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Single amino acid substitutions in the hemagglutinin of influenza A/Singapore/21/04 (H3N2) increase virus growth in embryonated chicken eggs.
Vaccine. 2006 Nov 10; 24(44-46):6691-3.V

Abstract

Most of the recently circulating H3N2 influenza A strains do not replicate well in embryonated chicken eggs and had to be isolated by cell culture, which presents a great challenge for influenza vaccine production using embryonated chicken eggs. We previously reported that a human H3N2 virus, A/Fujian/411/02, which replicates poorly in eggs, could be improved by changing a minimum of two HA residues (G186V/V226I or H183L/V226A). Here, we extended our work to the A/Singapore/21/04 strain that was also unable to grow in eggs. We showed that a single amino acid substitution of either G186V or A196T in the HA resulted in significantly increased virus replication in eggs without affecting virus antigenicity.

Authors+Show Affiliations

MedImmune Vaccines, Inc., 297N. Bernardo Avenue, Mountain View, CA 94043, USA. lub@medimmune.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16814431

Citation

Lu, Bin, et al. "Single Amino Acid Substitutions in the Hemagglutinin of Influenza A/Singapore/21/04 (H3N2) Increase Virus Growth in Embryonated Chicken Eggs." Vaccine, vol. 24, no. 44-46, 2006, pp. 6691-3.
Lu B, Zhou H, Chan W, et al. Single amino acid substitutions in the hemagglutinin of influenza A/Singapore/21/04 (H3N2) increase virus growth in embryonated chicken eggs. Vaccine. 2006;24(44-46):6691-3.
Lu, B., Zhou, H., Chan, W., Kemble, G., & Jin, H. (2006). Single amino acid substitutions in the hemagglutinin of influenza A/Singapore/21/04 (H3N2) increase virus growth in embryonated chicken eggs. Vaccine, 24(44-46), 6691-3.
Lu B, et al. Single Amino Acid Substitutions in the Hemagglutinin of Influenza A/Singapore/21/04 (H3N2) Increase Virus Growth in Embryonated Chicken Eggs. Vaccine. 2006 Nov 10;24(44-46):6691-3. PubMed PMID: 16814431.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Single amino acid substitutions in the hemagglutinin of influenza A/Singapore/21/04 (H3N2) increase virus growth in embryonated chicken eggs. AU - Lu,Bin, AU - Zhou,Helen, AU - Chan,Winnie, AU - Kemble,George, AU - Jin,Hong, Y1 - 2006/06/09/ PY - 2006/7/4/pubmed PY - 2007/1/24/medline PY - 2006/7/4/entrez SP - 6691 EP - 3 JF - Vaccine JO - Vaccine VL - 24 IS - 44-46 N2 - Most of the recently circulating H3N2 influenza A strains do not replicate well in embryonated chicken eggs and had to be isolated by cell culture, which presents a great challenge for influenza vaccine production using embryonated chicken eggs. We previously reported that a human H3N2 virus, A/Fujian/411/02, which replicates poorly in eggs, could be improved by changing a minimum of two HA residues (G186V/V226I or H183L/V226A). Here, we extended our work to the A/Singapore/21/04 strain that was also unable to grow in eggs. We showed that a single amino acid substitution of either G186V or A196T in the HA resulted in significantly increased virus replication in eggs without affecting virus antigenicity. SN - 0264-410X UR - https://www.unboundmedicine.com/medline/citation/16814431/Single_amino_acid_substitutions_in_the_hemagglutinin_of_influenza_A/Singapore/21/04__H3N2__increase_virus_growth_in_embryonated_chicken_eggs_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0264-410X(06)00645-1 DB - PRIME DP - Unbound Medicine ER -