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Determination of creatine in commercial creatine powder with new potentiometric and amperometric biosensors.
Artif Cells Blood Substit Immobil Biotechnol. 2006; 34(3):337-47.AC

Abstract

New potentiometric and amperometric biosensors were developed for the determination of creatine. The potentiometric creatine biosensor was prepared by immobilizing urease and creatinase on poly(vinylchloride) (PVC) ammonium membrane electrode containing palmitic acid prepared by using nonactine as an ammonium-ionophore. The linear working range of the biosensor was 1.0 x 10(-5) - 1.0 x 10(-3) M and the response time was about 60 s. The optimum pH, temperature, and buffer concentration were found to be 7.0, 20 degrees C, and 5 mM, respectively. The slope of the electrode was 49.2 mV/p[creatine]. The storage stabilization of the biosensor was investigated and 40-45% decrease in the response was detected after 2 months. The amperometric creatine biosensor was prepared by immobilizing creatinase (CI) and sarcosine oxidase (SO) in a poly(vinylferrocenium) matrix onto the surface of a platinum working electrode by crosslinking with glutaraldehyde (GA) and bovine serum albumine (BSA). Determination of creatine was performed by the oxidation of enzymatically generated H2O2 at +0.7 V vs. Ag/AgCl. The linear working range of the biosensor was 2.0 x 10(-5) - 3.2 x 10(-4) M and the response time was about 50 s. The effects of pH, temperature, enzyme ratio and buffer concentration were investigated and optimum parameters were found to be 7.5, 37 degrees C, 2.5:1 (CI:SO) and 0.05 M, respectively. The determination of creatine in commercial creatine powder was successfully carried out with these creatine biosensors by using the standard addition and calibration curve methods. The results were in good agreement with those obtained from Jaffé method at 95% confidence level.

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Authors+Show Affiliations

Karakuş E
Department of Chemistry, Faculty of Science, Ankara University, Ankara, Turkey. ziyan@science.ankara.edu.tr
Erden PE
No affiliation info available
Pekyardimci S
No affiliation info available
Kiliç E
No affiliation info available

MeSH

Biosensing TechniquesCreatineElectrochemistryEnzymes, ImmobilizedHydrogen-Ion ConcentrationPotentiometryPowdersSarcosine OxidaseTemperatureUreaseUreohydrolases

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16809134

Citation

Karakuş, Emine, et al. "Determination of Creatine in Commercial Creatine Powder With New Potentiometric and Amperometric Biosensors." Artificial Cells, Blood Substitutes, and Immobilization Biotechnology, vol. 34, no. 3, 2006, pp. 337-47.
Karakuş E, Erden PE, Pekyardimci S, et al. Determination of creatine in commercial creatine powder with new potentiometric and amperometric biosensors. Artif Cells Blood Substit Immobil Biotechnol. 2006;34(3):337-47.
Karakuş, E., Erden, P. E., Pekyardimci, S., & Kiliç, E. (2006). Determination of creatine in commercial creatine powder with new potentiometric and amperometric biosensors. Artificial Cells, Blood Substitutes, and Immobilization Biotechnology, 34(3), 337-47.
Karakuş E, et al. Determination of Creatine in Commercial Creatine Powder With New Potentiometric and Amperometric Biosensors. Artif Cells Blood Substit Immobil Biotechnol. 2006;34(3):337-47. PubMed PMID: 16809134.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of creatine in commercial creatine powder with new potentiometric and amperometric biosensors. AU - Karakuş,Emine, AU - Erden,Pinar Esra, AU - Pekyardimci,Sule, AU - Kiliç,Esma, PY - 2006/7/1/pubmed PY - 2006/7/28/medline PY - 2006/7/1/entrez SP - 337 EP - 47 JF - Artificial cells, blood substitutes, and immobilization biotechnology JO - Artif Cells Blood Substit Immobil Biotechnol VL - 34 IS - 3 N2 - New potentiometric and amperometric biosensors were developed for the determination of creatine. The potentiometric creatine biosensor was prepared by immobilizing urease and creatinase on poly(vinylchloride) (PVC) ammonium membrane electrode containing palmitic acid prepared by using nonactine as an ammonium-ionophore. The linear working range of the biosensor was 1.0 x 10(-5) - 1.0 x 10(-3) M and the response time was about 60 s. The optimum pH, temperature, and buffer concentration were found to be 7.0, 20 degrees C, and 5 mM, respectively. The slope of the electrode was 49.2 mV/p[creatine]. The storage stabilization of the biosensor was investigated and 40-45% decrease in the response was detected after 2 months. The amperometric creatine biosensor was prepared by immobilizing creatinase (CI) and sarcosine oxidase (SO) in a poly(vinylferrocenium) matrix onto the surface of a platinum working electrode by crosslinking with glutaraldehyde (GA) and bovine serum albumine (BSA). Determination of creatine was performed by the oxidation of enzymatically generated H2O2 at +0.7 V vs. Ag/AgCl. The linear working range of the biosensor was 2.0 x 10(-5) - 3.2 x 10(-4) M and the response time was about 50 s. The effects of pH, temperature, enzyme ratio and buffer concentration were investigated and optimum parameters were found to be 7.5, 37 degrees C, 2.5:1 (CI:SO) and 0.05 M, respectively. The determination of creatine in commercial creatine powder was successfully carried out with these creatine biosensors by using the standard addition and calibration curve methods. The results were in good agreement with those obtained from Jaffé method at 95% confidence level. SN - 1073-1199 UR - https://www.unboundmedicine.com/medline/citation/16809134/Determination_of_creatine_in_commercial_creatine_powder_with_new_potentiometric_and_amperometric_biosensors_ L2 - https://www.tandfonline.com/doi/full/10.1080/10731190600683985 DB - PRIME DP - Unbound Medicine ER -