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Induction and activity of oxidative stress-related proteins during waterborne Cu-exposure in brown trout (Salmo trutta).
Chemosphere. 2006 Dec; 65(10):1707-14.C

Abstract

Induction of gene transcription for proteins and enzymes involved in metal-mediated oxidative stress were studied in brown trout transferred to a Cu-contaminated river in the Røros region in Central Norway. In addition to metallothionein (MT-A), Cu/Zn-superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR) gene transcription, protein levels of MT and enzyme activities of SOD and CAT were analyzed in gill, liver and kidney. MT-A, SOD and GR transcription increased significantly along with uptake of Cu in gills, while only transcription of MT-A was found to respond in liver and kidney during the exposure. Already present MT proteins in gills seemed to be oxidized during the exposure, probably caused by Cu-mediated oxidative stress, and no increase in MT protein levels were observed in gills. SOD and CAT enzyme levels were affected in all tissues during the exposure. A negative correlation between SOD and CAT activities was observed in gills, and we suggest that the activities of these enzymes were influenced not only through transcription. GPx and GR transcription levels correlated positively with each other in gills and liver, indicating their shared function in GSH-turnover. Levels of MT and activity of SOD and CAT dealing with metal-induced oxidative stress appear to be regulated not only through gene transcription, but also through post-translational mechanisms.

Authors+Show Affiliations

Norwegian University of Science and Technology (NTNU), Department of Biology, Høgskoleringen 5, N-7491 Trondheim, Norway. bjorn.henrik.hansen@bio.ntnu.noNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16780922

Citation

Hansen, Bjørn Henrik, et al. "Induction and Activity of Oxidative Stress-related Proteins During Waterborne Cu-exposure in Brown Trout (Salmo Trutta)." Chemosphere, vol. 65, no. 10, 2006, pp. 1707-14.
Hansen BH, Rømma S, Søfteland LI, et al. Induction and activity of oxidative stress-related proteins during waterborne Cu-exposure in brown trout (Salmo trutta). Chemosphere. 2006;65(10):1707-14.
Hansen, B. H., Rømma, S., Søfteland, L. I., Olsvik, P. A., & Andersen, R. A. (2006). Induction and activity of oxidative stress-related proteins during waterborne Cu-exposure in brown trout (Salmo trutta). Chemosphere, 65(10), 1707-14.
Hansen BH, et al. Induction and Activity of Oxidative Stress-related Proteins During Waterborne Cu-exposure in Brown Trout (Salmo Trutta). Chemosphere. 2006;65(10):1707-14. PubMed PMID: 16780922.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Induction and activity of oxidative stress-related proteins during waterborne Cu-exposure in brown trout (Salmo trutta). AU - Hansen,Bjørn Henrik, AU - Rømma,Svein, AU - Søfteland,Liv I R, AU - Olsvik,Pål A, AU - Andersen,Rolf A, Y1 - 2006/06/14/ PY - 2006/01/27/received PY - 2006/04/26/revised PY - 2006/04/28/accepted PY - 2006/6/20/pubmed PY - 2007/4/11/medline PY - 2006/6/20/entrez SP - 1707 EP - 14 JF - Chemosphere JO - Chemosphere VL - 65 IS - 10 N2 - Induction of gene transcription for proteins and enzymes involved in metal-mediated oxidative stress were studied in brown trout transferred to a Cu-contaminated river in the Røros region in Central Norway. In addition to metallothionein (MT-A), Cu/Zn-superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR) gene transcription, protein levels of MT and enzyme activities of SOD and CAT were analyzed in gill, liver and kidney. MT-A, SOD and GR transcription increased significantly along with uptake of Cu in gills, while only transcription of MT-A was found to respond in liver and kidney during the exposure. Already present MT proteins in gills seemed to be oxidized during the exposure, probably caused by Cu-mediated oxidative stress, and no increase in MT protein levels were observed in gills. SOD and CAT enzyme levels were affected in all tissues during the exposure. A negative correlation between SOD and CAT activities was observed in gills, and we suggest that the activities of these enzymes were influenced not only through transcription. GPx and GR transcription levels correlated positively with each other in gills and liver, indicating their shared function in GSH-turnover. Levels of MT and activity of SOD and CAT dealing with metal-induced oxidative stress appear to be regulated not only through gene transcription, but also through post-translational mechanisms. SN - 0045-6535 UR - https://www.unboundmedicine.com/medline/citation/16780922/Induction_and_activity_of_oxidative_stress_related_proteins_during_waterborne_Cu_exposure_in_brown_trout__Salmo_trutta__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0045-6535(06)00579-0 DB - PRIME DP - Unbound Medicine ER -