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Detection of Escherichia coli O157:H7 using chicken immunoglobulin Y.
Immunol Lett. 2006 Aug 15; 106(2):191-3.IL

Abstract

A sandwich ELISA technique was examined to detect Escherichia coli O157:H7 using chicken anti-E. coli O157:H7 IgY as the capture-antibody and an anti-E. coli O157 mouse mAb conjugated with biotin as the detection antibody. The anti-E. coli O157:H7 IgY was harvested from eggs laid by hens (23 weeks of age, Single Comb White Leghorn) immunized with formalin-killed E. coli O157:H7. The IgY was purified by water dilution methods and gel chromatography on Sephacryl S-300 followed by ammonium sulfate precipitation. The sensitivity (CFU/ml) of sandwich ELISA for the E. coli O157:H7 was repeatedly examined with 10 replicates of each sample and a standard curve was plotted. The sandwich ELISA can detect as low as 40CFU/ml of E. coli O157:H7. The data suggest that chicken IgY-based sandwich ELISA provides a reliable, inexpensive and sensitive assay for the detection of the food-borne pathogen E. coli O157:H7.

Authors+Show Affiliations

Sunwoo HH
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Canada AB T6G 2P5. hsunwoo@ualberta.ca <hsunwoo@ualberta.ca>
Wang WW
No affiliation info available
Sim JS
No affiliation info available

MeSH

AnimalsAntibodies, BacterialBacterial Typing TechniquesChickensEnzyme-Linked Immunosorbent AssayEscherichia coli O157Food MicrobiologyImmunoglobulinsSensitivity and Specificity

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16781781

Citation

Sunwoo, Hoon H., et al. "Detection of Escherichia Coli O157:H7 Using Chicken Immunoglobulin Y." Immunology Letters, vol. 106, no. 2, 2006, pp. 191-3.
Sunwoo HH, Wang WW, Sim JS. Detection of Escherichia coli O157:H7 using chicken immunoglobulin Y. Immunol Lett. 2006;106(2):191-3.
Sunwoo, H. H., Wang, W. W., & Sim, J. S. (2006). Detection of Escherichia coli O157:H7 using chicken immunoglobulin Y. Immunology Letters, 106(2), 191-3.
Sunwoo HH, Wang WW, Sim JS. Detection of Escherichia Coli O157:H7 Using Chicken Immunoglobulin Y. Immunol Lett. 2006 Aug 15;106(2):191-3. PubMed PMID: 16781781.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of Escherichia coli O157:H7 using chicken immunoglobulin Y. AU - Sunwoo,Hoon H, AU - Wang,Welson W, AU - Sim,Jeong S, Y1 - 2006/06/02/ PY - 2005/12/13/received PY - 2006/04/11/revised PY - 2006/05/10/accepted PY - 2006/6/20/pubmed PY - 2006/11/1/medline PY - 2006/6/20/entrez SP - 191 EP - 3 JF - Immunology letters JO - Immunol Lett VL - 106 IS - 2 N2 - A sandwich ELISA technique was examined to detect Escherichia coli O157:H7 using chicken anti-E. coli O157:H7 IgY as the capture-antibody and an anti-E. coli O157 mouse mAb conjugated with biotin as the detection antibody. The anti-E. coli O157:H7 IgY was harvested from eggs laid by hens (23 weeks of age, Single Comb White Leghorn) immunized with formalin-killed E. coli O157:H7. The IgY was purified by water dilution methods and gel chromatography on Sephacryl S-300 followed by ammonium sulfate precipitation. The sensitivity (CFU/ml) of sandwich ELISA for the E. coli O157:H7 was repeatedly examined with 10 replicates of each sample and a standard curve was plotted. The sandwich ELISA can detect as low as 40CFU/ml of E. coli O157:H7. The data suggest that chicken IgY-based sandwich ELISA provides a reliable, inexpensive and sensitive assay for the detection of the food-borne pathogen E. coli O157:H7. SN - 0165-2478 UR - https://www.unboundmedicine.com/medline/citation/16781781/Detection_of_Escherichia_coli_O157:H7_using_chicken_immunoglobulin_Y_ DB - PRIME DP - Unbound Medicine ER -