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GAMYB controls different sets of genes and is differentially regulated by microRNA in aleurone cells and anthers.
Plant J. 2006 Aug; 47(3):427-44.PJ

Abstract

GAMYB is a component of gibberellin (GA) signaling in cereal aleurone cells, and has an important role in flower development. However, it is unclear how GAMYB function is regulated. We examined the involvement of a microRNA, miR159, in the regulation of GAMYB expression in cereal aleurone cells and flower development. In aleurone cells, no miR159 expression was observed with or without GA treatment, suggesting that miR159 is not involved in the regulation of GAMYB and GAMYB-like genes in this tissue. miR159 was expressed in tissues other than aleurone, and miR159 over-expressors showed similar but more severe phenotypes than the gamyb mutant. GAMYB and GAMYB-like genes are co-expressed with miR159 in anthers, and the mRNA levels for GAMYB and GAMYB-like genes are negatively correlated with miR159 levels during anther development. Thus, OsGAMYB and OsGAMYB-like genes are regulated by miR159 in flowers. A microarray analysis revealed that OsGAMYB and its upstream regulator SLR1 are involved in the regulation of almost all GA-mediated gene expression in rice aleurone cells. Moreover, different sets of genes are regulated by GAMYB in aleurone cells and anthers. GAMYB binds directly to promoter regions of its target genes in anthers as well as aleurone cells. Based on these observations, we suggest that the regulation of GAMYB expression and GAMYB function are different in aleurone cells and flowers in rice.

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Authors+Show Affiliations

Tsuji H
Bioscience and Biotechnology Center, Nagoya University, Furocho, Chikusa, Nagoya 464-8601, Japan.
Aya K
No affiliation info available
Ueguchi-Tanaka M
No affiliation info available
Shimada Y
No affiliation info available
Nakazono M
No affiliation info available
Watanabe R
No affiliation info available
Nishizawa NK
No affiliation info available
Gomi K
No affiliation info available
Shimada A
No affiliation info available
Kitano H
No affiliation info available
Ashikari M
No affiliation info available
Matsuoka M
No affiliation info available

MeSH

Amino Acid SequenceFlowersGene Expression ProfilingGene Expression Regulation, PlantGenes, PlantGibberellinsMicroRNAsMolecular Sequence DataMutationOligonucleotide Array Sequence AnalysisOryzaPhenotypePlant ProteinsPlants, Genetically ModifiedRNA, MessengerSequence Alignment

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16792694

Citation

Tsuji, Hiroyuki, et al. "GAMYB Controls Different Sets of Genes and Is Differentially Regulated By microRNA in Aleurone Cells and Anthers." The Plant Journal : for Cell and Molecular Biology, vol. 47, no. 3, 2006, pp. 427-44.
Tsuji H, Aya K, Ueguchi-Tanaka M, et al. GAMYB controls different sets of genes and is differentially regulated by microRNA in aleurone cells and anthers. Plant J. 2006;47(3):427-44.
Tsuji, H., Aya, K., Ueguchi-Tanaka, M., Shimada, Y., Nakazono, M., Watanabe, R., Nishizawa, N. K., Gomi, K., Shimada, A., Kitano, H., Ashikari, M., & Matsuoka, M. (2006). GAMYB controls different sets of genes and is differentially regulated by microRNA in aleurone cells and anthers. The Plant Journal : for Cell and Molecular Biology, 47(3), 427-44.
Tsuji H, et al. GAMYB Controls Different Sets of Genes and Is Differentially Regulated By microRNA in Aleurone Cells and Anthers. Plant J. 2006;47(3):427-44. PubMed PMID: 16792694.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - GAMYB controls different sets of genes and is differentially regulated by microRNA in aleurone cells and anthers. AU - Tsuji,Hiroyuki, AU - Aya,Koichiro, AU - Ueguchi-Tanaka,Miyako, AU - Shimada,Yukihisa, AU - Nakazono,Mikio, AU - Watanabe,Ryosuke, AU - Nishizawa,Naoko K, AU - Gomi,Kenji, AU - Shimada,Asako, AU - Kitano,Hidemi, AU - Ashikari,Motoyuki, AU - Matsuoka,Makoto, Y1 - 2006/06/22/ PY - 2006/6/24/pubmed PY - 2006/10/7/medline PY - 2006/6/24/entrez SP - 427 EP - 44 JF - The Plant journal : for cell and molecular biology JO - Plant J VL - 47 IS - 3 N2 - GAMYB is a component of gibberellin (GA) signaling in cereal aleurone cells, and has an important role in flower development. However, it is unclear how GAMYB function is regulated. We examined the involvement of a microRNA, miR159, in the regulation of GAMYB expression in cereal aleurone cells and flower development. In aleurone cells, no miR159 expression was observed with or without GA treatment, suggesting that miR159 is not involved in the regulation of GAMYB and GAMYB-like genes in this tissue. miR159 was expressed in tissues other than aleurone, and miR159 over-expressors showed similar but more severe phenotypes than the gamyb mutant. GAMYB and GAMYB-like genes are co-expressed with miR159 in anthers, and the mRNA levels for GAMYB and GAMYB-like genes are negatively correlated with miR159 levels during anther development. Thus, OsGAMYB and OsGAMYB-like genes are regulated by miR159 in flowers. A microarray analysis revealed that OsGAMYB and its upstream regulator SLR1 are involved in the regulation of almost all GA-mediated gene expression in rice aleurone cells. Moreover, different sets of genes are regulated by GAMYB in aleurone cells and anthers. GAMYB binds directly to promoter regions of its target genes in anthers as well as aleurone cells. Based on these observations, we suggest that the regulation of GAMYB expression and GAMYB function are different in aleurone cells and flowers in rice. SN - 0960-7412 UR - https://www.unboundmedicine.com/medline/citation/16792694/GAMYB_controls_different_sets_of_genes_and_is_differentially_regulated_by_microRNA_in_aleurone_cells_and_anthers_ L2 - https://doi.org/10.1111/j.1365-313X.2006.02795.x DB - PRIME DP - Unbound Medicine ER -