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Dietary supplementation with antioxidants improves functions and decreases oxidative stress of leukocytes from prematurely aging mice.
Nutrition. 2006 Jul-Aug; 22(7-8):767-77.N

Abstract

OBJECTIVES

Aging is accompanied by chronic inflammation and oxidative stress, which lead to a marked impairment of immune function and therefore increased mortality. This study assessed the effect of dietary supplementation, for 15 wk, with 5% and 20% (w/w) of biscuits enriched with nutritional doses of vitamins C and E, zinc, selenium, and beta-carotenes on function and oxidative stress parameters of peritoneal leukocytes from middle-aged, prematurely aging mice (PAM) and non-prematurely aging mice (NPAM).

METHODS

After supplementation we measured leukocyte functions (adherence, chemotaxis, phagocytosis, intracellular reactive oxygen species levels, lymphoproliferation, natural killer activity, and interleukin-2 release), antioxidant defenses (superoxide dismutase, glutathione peroxidase, and reduced glutathione), oxidant compounds (extracellular O(2)(-), glutathione disulfide, glutathione disulfide/reduced glutathione ratio, tumor necrosis factor-alpha, nitric oxide, and prostaglandin E(2)), and lipid and DNA oxidative damage, measured by malondialdehyde and 8-oxo,7,8-dihydro-2'-deoxyguanosine levels, respectively.

RESULTS

In general, leukocyte functions were improved and redox homeostasis was restored after intake of antioxidants. In consequence, malondialdehyde and 8-oxo,7,8-dihydro-2'-deoxyguanosine in PAM and NPAM were strikingly decreased after 5% and 20% supplementation (malondialdehyde, P < 0.001 in PAM; P < 0.01 in NPAM after both treatments; 8-oxo,7,8-dihydro-2'-deoxyguanosine, P < 0.01 after 5% supplementation and P < 0.001 after 20% supplementation in PAM and NPAM). Moreover, the effect of the antioxidants was stronger in PAM than in NPAM, and 20% supplementation was more effective than 5%.

CONCLUSION

Our data suggest that improvement of leukocyte function and restoration of redox balance after consumption of adequate levels of antioxidants from adulthood may be useful to attain healthy aging, especially in animals with premature aging.

Authors+Show Affiliations

Department of Physiology (Animal Physiology II), Faculty of Biological Sciences, Complutense University, Madrid, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16815491

Citation

Alvarado, Carmen, et al. "Dietary Supplementation With Antioxidants Improves Functions and Decreases Oxidative Stress of Leukocytes From Prematurely Aging Mice." Nutrition (Burbank, Los Angeles County, Calif.), vol. 22, no. 7-8, 2006, pp. 767-77.
Alvarado C, Alvarez P, Puerto M, et al. Dietary supplementation with antioxidants improves functions and decreases oxidative stress of leukocytes from prematurely aging mice. Nutrition. 2006;22(7-8):767-77.
Alvarado, C., Alvarez, P., Puerto, M., Gausserès, N., Jiménez, L., & De la Fuente, M. (2006). Dietary supplementation with antioxidants improves functions and decreases oxidative stress of leukocytes from prematurely aging mice. Nutrition (Burbank, Los Angeles County, Calif.), 22(7-8), 767-77.
Alvarado C, et al. Dietary Supplementation With Antioxidants Improves Functions and Decreases Oxidative Stress of Leukocytes From Prematurely Aging Mice. Nutrition. 2006 Jul-Aug;22(7-8):767-77. PubMed PMID: 16815491.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dietary supplementation with antioxidants improves functions and decreases oxidative stress of leukocytes from prematurely aging mice. AU - Alvarado,Carmen, AU - Alvarez,Pedro, AU - Puerto,Marta, AU - Gausserès,Nicolas, AU - Jiménez,Liliana, AU - De la Fuente,Mónica, PY - 2005/11/04/received PY - 2006/04/28/revised PY - 2006/05/10/accepted PY - 2006/7/4/pubmed PY - 2006/12/9/medline PY - 2006/7/4/entrez SP - 767 EP - 77 JF - Nutrition (Burbank, Los Angeles County, Calif.) JO - Nutrition VL - 22 IS - 7-8 N2 - OBJECTIVES: Aging is accompanied by chronic inflammation and oxidative stress, which lead to a marked impairment of immune function and therefore increased mortality. This study assessed the effect of dietary supplementation, for 15 wk, with 5% and 20% (w/w) of biscuits enriched with nutritional doses of vitamins C and E, zinc, selenium, and beta-carotenes on function and oxidative stress parameters of peritoneal leukocytes from middle-aged, prematurely aging mice (PAM) and non-prematurely aging mice (NPAM). METHODS: After supplementation we measured leukocyte functions (adherence, chemotaxis, phagocytosis, intracellular reactive oxygen species levels, lymphoproliferation, natural killer activity, and interleukin-2 release), antioxidant defenses (superoxide dismutase, glutathione peroxidase, and reduced glutathione), oxidant compounds (extracellular O(2)(-), glutathione disulfide, glutathione disulfide/reduced glutathione ratio, tumor necrosis factor-alpha, nitric oxide, and prostaglandin E(2)), and lipid and DNA oxidative damage, measured by malondialdehyde and 8-oxo,7,8-dihydro-2'-deoxyguanosine levels, respectively. RESULTS: In general, leukocyte functions were improved and redox homeostasis was restored after intake of antioxidants. In consequence, malondialdehyde and 8-oxo,7,8-dihydro-2'-deoxyguanosine in PAM and NPAM were strikingly decreased after 5% and 20% supplementation (malondialdehyde, P < 0.001 in PAM; P < 0.01 in NPAM after both treatments; 8-oxo,7,8-dihydro-2'-deoxyguanosine, P < 0.01 after 5% supplementation and P < 0.001 after 20% supplementation in PAM and NPAM). Moreover, the effect of the antioxidants was stronger in PAM than in NPAM, and 20% supplementation was more effective than 5%. CONCLUSION: Our data suggest that improvement of leukocyte function and restoration of redox balance after consumption of adequate levels of antioxidants from adulthood may be useful to attain healthy aging, especially in animals with premature aging. SN - 0899-9007 UR - https://www.unboundmedicine.com/medline/citation/16815491/Dietary_supplementation_with_antioxidants_improves_functions_and_decreases_oxidative_stress_of_leukocytes_from_prematurely_aging_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0899-9007(06)00225-5 DB - PRIME DP - Unbound Medicine ER -