internal standard, respectively. These drugs were extracted from hair into 5% trifluoroacetic acid in methanol, and fluorescent labeled with DIB-Cl. The separation of DIB-derivatives was achieved on a reversed-phase semi-micro ODS column with an acetonitrile-methanol-water (30:40:30, v/v/v%) mixture as a mobile phase. The limits of detection at a signal-to-noise ratio of 3 for MDMA, MDA, MP and AP were 0.25, 0.15, 0.25 and 0.19 ng/mg, respectively. Precision of intra- and inter-day assay as the relative standard deviation were in the range 1.5-6.8% (n = 5) and 2.7-4.7% (n = 5), respectively. The proposed method was highly sensitive and able to detect MDMA and its related compounds in small amounts of hair sample, and could be applied to quantification of six abusers' hair samples.

Authors+Show Affiliations

Department of Clinical Pharmacy, Course of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

16779783

Citation

Nakamura, Shinichi, et al. "Quantification of MDMA and MDA in Abusers' Hair Samples By Semi-micro Column HPLC With Fluorescence Detection." Biomedical Chromatography : BMC, vol. 20, no. 6-7, 2006, pp. 622-7.
Nakamura S, Tomita M, Wada M, et al. Quantification of MDMA and MDA in abusers' hair samples by semi-micro column HPLC with fluorescence detection. Biomed Chromatogr. 2006;20(6-7):622-7.
Nakamura, S., Tomita, M., Wada, M., Chung, H., Kuroda, N., & Nakashima, K. (2006). Quantification of MDMA and MDA in abusers' hair samples by semi-micro column HPLC with fluorescence detection. Biomedical Chromatography : BMC, 20(6-7), 622-7.
Nakamura S, et al. Quantification of MDMA and MDA in Abusers' Hair Samples By Semi-micro Column HPLC With Fluorescence Detection. Biomed Chromatogr. 2006 Jun-Jul;20(6-7):622-7. PubMed PMID: 16779783.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantification of MDMA and MDA in abusers' hair samples by semi-micro column HPLC with fluorescence detection. AU - Nakamura,Shinichi, AU - Tomita,Mamoru, AU - Wada,Mitsuhiro, AU - Chung,Heesun, AU - Kuroda,Naotaka, AU - Nakashima,Kenichiro, PY - 2006/6/17/pubmed PY - 2006/8/29/medline PY - 2006/6/17/entrez SP - 622 EP - 7 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 20 IS - 6-7 N2 - A sensitive semi-micro column high-performance liquid chromatography with fluorescence detection method was developed for the determination of 3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxyamphetamine (MDA), methamphetamine (MP) and amphetamine (AP) in human hair. 4-(4,5-Diphenyl-1H-imidazol-2-yl)benzoyl chloride (DIB-Cl) and 1-methyl-3-phenylpropylamine were used as labeling reagent and internal standard, respectively. These drugs were extracted from hair into 5% trifluoroacetic acid in methanol, and fluorescent labeled with DIB-Cl. The separation of DIB-derivatives was achieved on a reversed-phase semi-micro ODS column with an acetonitrile-methanol-water (30:40:30, v/v/v%) mixture as a mobile phase. The limits of detection at a signal-to-noise ratio of 3 for MDMA, MDA, MP and AP were 0.25, 0.15, 0.25 and 0.19 ng/mg, respectively. Precision of intra- and inter-day assay as the relative standard deviation were in the range 1.5-6.8% (n = 5) and 2.7-4.7% (n = 5), respectively. The proposed method was highly sensitive and able to detect MDMA and its related compounds in small amounts of hair sample, and could be applied to quantification of six abusers' hair samples. SN - 0269-3879 UR - https://www.unboundmedicine.com/medline/citation/16779783/Quantification_of_MDMA_and_MDA_in_abusers'_hair_samples_by_semi_micro_column_HPLC_with_fluorescence_detection_ L2 - https://doi.org/10.1002/bmc.670 DB - PRIME DP - Unbound Medicine ER -