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Phorbol 12-myristate 13-acetate protects against tumor necrosis factor (TNF)-induced necrotic cell death by modulating the recruitment of TNF receptor 1-associated death domain and receptor-interacting protein into the TNF receptor 1 signaling complex: Implication for the regulatory role of protein kinase C.
Mol Pharmacol. 2006 Sep; 70(3):1099-108.MP

Abstract

Protein kinase C (PKC) triggers cellular signals that regulate proliferation or death in a cell- and stimulus-specific manner. Although previous studies have demonstrated that activation of PKC with phorbol 12-myristate 13-acetate (PMA) protects cells from apoptosis induced by a number of mechanisms, including death receptor ligation, little is known about the effect or mechanism of PMA in the necrotic cell death. Here, we demonstrate that PMA-mediated activation of PKC protects against tumor necrosis factor (TNF)-induced necrosis by disrupting formation of the TNF receptor (TNFR)1 signaling complex. Pretreatment with PMA protected L929 cells from TNF-induced necrotic cell death in a PKC-dependent manner, but it did not protect against DNA-damaging agents, including doxorubicin (Adriamycin) and camptothecin. Analysis of the upstream signaling events affected by PMA revealed that it markedly inhibited the TNF-induced recruitment of TNFR1-associated death domain protein (TRADD) and receptor-interacting protein (RIP) to TNFR1, subsequently inhibiting TNF-induced activation of nuclear factor-kappaB and c-Jun NH2-terminal kinase (JNK). However, JNK inhibitors do not significantly affect TNF-induced necrosis, suggesting that the inhibition of JNK activation by PMA is not part of the antinecrotic mechanism. In addition, PMA acted as an antagonist of TNF-induced reactive oxygen species (ROS) production, thereby suppressing activation of ROS-mediated poly(ADP-ribose)polymerase (PARP), and thus inhibiting necrotic cell death. Furthermore, during TNF-induced necrosis, PARP was significantly activated in wild-type mouse embryonic fibroblast (MEF) cells but not in RIP-/- or TNFR-associated factor 2-/-MEF cells. Taken together, these results suggest that PKC activation ensures effective shutdown of the death receptor-mediated necrotic cell death pathway by modulating formation of the death receptor signaling complex.

Authors+Show Affiliations

Department of Pharmacology, College of Medicine, Chungnam National University, 6 Munhwa-dong, Jung-gu, Daejeon 301-131, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16798936

Citation

Byun, Hee Sun, et al. "Phorbol 12-myristate 13-acetate Protects Against Tumor Necrosis Factor (TNF)-induced Necrotic Cell Death By Modulating the Recruitment of TNF Receptor 1-associated Death Domain and Receptor-interacting Protein Into the TNF Receptor 1 Signaling Complex: Implication for the Regulatory Role of Protein Kinase C." Molecular Pharmacology, vol. 70, no. 3, 2006, pp. 1099-108.
Byun HS, Park KA, Won M, et al. Phorbol 12-myristate 13-acetate protects against tumor necrosis factor (TNF)-induced necrotic cell death by modulating the recruitment of TNF receptor 1-associated death domain and receptor-interacting protein into the TNF receptor 1 signaling complex: Implication for the regulatory role of protein kinase C. Mol Pharmacol. 2006;70(3):1099-108.
Byun, H. S., Park, K. A., Won, M., Yang, K. J., Shin, S., Piao, L., Kwak, J. Y., Lee, Z. W., Park, J., Seok, J. H., Liu, Z. G., & Hur, G. M. (2006). Phorbol 12-myristate 13-acetate protects against tumor necrosis factor (TNF)-induced necrotic cell death by modulating the recruitment of TNF receptor 1-associated death domain and receptor-interacting protein into the TNF receptor 1 signaling complex: Implication for the regulatory role of protein kinase C. Molecular Pharmacology, 70(3), 1099-108.
Byun HS, et al. Phorbol 12-myristate 13-acetate Protects Against Tumor Necrosis Factor (TNF)-induced Necrotic Cell Death By Modulating the Recruitment of TNF Receptor 1-associated Death Domain and Receptor-interacting Protein Into the TNF Receptor 1 Signaling Complex: Implication for the Regulatory Role of Protein Kinase C. Mol Pharmacol. 2006;70(3):1099-108. PubMed PMID: 16798936.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phorbol 12-myristate 13-acetate protects against tumor necrosis factor (TNF)-induced necrotic cell death by modulating the recruitment of TNF receptor 1-associated death domain and receptor-interacting protein into the TNF receptor 1 signaling complex: Implication for the regulatory role of protein kinase C. AU - Byun,Hee Sun, AU - Park,Kyeong Ah, AU - Won,Minho, AU - Yang,Keum-Jin, AU - Shin,Sanghee, AU - Piao,Longzhen, AU - Kwak,Jin Young, AU - Lee,Zee-Won, AU - Park,Jongsun, AU - Seok,Jeong Ho, AU - Liu,Zheng-Gang, AU - Hur,Gang Min, Y1 - 2006/06/23/ PY - 2006/6/27/pubmed PY - 2006/9/29/medline PY - 2006/6/27/entrez SP - 1099 EP - 108 JF - Molecular pharmacology JO - Mol Pharmacol VL - 70 IS - 3 N2 - Protein kinase C (PKC) triggers cellular signals that regulate proliferation or death in a cell- and stimulus-specific manner. Although previous studies have demonstrated that activation of PKC with phorbol 12-myristate 13-acetate (PMA) protects cells from apoptosis induced by a number of mechanisms, including death receptor ligation, little is known about the effect or mechanism of PMA in the necrotic cell death. Here, we demonstrate that PMA-mediated activation of PKC protects against tumor necrosis factor (TNF)-induced necrosis by disrupting formation of the TNF receptor (TNFR)1 signaling complex. Pretreatment with PMA protected L929 cells from TNF-induced necrotic cell death in a PKC-dependent manner, but it did not protect against DNA-damaging agents, including doxorubicin (Adriamycin) and camptothecin. Analysis of the upstream signaling events affected by PMA revealed that it markedly inhibited the TNF-induced recruitment of TNFR1-associated death domain protein (TRADD) and receptor-interacting protein (RIP) to TNFR1, subsequently inhibiting TNF-induced activation of nuclear factor-kappaB and c-Jun NH2-terminal kinase (JNK). However, JNK inhibitors do not significantly affect TNF-induced necrosis, suggesting that the inhibition of JNK activation by PMA is not part of the antinecrotic mechanism. In addition, PMA acted as an antagonist of TNF-induced reactive oxygen species (ROS) production, thereby suppressing activation of ROS-mediated poly(ADP-ribose)polymerase (PARP), and thus inhibiting necrotic cell death. Furthermore, during TNF-induced necrosis, PARP was significantly activated in wild-type mouse embryonic fibroblast (MEF) cells but not in RIP-/- or TNFR-associated factor 2-/-MEF cells. Taken together, these results suggest that PKC activation ensures effective shutdown of the death receptor-mediated necrotic cell death pathway by modulating formation of the death receptor signaling complex. SN - 0026-895X UR - https://www.unboundmedicine.com/medline/citation/16798936/Phorbol_12_myristate_13_acetate_protects_against_tumor_necrosis_factor__TNF__induced_necrotic_cell_death_by_modulating_the_recruitment_of_TNF_receptor_1_associated_death_domain_and_receptor_interacting_protein_into_the_TNF_receptor_1_signaling_complex:_Implication_for_the_regulatory_role_of_protein_kinase_C_ L2 - http://molpharm.aspetjournals.org/cgi/pmidlookup?view=long&pmid=16798936 DB - PRIME DP - Unbound Medicine ER -