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Quantitation of cotinine in nonsmoker saliva using chip-based nanoelectrospray tandem mass spectrometry.
J Anal Toxicol. 2006 Apr; 30(3):178-86.JA

Abstract

A new analytical procedure was developed for the quantitation of nonsmoker salivary cotinine. Small volumes of saliva were diluted with water, fortified with cotinine-d3 (internal standard), then passed through small extraction columns. The analyte and internal standard were eluted with 0.1% (v/v) acetic acid/acetonitrile. Aliquots of each extract were analyzed directly, without chromatographic separation, using chip-based (NanoMate) nanospray tandem mass spectrometry. The calculated detection limit was 0.49 ng cotinine/mL saliva. This method was used to quantify salivary cotinine collected from nonsmoking human subjects living in one of three environmental tobacco smoke (ETS) exposure categories or "cells": 1. smoking home/smoking workplace; 2. smoking home/nonsmoking workplace; and 3. nonsmoking home/smoking workplace. Samples were collected during five sequential days, including Saturday, as part of a larger study to evaluate potential variability in exposure to ETS. Salivary cotinine measurements were made for the purpose of excluding misclassified smokers and for comparison with known levels of exposure to airborne nicotine in each exposure category. The concentrations observed were consistent with those reported from other large studies reported elsewhere. A non-parametric statistical test was applied to the data within each cell. No statistically significant differences were found between the mean cotinine concentrations collected on a weekday as compared to those collected on a weekend day. When the non-parametric test was applied to the three cells, a statistically significant difference was observed between cell 1 compared to cells 2 and 3. The salivary cotinine concentrations were thus statistically invariant over a five-day exposure period, and they were greatest under the conditions of smoking home and smoking workplace.

Authors+Show Affiliations

Organic and Biological Mass Spectrometry Group, Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831-6120, USA. tomkinsba@ornl.govNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

16803652

Citation

Tomkins, Bruce A., et al. "Quantitation of Cotinine in Nonsmoker Saliva Using Chip-based Nanoelectrospray Tandem Mass Spectrometry." Journal of Analytical Toxicology, vol. 30, no. 3, 2006, pp. 178-86.
Tomkins BA, Van Berkel GJ, Jenkins RA, et al. Quantitation of cotinine in nonsmoker saliva using chip-based nanoelectrospray tandem mass spectrometry. J Anal Toxicol. 2006;30(3):178-86.
Tomkins, B. A., Van Berkel, G. J., Jenkins, R. A., & Counts, R. W. (2006). Quantitation of cotinine in nonsmoker saliva using chip-based nanoelectrospray tandem mass spectrometry. Journal of Analytical Toxicology, 30(3), 178-86.
Tomkins BA, et al. Quantitation of Cotinine in Nonsmoker Saliva Using Chip-based Nanoelectrospray Tandem Mass Spectrometry. J Anal Toxicol. 2006;30(3):178-86. PubMed PMID: 16803652.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitation of cotinine in nonsmoker saliva using chip-based nanoelectrospray tandem mass spectrometry. AU - Tomkins,Bruce A, AU - Van Berkel,Gary J, AU - Jenkins,Roger A, AU - Counts,Richard W, PY - 2006/6/29/pubmed PY - 2006/8/19/medline PY - 2006/6/29/entrez SP - 178 EP - 86 JF - Journal of analytical toxicology JO - J Anal Toxicol VL - 30 IS - 3 N2 - A new analytical procedure was developed for the quantitation of nonsmoker salivary cotinine. Small volumes of saliva were diluted with water, fortified with cotinine-d3 (internal standard), then passed through small extraction columns. The analyte and internal standard were eluted with 0.1% (v/v) acetic acid/acetonitrile. Aliquots of each extract were analyzed directly, without chromatographic separation, using chip-based (NanoMate) nanospray tandem mass spectrometry. The calculated detection limit was 0.49 ng cotinine/mL saliva. This method was used to quantify salivary cotinine collected from nonsmoking human subjects living in one of three environmental tobacco smoke (ETS) exposure categories or "cells": 1. smoking home/smoking workplace; 2. smoking home/nonsmoking workplace; and 3. nonsmoking home/smoking workplace. Samples were collected during five sequential days, including Saturday, as part of a larger study to evaluate potential variability in exposure to ETS. Salivary cotinine measurements were made for the purpose of excluding misclassified smokers and for comparison with known levels of exposure to airborne nicotine in each exposure category. The concentrations observed were consistent with those reported from other large studies reported elsewhere. A non-parametric statistical test was applied to the data within each cell. No statistically significant differences were found between the mean cotinine concentrations collected on a weekday as compared to those collected on a weekend day. When the non-parametric test was applied to the three cells, a statistically significant difference was observed between cell 1 compared to cells 2 and 3. The salivary cotinine concentrations were thus statistically invariant over a five-day exposure period, and they were greatest under the conditions of smoking home and smoking workplace. SN - 0146-4760 UR - https://www.unboundmedicine.com/medline/citation/16803652/Quantitation_of_cotinine_in_nonsmoker_saliva_using_chip_based_nanoelectrospray_tandem_mass_spectrometry_ L2 - https://academic.oup.com/jat/article-lookup/doi/10.1093/jat/30.3.178 DB - PRIME DP - Unbound Medicine ER -