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Neuroprotective effect of hyperbaric oxygen therapy monitored by MR-imaging after embolic stroke in rats.
Exp Neurol. 2006 Oct; 201(2):316-23.EN

Abstract

The potential neuroprotective effects of hyperbaric oxygen (HBO) were tested in an embolic model of focal cerebral ischemia with partially spontaneous reperfusion. Rats (n = 10) were subjected to embolic middle cerebral artery occlusion (MCAO) and diffusion weighted MRI (DWI) was performed at baseline, 1, 3, and 6 h after MCAO to determine the ADC viability threshold yielding the lesion volumes that best approximated the 2,3,5-triphenyltetrazolium chloride (TTC) infarct volumes at 24 h (experiment 1). For assessment of neuroprotective effects, rats were treated with 100% oxygen at 2.5 atmospheres absolute (ATA, n = 15) or normobaric room air (n = 15) for 60 min beginning 180 min after MCAO (experiment 2). DWI-, perfusion (PWI)- and T2-weighted MRI (T2WI) started within 0.5 h after MCAO and was continued 5 h, 24 h (PWI and T2WI only), and 168 h (T2WI only). Infarct volume was calculated based on TTC-staining at 24 h (experiment 1) or 168 h (experiment 2) post-MCAO. ADC-lesion evolution was maximal between 3 and 6 h. In experiment 2, the relative regional cerebral blood volume (rCBV) of both groups showed similar incomplete spontaneous reperfusion in the ischemic core. HBO reduced infarct volume to 145.3 +/- 39.6 mm3 vs. 202.5 +/- 58.3 mm3 (control, P = 0.029). As shown by MRI and TTC, HBO treatment demonstrated significant neuroprotection at 5 h after embolic focal cerebral ischemia that lasted for 168 h.

Authors+Show Affiliations

Department of Neurology, University of Massachusetts Medical School, Worcester, MA 01655, USA. Nils.Henninger@umassmed.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16814772

Citation

Henninger, Nils, et al. "Neuroprotective Effect of Hyperbaric Oxygen Therapy Monitored By MR-imaging After Embolic Stroke in Rats." Experimental Neurology, vol. 201, no. 2, 2006, pp. 316-23.
Henninger N, Küppers-Tiedt L, Sicard KM, et al. Neuroprotective effect of hyperbaric oxygen therapy monitored by MR-imaging after embolic stroke in rats. Exp Neurol. 2006;201(2):316-23.
Henninger, N., Küppers-Tiedt, L., Sicard, K. M., Günther, A., Schneider, D., & Schwab, S. (2006). Neuroprotective effect of hyperbaric oxygen therapy monitored by MR-imaging after embolic stroke in rats. Experimental Neurology, 201(2), 316-23.
Henninger N, et al. Neuroprotective Effect of Hyperbaric Oxygen Therapy Monitored By MR-imaging After Embolic Stroke in Rats. Exp Neurol. 2006;201(2):316-23. PubMed PMID: 16814772.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuroprotective effect of hyperbaric oxygen therapy monitored by MR-imaging after embolic stroke in rats. AU - Henninger,Nils, AU - Küppers-Tiedt,Lea, AU - Sicard,Kenneth M, AU - Günther,Albrecht, AU - Schneider,Dietmar, AU - Schwab,Stefan, Y1 - 2006/06/30/ PY - 2006/02/28/received PY - 2006/04/11/revised PY - 2006/04/18/accepted PY - 2006/7/4/pubmed PY - 2006/12/9/medline PY - 2006/7/4/entrez SP - 316 EP - 23 JF - Experimental neurology JO - Exp. Neurol. VL - 201 IS - 2 N2 - The potential neuroprotective effects of hyperbaric oxygen (HBO) were tested in an embolic model of focal cerebral ischemia with partially spontaneous reperfusion. Rats (n = 10) were subjected to embolic middle cerebral artery occlusion (MCAO) and diffusion weighted MRI (DWI) was performed at baseline, 1, 3, and 6 h after MCAO to determine the ADC viability threshold yielding the lesion volumes that best approximated the 2,3,5-triphenyltetrazolium chloride (TTC) infarct volumes at 24 h (experiment 1). For assessment of neuroprotective effects, rats were treated with 100% oxygen at 2.5 atmospheres absolute (ATA, n = 15) or normobaric room air (n = 15) for 60 min beginning 180 min after MCAO (experiment 2). DWI-, perfusion (PWI)- and T2-weighted MRI (T2WI) started within 0.5 h after MCAO and was continued 5 h, 24 h (PWI and T2WI only), and 168 h (T2WI only). Infarct volume was calculated based on TTC-staining at 24 h (experiment 1) or 168 h (experiment 2) post-MCAO. ADC-lesion evolution was maximal between 3 and 6 h. In experiment 2, the relative regional cerebral blood volume (rCBV) of both groups showed similar incomplete spontaneous reperfusion in the ischemic core. HBO reduced infarct volume to 145.3 +/- 39.6 mm3 vs. 202.5 +/- 58.3 mm3 (control, P = 0.029). As shown by MRI and TTC, HBO treatment demonstrated significant neuroprotection at 5 h after embolic focal cerebral ischemia that lasted for 168 h. SN - 0014-4886 UR - https://www.unboundmedicine.com/medline/citation/16814772/Neuroprotective_effect_of_hyperbaric_oxygen_therapy_monitored_by_MR_imaging_after_embolic_stroke_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-4886(06)00267-6 DB - PRIME DP - Unbound Medicine ER -