Tags

Type your tag names separated by a space and hit enter

Influence of human Ect2 depletion and overexpression on cleavage furrow formation and abscission.
J Cell Sci. 2006 Jul 15; 119(Pt 14):3008-19.JC

Abstract

The guanine nucleotide-exchange factor (GEF) Ect2 is essential for cytokinesis. Here we studied the subcellular localization of Ect2 and examined the consequences of either depleting or overexpressing Ect2 in human cells. We show that in mitotic cells Ect2 localizes to the central spindle and to the cell cortex. The latter association is mediated through a PH domain in Ect2 and central spindle localization requires the MKlp1-MgcRacGAP and MKlp2-Aurora-B complexes. Ect2 directly interacts with MKlp1-MgcRacGAP through its BRCT domain, whereas MKlp2-Aurora-B probably exerts a regulatory role in Ect2 central spindle targeting. Depletion of Ect2 impaired cleavage furrow formation and RhoA and Citron kinase failed to accumulate at the cleavage furrow. Ect2 displacement from the central spindle revealed that physiological levels of this protein in this location are not crucial for RhoA activation and cytokinesis. In cells overexpressing appropriate N-terminal Ect2 fragments, RhoA and Citron kinase localized to the cleavage furrow and ingression occurred, but abscission failed. This failure could be correlated with the persistence of these fragments at structures surrounding the midbody, suggesting that abscission requires the displacement of Ect2 from the contractile ring and its re-import into the nucleus.

Authors+Show Affiliations

Max Planck Institute for Biochemistry, Department of Cell Biology, Am Klopferspitz 18, 82152 Martinsried, Germany.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16803869

Citation

Chalamalasetty, Ravindra B., et al. "Influence of Human Ect2 Depletion and Overexpression On Cleavage Furrow Formation and Abscission." Journal of Cell Science, vol. 119, no. Pt 14, 2006, pp. 3008-19.
Chalamalasetty RB, Hümmer S, Nigg EA, et al. Influence of human Ect2 depletion and overexpression on cleavage furrow formation and abscission. J Cell Sci. 2006;119(Pt 14):3008-19.
Chalamalasetty, R. B., Hümmer, S., Nigg, E. A., & Silljé, H. H. (2006). Influence of human Ect2 depletion and overexpression on cleavage furrow formation and abscission. Journal of Cell Science, 119(Pt 14), 3008-19.
Chalamalasetty RB, et al. Influence of Human Ect2 Depletion and Overexpression On Cleavage Furrow Formation and Abscission. J Cell Sci. 2006 Jul 15;119(Pt 14):3008-19. PubMed PMID: 16803869.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Influence of human Ect2 depletion and overexpression on cleavage furrow formation and abscission. AU - Chalamalasetty,Ravindra B, AU - Hümmer,Stefan, AU - Nigg,Erich A, AU - Silljé,Herman H W, Y1 - 2006/06/27/ PY - 2006/6/29/pubmed PY - 2006/12/9/medline PY - 2006/6/29/entrez SP - 3008 EP - 19 JF - Journal of cell science JO - J. Cell. Sci. VL - 119 IS - Pt 14 N2 - The guanine nucleotide-exchange factor (GEF) Ect2 is essential for cytokinesis. Here we studied the subcellular localization of Ect2 and examined the consequences of either depleting or overexpressing Ect2 in human cells. We show that in mitotic cells Ect2 localizes to the central spindle and to the cell cortex. The latter association is mediated through a PH domain in Ect2 and central spindle localization requires the MKlp1-MgcRacGAP and MKlp2-Aurora-B complexes. Ect2 directly interacts with MKlp1-MgcRacGAP through its BRCT domain, whereas MKlp2-Aurora-B probably exerts a regulatory role in Ect2 central spindle targeting. Depletion of Ect2 impaired cleavage furrow formation and RhoA and Citron kinase failed to accumulate at the cleavage furrow. Ect2 displacement from the central spindle revealed that physiological levels of this protein in this location are not crucial for RhoA activation and cytokinesis. In cells overexpressing appropriate N-terminal Ect2 fragments, RhoA and Citron kinase localized to the cleavage furrow and ingression occurred, but abscission failed. This failure could be correlated with the persistence of these fragments at structures surrounding the midbody, suggesting that abscission requires the displacement of Ect2 from the contractile ring and its re-import into the nucleus. SN - 0021-9533 UR - https://www.unboundmedicine.com/medline/citation/16803869/Influence_of_human_Ect2_depletion_and_overexpression_on_cleavage_furrow_formation_and_abscission_ L2 - http://jcs.biologists.org/cgi/pmidlookup?view=long&pmid=16803869 DB - PRIME DP - Unbound Medicine ER -