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In vitro endothelial potential of human UC blood-derived mesenchymal stem cells.
Cytotherapy. 2006; 8(3):215-27.C

Abstract

BACKGROUND

Human mesenchymal stem cells (MSC) possess powerful ex vivo expansion and versatile differentiation potential, placing themselves at the forefront of the field of stem cell-based therapy and transplantation. Of high clinical relevance is the endothelial differentiation potential of MSC, which can be used to treat various forms of ischemic vascular disease.

METHODS

We investigated whether human umbilical cord blood (UCB)-derived MSC are able to differentiate in vitro along an endothelial lineage, by using flow cytometry, RT-PCR and immunofluorescence analyzes, as well as an Ab array method.

RESULTS

When the cells were incubated for up to 3 weeks in the presence of VEGF, EGF and hydrocortisone, they began to express a variety of endothelial lineage surface markers, such as Flk-1, Flt-1, VE-Cadherin, vWF, VCAM-1, Tie-1 and Tie-2, and to secrete a specific set of cytokines. Differentiated cells were also found to be able to uptake low-density lipoprotein and form a tubular network structure.

DISCUSSION

These observations have led us to conclude that UCB-derived MSC retain endothelial potential that is suitable for basic and clinical studies aimed at the development of vasculature-directed regenerative medicine.

Authors+Show Affiliations

Research Institute of Biotechnology, Histostem Co., Seoul, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16793731

Citation

Gang, E J., et al. "In Vitro Endothelial Potential of Human UC Blood-derived Mesenchymal Stem Cells." Cytotherapy, vol. 8, no. 3, 2006, pp. 215-27.
Gang EJ, Jeong JA, Han S, et al. In vitro endothelial potential of human UC blood-derived mesenchymal stem cells. Cytotherapy. 2006;8(3):215-27.
Gang, E. J., Jeong, J. A., Han, S., Yan, Q., Jeon, C. J., & Kim, H. (2006). In vitro endothelial potential of human UC blood-derived mesenchymal stem cells. Cytotherapy, 8(3), 215-27.
Gang EJ, et al. In Vitro Endothelial Potential of Human UC Blood-derived Mesenchymal Stem Cells. Cytotherapy. 2006;8(3):215-27. PubMed PMID: 16793731.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro endothelial potential of human UC blood-derived mesenchymal stem cells. AU - Gang,E J, AU - Jeong,J A, AU - Han,S, AU - Yan,Q, AU - Jeon,C-J, AU - Kim,H, PY - 2006/6/24/pubmed PY - 2006/10/25/medline PY - 2006/6/24/entrez SP - 215 EP - 27 JF - Cytotherapy JO - Cytotherapy VL - 8 IS - 3 N2 - BACKGROUND: Human mesenchymal stem cells (MSC) possess powerful ex vivo expansion and versatile differentiation potential, placing themselves at the forefront of the field of stem cell-based therapy and transplantation. Of high clinical relevance is the endothelial differentiation potential of MSC, which can be used to treat various forms of ischemic vascular disease. METHODS: We investigated whether human umbilical cord blood (UCB)-derived MSC are able to differentiate in vitro along an endothelial lineage, by using flow cytometry, RT-PCR and immunofluorescence analyzes, as well as an Ab array method. RESULTS: When the cells were incubated for up to 3 weeks in the presence of VEGF, EGF and hydrocortisone, they began to express a variety of endothelial lineage surface markers, such as Flk-1, Flt-1, VE-Cadherin, vWF, VCAM-1, Tie-1 and Tie-2, and to secrete a specific set of cytokines. Differentiated cells were also found to be able to uptake low-density lipoprotein and form a tubular network structure. DISCUSSION: These observations have led us to conclude that UCB-derived MSC retain endothelial potential that is suitable for basic and clinical studies aimed at the development of vasculature-directed regenerative medicine. SN - 1465-3249 UR - https://www.unboundmedicine.com/medline/citation/16793731/In_vitro_endothelial_potential_of_human_UC_blood_derived_mesenchymal_stem_cells_ DB - PRIME DP - Unbound Medicine ER -