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Purification and assays of Saccharomyces cerevisiae homologous recombination proteins.
Methods Enzymol. 2006; 408:445-63.ME

Abstract

Homologous recombination is an important means of eliminating DNA double strand breaks from chromosomes. The homologous recombination reaction is mediated by the Rad51 recombinase, which requires a number of ancillary factors for maximal efficiency. The development of purification procedures and biochemical assays for yeast Rad51 and other yeast recombination proteins has allowed investigators to begin dissecting the hierarchy of physical and functional interactions among these protein factors that govern the integrity of the homologous recombination machinery. The biochemical studies done with yeast recombination factors have helped formulate conceptual frameworks to guide similar endeavors in other eukaryotes, including humans. Continuing efforts with reconstituted systems that comprise yeast factors will undoubtedly continue to provide insights into the mechanistic intricacy of the homologous recombination machinery.

Authors+Show Affiliations

Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

16793386

Citation

Van Komen, Stephen, et al. "Purification and Assays of Saccharomyces Cerevisiae Homologous Recombination Proteins." Methods in Enzymology, vol. 408, 2006, pp. 445-63.
Van Komen S, Macris M, Sehorn MG, et al. Purification and assays of Saccharomyces cerevisiae homologous recombination proteins. Methods Enzymol. 2006;408:445-63.
Van Komen, S., Macris, M., Sehorn, M. G., & Sung, P. (2006). Purification and assays of Saccharomyces cerevisiae homologous recombination proteins. Methods in Enzymology, 408, 445-63.
Van Komen S, et al. Purification and Assays of Saccharomyces Cerevisiae Homologous Recombination Proteins. Methods Enzymol. 2006;408:445-63. PubMed PMID: 16793386.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Purification and assays of Saccharomyces cerevisiae homologous recombination proteins. AU - Van Komen,Stephen, AU - Macris,Margaret, AU - Sehorn,Michael G, AU - Sung,Patrick, PY - 2006/6/24/pubmed PY - 2007/2/6/medline PY - 2006/6/24/entrez SP - 445 EP - 63 JF - Methods in enzymology JO - Methods Enzymol VL - 408 N2 - Homologous recombination is an important means of eliminating DNA double strand breaks from chromosomes. The homologous recombination reaction is mediated by the Rad51 recombinase, which requires a number of ancillary factors for maximal efficiency. The development of purification procedures and biochemical assays for yeast Rad51 and other yeast recombination proteins has allowed investigators to begin dissecting the hierarchy of physical and functional interactions among these protein factors that govern the integrity of the homologous recombination machinery. The biochemical studies done with yeast recombination factors have helped formulate conceptual frameworks to guide similar endeavors in other eukaryotes, including humans. Continuing efforts with reconstituted systems that comprise yeast factors will undoubtedly continue to provide insights into the mechanistic intricacy of the homologous recombination machinery. SN - 0076-6879 UR - https://www.unboundmedicine.com/medline/citation/16793386/Purification_and_assays_of_Saccharomyces_cerevisiae_homologous_recombination_proteins_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0076-6879(06)08028-1 DB - PRIME DP - Unbound Medicine ER -