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Building of an immunosensor: how can the composition and structure of the thiol attachment layer affect the immunosensor efficiency?
Biosens Bioelectron. 2006 Sep 15; 22(3):440-8.BB

Abstract

Immunosensors, based on the immobilization of a model rabbit antibody on mixed self-assembled monolayers and Protein A as a linking agent on gold transducers, were elaborated and characterized at each step by modulated polarization-infrared spectroscopy (PM-IRRAS) and occasionally by atomic force microscopy (AFM) and quartz crystal microbalance (QCM). By testing two different mixed SAMs comprising 11-mercaptoundecanoic acid (MUA), together with either decanethiol (C9CH3) or mercaptohexanol (C6OH), the role of the chemical composition and structure of the antibody attachment layer upon the sensor performance was demonstrated.

Authors+Show Affiliations

Briand E
Laboratoire de Réactivité de Surface, UMR CNRS 7609, Université Pierre et Marie Curie, 4 Place Jussieu, 75252 Paris Cedex 05, France.
Salmain M
No affiliation info available
Herry JM
No affiliation info available
Perrot H
No affiliation info available
Compère C
No affiliation info available
Pradier CM
No affiliation info available

MeSH

AntibodiesBiosensing TechniquesCross-Linking ReagentsEquipment DesignEquipment Failure AnalysisImmunoassayImmunoglobulin GReproducibility of ResultsSensitivity and SpecificityStaphylococcal Protein ASulfhydryl Compounds

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16806887

Citation

Briand, Elisabeth, et al. "Building of an Immunosensor: How Can the Composition and Structure of the Thiol Attachment Layer Affect the Immunosensor Efficiency?" Biosensors & Bioelectronics, vol. 22, no. 3, 2006, pp. 440-8.
Briand E, Salmain M, Herry JM, et al. Building of an immunosensor: how can the composition and structure of the thiol attachment layer affect the immunosensor efficiency? Biosens Bioelectron. 2006;22(3):440-8.
Briand, E., Salmain, M., Herry, J. M., Perrot, H., Compère, C., & Pradier, C. M. (2006). Building of an immunosensor: how can the composition and structure of the thiol attachment layer affect the immunosensor efficiency? Biosensors & Bioelectronics, 22(3), 440-8.
Briand E, et al. Building of an Immunosensor: How Can the Composition and Structure of the Thiol Attachment Layer Affect the Immunosensor Efficiency. Biosens Bioelectron. 2006 Sep 15;22(3):440-8. PubMed PMID: 16806887.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Building of an immunosensor: how can the composition and structure of the thiol attachment layer affect the immunosensor efficiency? AU - Briand,Elisabeth, AU - Salmain,Michèle, AU - Herry,Jean-Marie, AU - Perrot,Hubert, AU - Compère,Chantal, AU - Pradier,Claire-Marie, Y1 - 2006/06/27/ PY - 2005/09/27/received PY - 2006/03/08/revised PY - 2006/05/05/accepted PY - 2006/6/30/pubmed PY - 2007/1/25/medline PY - 2006/6/30/entrez SP - 440 EP - 8 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 22 IS - 3 N2 - Immunosensors, based on the immobilization of a model rabbit antibody on mixed self-assembled monolayers and Protein A as a linking agent on gold transducers, were elaborated and characterized at each step by modulated polarization-infrared spectroscopy (PM-IRRAS) and occasionally by atomic force microscopy (AFM) and quartz crystal microbalance (QCM). By testing two different mixed SAMs comprising 11-mercaptoundecanoic acid (MUA), together with either decanethiol (C9CH3) or mercaptohexanol (C6OH), the role of the chemical composition and structure of the antibody attachment layer upon the sensor performance was demonstrated. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/16806887/Building_of_an_immunosensor:_how_can_the_composition_and_structure_of_the_thiol_attachment_layer_affect_the_immunosensor_efficiency DB - PRIME DP - Unbound Medicine ER -