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Synergism between RPBF Dof and RISBZ1 bZIP activators in the regulation of rice seed expression genes.
Plant Physiol. 2006 Aug; 141(4):1694-707.PP

Abstract

The Dof (DNA binding with one finger) transcriptional activator rice (Oryza sativa) prolamin box binding factor (RPBF), which is involved in gene regulation of rice seed storage proteins, has been isolated from rice cDNA expressed sequence tag clones containing the conserved Dof. RPBF is found as a single gene per haploid genome. Comparison of RPBF genomic and cDNA sequences revealed that the genomic copy is interrupted by one long intron of 1,892 bp in the 5' noncoding region. We demonstrated by transient expression in rice callus protoplasts that the isolated RPBF trans-activated several storage protein genes via an AAAG target sequence located within their promoters, and with methylation interference experiments the additional AAAG-like sequences in promoters of genes expressed in maturing seeds were recognized by the RPBF protein. Binding was sequence specific, since mutation of the AAAG motif or its derivatives decreased both binding and trans-activation by RPBF. Synergism between RPBF and RISBZ1 recognizing the GCN4 motif [TGA(G/C)TCA] was observed in the expression of many storage protein genes. Overexpression of both transcription factors gave rise to much higher levels of expression than the sum of individual activities elicited by either RPBF or RISBZ1 alone. Furthermore, mutation of recognition sites suppressed reciprocal trans-activation ability, indicating that there are mutual interactions between RISBZ1 and RPBF. The RPBF gene is predominantly expressed in maturing endosperm and coordinately expressed with seed storage protein genes, and is involved in the quantitative regulation of genes expressed in the endosperm in cooperation with RISBZ1.

Authors+Show Affiliations

Transgenic Crop Research and Development Center, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16798940

Citation

Yamamoto, Masayuki P., et al. "Synergism Between RPBF Dof and RISBZ1 bZIP Activators in the Regulation of Rice Seed Expression Genes." Plant Physiology, vol. 141, no. 4, 2006, pp. 1694-707.
Yamamoto MP, Onodera Y, Touno SM, et al. Synergism between RPBF Dof and RISBZ1 bZIP activators in the regulation of rice seed expression genes. Plant Physiol. 2006;141(4):1694-707.
Yamamoto, M. P., Onodera, Y., Touno, S. M., & Takaiwa, F. (2006). Synergism between RPBF Dof and RISBZ1 bZIP activators in the regulation of rice seed expression genes. Plant Physiology, 141(4), 1694-707.
Yamamoto MP, et al. Synergism Between RPBF Dof and RISBZ1 bZIP Activators in the Regulation of Rice Seed Expression Genes. Plant Physiol. 2006;141(4):1694-707. PubMed PMID: 16798940.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Synergism between RPBF Dof and RISBZ1 bZIP activators in the regulation of rice seed expression genes. AU - Yamamoto,Masayuki P, AU - Onodera,Yasuyuki, AU - Touno,Satoru M, AU - Takaiwa,Fumio, Y1 - 2006/06/23/ PY - 2006/6/27/pubmed PY - 2006/11/15/medline PY - 2006/6/27/entrez SP - 1694 EP - 707 JF - Plant physiology JO - Plant Physiol. VL - 141 IS - 4 N2 - The Dof (DNA binding with one finger) transcriptional activator rice (Oryza sativa) prolamin box binding factor (RPBF), which is involved in gene regulation of rice seed storage proteins, has been isolated from rice cDNA expressed sequence tag clones containing the conserved Dof. RPBF is found as a single gene per haploid genome. Comparison of RPBF genomic and cDNA sequences revealed that the genomic copy is interrupted by one long intron of 1,892 bp in the 5' noncoding region. We demonstrated by transient expression in rice callus protoplasts that the isolated RPBF trans-activated several storage protein genes via an AAAG target sequence located within their promoters, and with methylation interference experiments the additional AAAG-like sequences in promoters of genes expressed in maturing seeds were recognized by the RPBF protein. Binding was sequence specific, since mutation of the AAAG motif or its derivatives decreased both binding and trans-activation by RPBF. Synergism between RPBF and RISBZ1 recognizing the GCN4 motif [TGA(G/C)TCA] was observed in the expression of many storage protein genes. Overexpression of both transcription factors gave rise to much higher levels of expression than the sum of individual activities elicited by either RPBF or RISBZ1 alone. Furthermore, mutation of recognition sites suppressed reciprocal trans-activation ability, indicating that there are mutual interactions between RISBZ1 and RPBF. The RPBF gene is predominantly expressed in maturing endosperm and coordinately expressed with seed storage protein genes, and is involved in the quantitative regulation of genes expressed in the endosperm in cooperation with RISBZ1. SN - 0032-0889 UR - https://www.unboundmedicine.com/medline/citation/16798940/Synergism_between_RPBF_Dof_and_RISBZ1_bZIP_activators_in_the_regulation_of_rice_seed_expression_genes_ L2 - http://www.plantphysiol.org/cgi/pmidlookup?view=long&pmid=16798940 DB - PRIME DP - Unbound Medicine ER -