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Protective effect of tanshinone IIA on human umbilical vein endothelial cell injured by hydrogen peroxide and its mechanism.
J Ethnopharmacol. 2006 Nov 24; 108(2):217-22.JE

Abstract

Tanshinone IIA (Tan IIA) is isolated from Salvia miltiorrhiza, the root of which is widely used as a traditional Chinese medicine to treat atherosclerosis. The aim of the present study was to evaluate the putative protective effect of Tan IIA in a human umbilical vein endothelial cell line (ECV-304) injured by hydrogen peroxide in vitro and the mechanism of its protection. The percentage of cell viability was evaluated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay. The endothelial cell apoptosis and expression of cluster of differentiation 40 (CD40) were detected by flow cytometric analysis. Preincubation with Tan IIA significantly increased the viability of ECV-304 cell injured by hydrogen peroxide, which was accompanied with the increased nitric oxide level and superoxide dismutase activity in a dose-dependent manner. Moreover, cell apoptosis and CD40 expression were decreased in a dose-dependent manner. In conclusion, our data suggests that Tan IIA protects ECV-304 cell damage induced by hydrogen peroxide through its anti-oxidant effect and CD40 anti-inflammatory approach.

Authors+Show Affiliations

Department of Pharmacology, Xi'an Jiaotong University School of Medicine, Xi'an, Shaanxi, PR China. linrong@mail.xjtu.edu.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16797899

Citation

Lin, Rong, et al. "Protective Effect of Tanshinone IIA On Human Umbilical Vein Endothelial Cell Injured By Hydrogen Peroxide and Its Mechanism." Journal of Ethnopharmacology, vol. 108, no. 2, 2006, pp. 217-22.
Lin R, Wang WR, Liu JT, et al. Protective effect of tanshinone IIA on human umbilical vein endothelial cell injured by hydrogen peroxide and its mechanism. J Ethnopharmacol. 2006;108(2):217-22.
Lin, R., Wang, W. R., Liu, J. T., Yang, G. D., & Han, C. J. (2006). Protective effect of tanshinone IIA on human umbilical vein endothelial cell injured by hydrogen peroxide and its mechanism. Journal of Ethnopharmacology, 108(2), 217-22.
Lin R, et al. Protective Effect of Tanshinone IIA On Human Umbilical Vein Endothelial Cell Injured By Hydrogen Peroxide and Its Mechanism. J Ethnopharmacol. 2006 Nov 24;108(2):217-22. PubMed PMID: 16797899.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effect of tanshinone IIA on human umbilical vein endothelial cell injured by hydrogen peroxide and its mechanism. AU - Lin,Rong, AU - Wang,Wei-Rong, AU - Liu,Jun-Tian, AU - Yang,Guang-De, AU - Han,Chun-Jie, Y1 - 2006/05/16/ PY - 2006/03/26/received PY - 2006/04/24/revised PY - 2006/05/08/accepted PY - 2006/6/27/pubmed PY - 2007/4/25/medline PY - 2006/6/27/entrez SP - 217 EP - 22 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 108 IS - 2 N2 - Tanshinone IIA (Tan IIA) is isolated from Salvia miltiorrhiza, the root of which is widely used as a traditional Chinese medicine to treat atherosclerosis. The aim of the present study was to evaluate the putative protective effect of Tan IIA in a human umbilical vein endothelial cell line (ECV-304) injured by hydrogen peroxide in vitro and the mechanism of its protection. The percentage of cell viability was evaluated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay. The endothelial cell apoptosis and expression of cluster of differentiation 40 (CD40) were detected by flow cytometric analysis. Preincubation with Tan IIA significantly increased the viability of ECV-304 cell injured by hydrogen peroxide, which was accompanied with the increased nitric oxide level and superoxide dismutase activity in a dose-dependent manner. Moreover, cell apoptosis and CD40 expression were decreased in a dose-dependent manner. In conclusion, our data suggests that Tan IIA protects ECV-304 cell damage induced by hydrogen peroxide through its anti-oxidant effect and CD40 anti-inflammatory approach. SN - 0378-8741 UR - https://www.unboundmedicine.com/medline/citation/16797899/Protective_effect_of_tanshinone_IIA_on_human_umbilical_vein_endothelial_cell_injured_by_hydrogen_peroxide_and_its_mechanism_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(06)00253-4 DB - PRIME DP - Unbound Medicine ER -