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Why not using capillary electrophoresis in drug analysis?
Electrophoresis. 2006 Jun; 27(12):2283-92.E

Abstract

CE and related methods are well-established techniques in the analysis of biomolecules, such as DNA and proteins. Even though CE is a rather good alternative to HPLC for the evaluation of the impurity profile and the enantiomeric purity of a drug, it is rarely applied. This might be due to the reservation of national licensing authorities and the pharmacopoeia commissions for several reasons. In this review containing some experimental data we report on several drug examples which demonstrate the superiority of CE over HPLC in special cases, i.e., in the analysis of antibiotics, amino acids and peptides, and the determination of enantiomeric purity. However, in order to make the CE techniques more suitable for pharmacopoeial purposes the general methods describing separation methods have to be complemented with the adjustment of the electrophoretic conditions being necessary to satisfy the system suitability criteria without fundamentally modifying the methods. Taken together CE should be more often applied in drug quality control.

Authors+Show Affiliations

Holzgrabe U
Institute of Pharmacy and Food Chemistry, University of Würzburg, Würzburg, Germany. u.holzgrabe@pharmazie.uni-wuerzburg.de
Brinz D
No affiliation info available
Kopec S
No affiliation info available
Weber C
No affiliation info available
Bitar Y
No affiliation info available

MeSH

Chromatography, High Pressure LiquidDrug ContaminationElectrophoresis, CapillaryPharmaceutical PreparationsStereoisomerism

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

16786478

Citation

Holzgrabe, Ulrike, et al. "Why Not Using Capillary Electrophoresis in Drug Analysis?" Electrophoresis, vol. 27, no. 12, 2006, pp. 2283-92.
Holzgrabe U, Brinz D, Kopec S, et al. Why not using capillary electrophoresis in drug analysis? Electrophoresis. 2006;27(12):2283-92.
Holzgrabe, U., Brinz, D., Kopec, S., Weber, C., & Bitar, Y. (2006). Why not using capillary electrophoresis in drug analysis? Electrophoresis, 27(12), 2283-92.
Holzgrabe U, et al. Why Not Using Capillary Electrophoresis in Drug Analysis. Electrophoresis. 2006;27(12):2283-92. PubMed PMID: 16786478.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Why not using capillary electrophoresis in drug analysis? AU - Holzgrabe,Ulrike, AU - Brinz,Daniela, AU - Kopec,Susanne, AU - Weber,Christine, AU - Bitar,Yaser, PY - 2006/6/21/pubmed PY - 2006/9/14/medline PY - 2006/6/21/entrez SP - 2283 EP - 92 JF - Electrophoresis JO - Electrophoresis VL - 27 IS - 12 N2 - CE and related methods are well-established techniques in the analysis of biomolecules, such as DNA and proteins. Even though CE is a rather good alternative to HPLC for the evaluation of the impurity profile and the enantiomeric purity of a drug, it is rarely applied. This might be due to the reservation of national licensing authorities and the pharmacopoeia commissions for several reasons. In this review containing some experimental data we report on several drug examples which demonstrate the superiority of CE over HPLC in special cases, i.e., in the analysis of antibiotics, amino acids and peptides, and the determination of enantiomeric purity. However, in order to make the CE techniques more suitable for pharmacopoeial purposes the general methods describing separation methods have to be complemented with the adjustment of the electrophoretic conditions being necessary to satisfy the system suitability criteria without fundamentally modifying the methods. Taken together CE should be more often applied in drug quality control. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/16786478/Why_not_using_capillary_electrophoresis_in_drug_analysis L2 - https://doi.org/10.1002/elps.200600016 DB - PRIME DP - Unbound Medicine ER -