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Characterization of flavonol conjugates in immature leaves of pak choi [Brassica rapa L. Ssp. chinensis L. (Hanelt.)] by HPLC-DAD and LC-MS/MS.
J Agric Food Chem. 2006 Jun 28; 54(13):4855-60.JA

Abstract

The flavonoid composition of immature leaves of pak choi [Brassica rapa L. ssp. chinensis L. (Hanelt.)] was investigated. Flavonol aglycone content was measured in 11 pak choi varieties, indicating significant differences (P < 0.05) in content between varieties and relatively high contents of kaempferol and isorhamnetin. Levels of quercetin ranged from 3.2 to 6.1 mg/100 g of dry weight (DW), whereas levels of isorhamnetin and kaempferol were significantly higher (8.1-35.1 and 36.0-102.6 mg/100 g of DW, respectively). A large number of glycoside and hydroxycinnamic acid derivatives of quercetin, kaempferol, and isorhamnetin were identified in cv. 'Shanghai' by LC/UV-DAD/ESI-MS/MS. The UV-DAD data allowed identification of hydroxycinnamic acid derivatives, but detailed MS/MS fragmentations were required for the structure elucidation. Pak choi could be a potentially important source of dietary flavonols, in particular, kaempferol and isorhamnetin.

Authors+Show Affiliations

Department of Primary Industries, Primary Industries Research Victoria, Werribee Centre, 621 Sneydes Road, Werribee 3030, Victoria, Australia. simone.rochfort@dpi.vic.gov.auNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16787039

Citation

Rochfort, Simone J., et al. "Characterization of Flavonol Conjugates in Immature Leaves of Pak Choi [Brassica Rapa L. Ssp. Chinensis L. (Hanelt.)] By HPLC-DAD and LC-MS/MS." Journal of Agricultural and Food Chemistry, vol. 54, no. 13, 2006, pp. 4855-60.
Rochfort SJ, Imsic M, Jones R, et al. Characterization of flavonol conjugates in immature leaves of pak choi [Brassica rapa L. Ssp. chinensis L. (Hanelt.)] by HPLC-DAD and LC-MS/MS. J Agric Food Chem. 2006;54(13):4855-60.
Rochfort, S. J., Imsic, M., Jones, R., Trenerry, V. C., & Tomkins, B. (2006). Characterization of flavonol conjugates in immature leaves of pak choi [Brassica rapa L. Ssp. chinensis L. (Hanelt.)] by HPLC-DAD and LC-MS/MS. Journal of Agricultural and Food Chemistry, 54(13), 4855-60.
Rochfort SJ, et al. Characterization of Flavonol Conjugates in Immature Leaves of Pak Choi [Brassica Rapa L. Ssp. Chinensis L. (Hanelt.)] By HPLC-DAD and LC-MS/MS. J Agric Food Chem. 2006 Jun 28;54(13):4855-60. PubMed PMID: 16787039.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of flavonol conjugates in immature leaves of pak choi [Brassica rapa L. Ssp. chinensis L. (Hanelt.)] by HPLC-DAD and LC-MS/MS. AU - Rochfort,Simone J, AU - Imsic,Michael, AU - Jones,Rod, AU - Trenerry,V Craige, AU - Tomkins,Bruce, PY - 2006/6/22/pubmed PY - 2006/8/11/medline PY - 2006/6/22/entrez SP - 4855 EP - 60 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 54 IS - 13 N2 - The flavonoid composition of immature leaves of pak choi [Brassica rapa L. ssp. chinensis L. (Hanelt.)] was investigated. Flavonol aglycone content was measured in 11 pak choi varieties, indicating significant differences (P < 0.05) in content between varieties and relatively high contents of kaempferol and isorhamnetin. Levels of quercetin ranged from 3.2 to 6.1 mg/100 g of dry weight (DW), whereas levels of isorhamnetin and kaempferol were significantly higher (8.1-35.1 and 36.0-102.6 mg/100 g of DW, respectively). A large number of glycoside and hydroxycinnamic acid derivatives of quercetin, kaempferol, and isorhamnetin were identified in cv. 'Shanghai' by LC/UV-DAD/ESI-MS/MS. The UV-DAD data allowed identification of hydroxycinnamic acid derivatives, but detailed MS/MS fragmentations were required for the structure elucidation. Pak choi could be a potentially important source of dietary flavonols, in particular, kaempferol and isorhamnetin. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/16787039/Characterization_of_flavonol_conjugates_in_immature_leaves_of_pak_choi_[Brassica_rapa_L__Ssp__chinensis_L___Hanelt__]_by_HPLC_DAD_and_LC_MS/MS_ L2 - https://doi.org/10.1021/jf060154j DB - PRIME DP - Unbound Medicine ER -