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High-performance liquid chromatographic-mass spectrometric determination of methamphetamine and amphetamine enantiomers, desmethylselegiline and selegiline, in hair samples of long-term methamphetamine abusers or selegiline users.
J Anal Toxicol. 2006 May; 30(4):232-7.JA

Abstract

We devised a highly sensitive method for simultaneously determining methamphetamine (MA) and amphetamine (AP) enantiomers, desmethylselegiline (DMSG) and selegiline (SG), in human hair using a derivatization technique and high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS). MA and AP enantiomers and DMSG were effectively converted to trifluoroacetic acid (TFA) derivatives, and the sensitivity of MA and DMSG increased five times over compared with that of free bases. The TFA derivatives of each compound were stable within one week in a stock solution of methanol or for 24 h in the HPLC mobile phase (mixture of methanol and ammonium formate buffer). Each compound was well separated, and calibration curves were linear in the concentration range 0.04-40 ng/mg for MA enantiomers, SG and DMSG, and 0.2-40 ng/mg for AP enantiomers. The accuracy and precision of the method were evaluated, and relative standard deviations were within 7%. Our method was successfully applied to hair samples obtained from long-term MA abusers and SG users. (+)-MA and (+)-AP were detected from three MA abusers at concentrations of 0.79-20.85 and 0.04-3.30 ng/mg, respectively. On the other hand, (-)-MA, (-)-AP, DMSG, and SG were detected in three SG users at concentrations of 2.48-9.05, 0.72-3.10, 0.12-0.59, and 0-0.04 ng/mg, respectively. Based on our obtained data, discrimination of MA abusers from SG users was considered to be possible by comparing optical isomers of MA and AP, the existence of DMSG and/or SG, and the concentration ratio of AP to MA in hair samples.

Authors+Show Affiliations

Criminal Investigation Laboratory, Oita Prefecture Police Headquarters, Oita 870-8502, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

16803660

Citation

Nishida, Kenichi, et al. "High-performance Liquid Chromatographic-mass Spectrometric Determination of Methamphetamine and Amphetamine Enantiomers, Desmethylselegiline and Selegiline, in Hair Samples of Long-term Methamphetamine Abusers or Selegiline Users." Journal of Analytical Toxicology, vol. 30, no. 4, 2006, pp. 232-7.
Nishida K, Itoh S, Inoue N, et al. High-performance liquid chromatographic-mass spectrometric determination of methamphetamine and amphetamine enantiomers, desmethylselegiline and selegiline, in hair samples of long-term methamphetamine abusers or selegiline users. J Anal Toxicol. 2006;30(4):232-7.
Nishida, K., Itoh, S., Inoue, N., Kudo, K., & Ikeda, N. (2006). High-performance liquid chromatographic-mass spectrometric determination of methamphetamine and amphetamine enantiomers, desmethylselegiline and selegiline, in hair samples of long-term methamphetamine abusers or selegiline users. Journal of Analytical Toxicology, 30(4), 232-7.
Nishida K, et al. High-performance Liquid Chromatographic-mass Spectrometric Determination of Methamphetamine and Amphetamine Enantiomers, Desmethylselegiline and Selegiline, in Hair Samples of Long-term Methamphetamine Abusers or Selegiline Users. J Anal Toxicol. 2006;30(4):232-7. PubMed PMID: 16803660.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High-performance liquid chromatographic-mass spectrometric determination of methamphetamine and amphetamine enantiomers, desmethylselegiline and selegiline, in hair samples of long-term methamphetamine abusers or selegiline users. AU - Nishida,Kenichi, AU - Itoh,Shinzi, AU - Inoue,Naohide, AU - Kudo,Keiko, AU - Ikeda,Noriaki, PY - 2006/6/29/pubmed PY - 2006/8/19/medline PY - 2006/6/29/entrez SP - 232 EP - 7 JF - Journal of analytical toxicology JO - J Anal Toxicol VL - 30 IS - 4 N2 - We devised a highly sensitive method for simultaneously determining methamphetamine (MA) and amphetamine (AP) enantiomers, desmethylselegiline (DMSG) and selegiline (SG), in human hair using a derivatization technique and high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS). MA and AP enantiomers and DMSG were effectively converted to trifluoroacetic acid (TFA) derivatives, and the sensitivity of MA and DMSG increased five times over compared with that of free bases. The TFA derivatives of each compound were stable within one week in a stock solution of methanol or for 24 h in the HPLC mobile phase (mixture of methanol and ammonium formate buffer). Each compound was well separated, and calibration curves were linear in the concentration range 0.04-40 ng/mg for MA enantiomers, SG and DMSG, and 0.2-40 ng/mg for AP enantiomers. The accuracy and precision of the method were evaluated, and relative standard deviations were within 7%. Our method was successfully applied to hair samples obtained from long-term MA abusers and SG users. (+)-MA and (+)-AP were detected from three MA abusers at concentrations of 0.79-20.85 and 0.04-3.30 ng/mg, respectively. On the other hand, (-)-MA, (-)-AP, DMSG, and SG were detected in three SG users at concentrations of 2.48-9.05, 0.72-3.10, 0.12-0.59, and 0-0.04 ng/mg, respectively. Based on our obtained data, discrimination of MA abusers from SG users was considered to be possible by comparing optical isomers of MA and AP, the existence of DMSG and/or SG, and the concentration ratio of AP to MA in hair samples. SN - 0146-4760 UR - https://www.unboundmedicine.com/medline/citation/16803660/High_performance_liquid_chromatographic_mass_spectrometric_determination_of_methamphetamine_and_amphetamine_enantiomers_desmethylselegiline_and_selegiline_in_hair_samples_of_long_term_methamphetamine_abusers_or_selegiline_users_ L2 - https://academic.oup.com/jat/article-lookup/doi/10.1093/jat/30.4.232 DB - PRIME DP - Unbound Medicine ER -