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A simple method to fabricate a chitosan-gold nanoparticles film and its application in glucose biosensor.
Bioelectrochemistry. 2007 May; 70(2):342-7.B

Abstract

A novel film of chitosan-gold nanoparticles is fabricated by a direct and facile electrochemical deposition method and its application in glucose biosensor is investigated. HAuCl(4) solution is mixed with chitosan and electrochemically reduced to gold nanoparticles, which can be stabilized by chitosan and electrodeposited onto glassy carbon electrode surfaces along with the electrodeposition of chitosan. Then a model enzyme, glucose oxidase (GOD) is immobilized onto the resulting film to construct a glucose biosensor through self-assembly. The resulting modified electrode surfaces are characterized with both AFM and cyclic voltammetry. Effects of chitosan and HAuCl(4) concentration in the mixture together with the deposition time and the applied voltage on the amperometric response of the biosensor are also investigated. The linear range of the glucose biosensor is from 5.0 x 10(-5) approximately 1.30 x 10(-3) M with a Michaelis-Menten constant of 3.5 mM and a detection limit of about 13 microM.

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Authors+Show Affiliations

Du Y
Key Lab of Analytical Chemistry for Life Science (MOE), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
Luo XL
No affiliation info available
Xu JJ
No affiliation info available
Chen HY
No affiliation info available

MeSH

Biosensing TechniquesChitosanCrystallizationElectrochemistryEnzymes, ImmobilizedEquipment DesignEquipment Failure AnalysisGlucoseGlucose OxidaseGoldMembranes, ArtificialMicroelectrodesNanoparticlesParticle SizeReproducibility of ResultsSensitivity and Specificity

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16793348

Citation

Du, Ying, et al. "A Simple Method to Fabricate a Chitosan-gold Nanoparticles Film and Its Application in Glucose Biosensor." Bioelectrochemistry (Amsterdam, Netherlands), vol. 70, no. 2, 2007, pp. 342-7.
Du Y, Luo XL, Xu JJ, et al. A simple method to fabricate a chitosan-gold nanoparticles film and its application in glucose biosensor. Bioelectrochemistry. 2007;70(2):342-7.
Du, Y., Luo, X. L., Xu, J. J., & Chen, H. Y. (2007). A simple method to fabricate a chitosan-gold nanoparticles film and its application in glucose biosensor. Bioelectrochemistry (Amsterdam, Netherlands), 70(2), 342-7.
Du Y, et al. A Simple Method to Fabricate a Chitosan-gold Nanoparticles Film and Its Application in Glucose Biosensor. Bioelectrochemistry. 2007;70(2):342-7. PubMed PMID: 16793348.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A simple method to fabricate a chitosan-gold nanoparticles film and its application in glucose biosensor. AU - Du,Ying, AU - Luo,Xi-Liang, AU - Xu,Jing-Juan, AU - Chen,Hong-Yuan, Y1 - 2006/05/16/ PY - 2005/12/11/received PY - 2006/04/12/revised PY - 2006/05/02/accepted PY - 2006/6/24/pubmed PY - 2007/6/27/medline PY - 2006/6/24/entrez SP - 342 EP - 7 JF - Bioelectrochemistry (Amsterdam, Netherlands) JO - Bioelectrochemistry VL - 70 IS - 2 N2 - A novel film of chitosan-gold nanoparticles is fabricated by a direct and facile electrochemical deposition method and its application in glucose biosensor is investigated. HAuCl(4) solution is mixed with chitosan and electrochemically reduced to gold nanoparticles, which can be stabilized by chitosan and electrodeposited onto glassy carbon electrode surfaces along with the electrodeposition of chitosan. Then a model enzyme, glucose oxidase (GOD) is immobilized onto the resulting film to construct a glucose biosensor through self-assembly. The resulting modified electrode surfaces are characterized with both AFM and cyclic voltammetry. Effects of chitosan and HAuCl(4) concentration in the mixture together with the deposition time and the applied voltage on the amperometric response of the biosensor are also investigated. The linear range of the glucose biosensor is from 5.0 x 10(-5) approximately 1.30 x 10(-3) M with a Michaelis-Menten constant of 3.5 mM and a detection limit of about 13 microM. SN - 1567-5394 UR - https://www.unboundmedicine.com/medline/citation/16793348/A_simple_method_to_fabricate_a_chitosan_gold_nanoparticles_film_and_its_application_in_glucose_biosensor_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-5394(06)00078-8 DB - PRIME DP - Unbound Medicine ER -