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Is interferon gamma suppression after cardiac surgery caused by a decreased interleukin-12 synthesis?
Ann Thorac Surg. 2006 Jul; 82(1):103-9.AT

Abstract

BACKGROUND

The suppression of interferon gamma (IFN-gamma) synthesis after cardiac surgery is discussed as a cause of postoperative immunosuppression that predisposes to postoperative infectious complications. Because several studies have suggested that interleukin-12 (IL-12) production by monocytes and macrophages is reduced after cardiac surgery, this might cause a decrease in IFN-gamma release. To better understand these processes, we assessed the role of IL-12 in IFN-gamma synthesis in vitro before and after cardiac surgery.

METHODS

Heparinized whole blood samples were obtained from 20 patients undergoing elective cardiac surgery preoperatively (day 0) and on the first (day 1), third (day 3), and fifth (day 5) postoperative days, and stimulated (24 hours) with staphylococcal enterotoxin B and lipopolysaccharide. Recombinant IL-12 was added at each time point investigated. Interferon-gamma, IL-12, IL-2, IL-4, and IL-5 concentrations and histocompatibility leukocyte antigen-DR (HLA-DR) expression on monocytes and macrophages were assayed by flow cytometry.

RESULTS

The HLA-DR expression, IL-12 release, and IFN-gamma synthesis were significantly reduced on day 1, day 3, and day 5. Recovery began on day 3. Interleukin-12 caused a significant increase in IFN-gamma synthesis at each time point. When IL-12 was added, IFN-gamma synthesis returned to preoperative levels on days 3 and 5.

CONCLUSIONS

The synthesis of IFN-gamma is significantly reduced after cardiac surgery. The application of IL-12 causes an increase in IFN-gamma synthesis before surgery and a return of IFN-gamma to preoperative levels within a few days after surgery. These findings suggest that postoperative suppression of IFN-gamma release is caused by a decrease in IL-12 synthesis. In addition, IL-12 has a mainly proinflammatory effect both before and after surgery.

Authors+Show Affiliations

Department of Cardiovascular Surgery, Bundeswehr Central Hospital, Koblenz, Germany. dr.axel.franke@t-online.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16798198

Citation

Franke, Axel, et al. "Is Interferon Gamma Suppression After Cardiac Surgery Caused By a Decreased Interleukin-12 Synthesis?" The Annals of Thoracic Surgery, vol. 82, no. 1, 2006, pp. 103-9.
Franke A, Lante W, Kurig E, et al. Is interferon gamma suppression after cardiac surgery caused by a decreased interleukin-12 synthesis? Ann Thorac Surg. 2006;82(1):103-9.
Franke, A., Lante, W., Kurig, E., Zöller, L. G., Weinhold, C., & Markewitz, A. (2006). Is interferon gamma suppression after cardiac surgery caused by a decreased interleukin-12 synthesis? The Annals of Thoracic Surgery, 82(1), 103-9.
Franke A, et al. Is Interferon Gamma Suppression After Cardiac Surgery Caused By a Decreased Interleukin-12 Synthesis. Ann Thorac Surg. 2006;82(1):103-9. PubMed PMID: 16798198.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Is interferon gamma suppression after cardiac surgery caused by a decreased interleukin-12 synthesis? AU - Franke,Axel, AU - Lante,Wolfgang, AU - Kurig,Edmond, AU - Zöller,Lothar G, AU - Weinhold,Christian, AU - Markewitz,Andreas, PY - 2005/11/06/received PY - 2006/02/17/revised PY - 2006/02/22/accepted PY - 2006/6/27/pubmed PY - 2006/8/15/medline PY - 2006/6/27/entrez SP - 103 EP - 9 JF - The Annals of thoracic surgery JO - Ann Thorac Surg VL - 82 IS - 1 N2 - BACKGROUND: The suppression of interferon gamma (IFN-gamma) synthesis after cardiac surgery is discussed as a cause of postoperative immunosuppression that predisposes to postoperative infectious complications. Because several studies have suggested that interleukin-12 (IL-12) production by monocytes and macrophages is reduced after cardiac surgery, this might cause a decrease in IFN-gamma release. To better understand these processes, we assessed the role of IL-12 in IFN-gamma synthesis in vitro before and after cardiac surgery. METHODS: Heparinized whole blood samples were obtained from 20 patients undergoing elective cardiac surgery preoperatively (day 0) and on the first (day 1), third (day 3), and fifth (day 5) postoperative days, and stimulated (24 hours) with staphylococcal enterotoxin B and lipopolysaccharide. Recombinant IL-12 was added at each time point investigated. Interferon-gamma, IL-12, IL-2, IL-4, and IL-5 concentrations and histocompatibility leukocyte antigen-DR (HLA-DR) expression on monocytes and macrophages were assayed by flow cytometry. RESULTS: The HLA-DR expression, IL-12 release, and IFN-gamma synthesis were significantly reduced on day 1, day 3, and day 5. Recovery began on day 3. Interleukin-12 caused a significant increase in IFN-gamma synthesis at each time point. When IL-12 was added, IFN-gamma synthesis returned to preoperative levels on days 3 and 5. CONCLUSIONS: The synthesis of IFN-gamma is significantly reduced after cardiac surgery. The application of IL-12 causes an increase in IFN-gamma synthesis before surgery and a return of IFN-gamma to preoperative levels within a few days after surgery. These findings suggest that postoperative suppression of IFN-gamma release is caused by a decrease in IL-12 synthesis. In addition, IL-12 has a mainly proinflammatory effect both before and after surgery. SN - 1552-6259 UR - https://www.unboundmedicine.com/medline/citation/16798198/Is_interferon_gamma_suppression_after_cardiac_surgery_caused_by_a_decreased_interleukin_12_synthesis L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-4975(06)00384-5 DB - PRIME DP - Unbound Medicine ER -