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Insulin-like growth factor-1 (IGF-1) induces the activation/phosphorylation of Akt kinase and cAMP response element-binding protein (CREB) by activating different signaling pathways in PC12 cells.
BMC Neurosci. 2006 Jun 22; 7:51.BN

Abstract

BACKGROUND

Insulin-like growth factor-1 (IGF-1) is a polypeptide growth factor with a variety of functions in both neuronal and non-neuronal cells. IGF-1 plays anti-apoptotic and other functions by activating multiple signaling pathways including Akt kinase, a serine/threonine kinase essential for cell survival. The nuclear transcription factor cAMP response element-binding protein (CREB) may also be involved although relationships between these two proteins in IGF-1 receptor signaling and protection is not clear, especially in neuronal cells.

RESULTS

IGF-1, in a concentration- and time-dependent manner, induces the activation/phosphorylation of Akt and CREB in PC12 cells by activating different signaling pathways. IGF-1 induced a sustained phosphorylation of Akt while only a transient one was seen for CREB. The phosphorylation of Akt is mediated by the PI3 kinase pathway while that of CREB is dependent on the activation of both MAPK kinase and p38 MAPK. Moreover, the stimulation of PKC attenuated the phosphorylation of Akt induced by IGF-1 while enhancing that of CREB. Survival assays with various kinase inhibitors suggested that the activation/phosphorylation of both Akt and CREB contributes to IGF-1 mediated cell survival in PC12 cells.

CONCLUSION

These data suggest that IGF-1 induced the activation of Akt and CREB using distinct pathways in PC12 cells.

Authors+Show Affiliations

Department of Pharmacology, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, China. whzheng123@gmail.comNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16792806

Citation

Zheng, Wen-Hua, and Rémi Quirion. "Insulin-like Growth Factor-1 (IGF-1) Induces the Activation/phosphorylation of Akt Kinase and cAMP Response Element-binding Protein (CREB) By Activating Different Signaling Pathways in PC12 Cells." BMC Neuroscience, vol. 7, 2006, p. 51.
Zheng WH, Quirion R. Insulin-like growth factor-1 (IGF-1) induces the activation/phosphorylation of Akt kinase and cAMP response element-binding protein (CREB) by activating different signaling pathways in PC12 cells. BMC Neurosci. 2006;7:51.
Zheng, W. H., & Quirion, R. (2006). Insulin-like growth factor-1 (IGF-1) induces the activation/phosphorylation of Akt kinase and cAMP response element-binding protein (CREB) by activating different signaling pathways in PC12 cells. BMC Neuroscience, 7, 51.
Zheng WH, Quirion R. Insulin-like Growth Factor-1 (IGF-1) Induces the Activation/phosphorylation of Akt Kinase and cAMP Response Element-binding Protein (CREB) By Activating Different Signaling Pathways in PC12 Cells. BMC Neurosci. 2006 Jun 22;7:51. PubMed PMID: 16792806.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Insulin-like growth factor-1 (IGF-1) induces the activation/phosphorylation of Akt kinase and cAMP response element-binding protein (CREB) by activating different signaling pathways in PC12 cells. AU - Zheng,Wen-Hua, AU - Quirion,Rémi, Y1 - 2006/06/22/ PY - 2005/12/21/received PY - 2006/06/22/accepted PY - 2006/6/24/pubmed PY - 2006/9/21/medline PY - 2006/6/24/entrez SP - 51 EP - 51 JF - BMC neuroscience JO - BMC Neurosci VL - 7 N2 - BACKGROUND: Insulin-like growth factor-1 (IGF-1) is a polypeptide growth factor with a variety of functions in both neuronal and non-neuronal cells. IGF-1 plays anti-apoptotic and other functions by activating multiple signaling pathways including Akt kinase, a serine/threonine kinase essential for cell survival. The nuclear transcription factor cAMP response element-binding protein (CREB) may also be involved although relationships between these two proteins in IGF-1 receptor signaling and protection is not clear, especially in neuronal cells. RESULTS: IGF-1, in a concentration- and time-dependent manner, induces the activation/phosphorylation of Akt and CREB in PC12 cells by activating different signaling pathways. IGF-1 induced a sustained phosphorylation of Akt while only a transient one was seen for CREB. The phosphorylation of Akt is mediated by the PI3 kinase pathway while that of CREB is dependent on the activation of both MAPK kinase and p38 MAPK. Moreover, the stimulation of PKC attenuated the phosphorylation of Akt induced by IGF-1 while enhancing that of CREB. Survival assays with various kinase inhibitors suggested that the activation/phosphorylation of both Akt and CREB contributes to IGF-1 mediated cell survival in PC12 cells. CONCLUSION: These data suggest that IGF-1 induced the activation of Akt and CREB using distinct pathways in PC12 cells. SN - 1471-2202 UR - https://www.unboundmedicine.com/medline/citation/16792806/Insulin_like_growth_factor_1__IGF_1__induces_the_activation/phosphorylation_of_Akt_kinase_and_cAMP_response_element_binding_protein__CREB__by_activating_different_signaling_pathways_in_PC12_cells_ L2 - https://bmcneurosci.biomedcentral.com/articles/10.1186/1471-2202-7-51 DB - PRIME DP - Unbound Medicine ER -