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Capillary electrophoresis-mass spectrometry, an attractive tool for drug bioanalysis and biomarker discovery.
Electrophoresis. 2006 Jul; 27(13):2616-29.E

Abstract

The coupling of CE with MS detection, a relatively recent hyphenated technique, has gained increasing respect in the field of bioanalytical applications over the past few years. The first part of this review presents CE-MS applications dealing with drug bioanalysis, including forensic analysis and metabolism studies. Practical considerations to achieve a robust and sensitive CE-MS coupling are also presented. It is indeed essential to strictly control some critical electrospray parameters, such as the sheath liquid composition and flow rate, the nebulizing gas pressure as well as the capillary outlet position. The second part of the review critically describes the applications of CE coupled on-line to MS for the identification of biomarkers in body fluids for diagnostic purposes. Since the sample preparation procedures strongly differ according to the intended use (drug bioanalysis or biomarker discovery), they are discussed separately, taking into account the particular properties of plasma and urine matrices.

Authors+Show Affiliations

Servais AC
Department of Analytical Pharmaceutical Chemistry, Institute of Pharmacy, University of Liège, Belgium.
Crommen J
No affiliation info available
Fillet M
No affiliation info available

MeSH

AnimalsBiomarkersElectrophoresis, CapillaryHumansMass SpectrometryPharmaceutical PreparationsSensitivity and Specificity

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

16817163

Citation

Servais, Anne-Catherine, et al. "Capillary Electrophoresis-mass Spectrometry, an Attractive Tool for Drug Bioanalysis and Biomarker Discovery." Electrophoresis, vol. 27, no. 13, 2006, pp. 2616-29.
Servais AC, Crommen J, Fillet M. Capillary electrophoresis-mass spectrometry, an attractive tool for drug bioanalysis and biomarker discovery. Electrophoresis. 2006;27(13):2616-29.
Servais, A. C., Crommen, J., & Fillet, M. (2006). Capillary electrophoresis-mass spectrometry, an attractive tool for drug bioanalysis and biomarker discovery. Electrophoresis, 27(13), 2616-29.
Servais AC, Crommen J, Fillet M. Capillary Electrophoresis-mass Spectrometry, an Attractive Tool for Drug Bioanalysis and Biomarker Discovery. Electrophoresis. 2006;27(13):2616-29. PubMed PMID: 16817163.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Capillary electrophoresis-mass spectrometry, an attractive tool for drug bioanalysis and biomarker discovery. AU - Servais,Anne-Catherine, AU - Crommen,Jacques, AU - Fillet,Marianne, PY - 2006/7/4/pubmed PY - 2006/9/16/medline PY - 2006/7/4/entrez SP - 2616 EP - 29 JF - Electrophoresis JO - Electrophoresis VL - 27 IS - 13 N2 - The coupling of CE with MS detection, a relatively recent hyphenated technique, has gained increasing respect in the field of bioanalytical applications over the past few years. The first part of this review presents CE-MS applications dealing with drug bioanalysis, including forensic analysis and metabolism studies. Practical considerations to achieve a robust and sensitive CE-MS coupling are also presented. It is indeed essential to strictly control some critical electrospray parameters, such as the sheath liquid composition and flow rate, the nebulizing gas pressure as well as the capillary outlet position. The second part of the review critically describes the applications of CE coupled on-line to MS for the identification of biomarkers in body fluids for diagnostic purposes. Since the sample preparation procedures strongly differ according to the intended use (drug bioanalysis or biomarker discovery), they are discussed separately, taking into account the particular properties of plasma and urine matrices. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/16817163/Capillary_electrophoresis_mass_spectrometry_an_attractive_tool_for_drug_bioanalysis_and_biomarker_discovery_ L2 - https://doi.org/10.1002/elps.200500934 DB - PRIME DP - Unbound Medicine ER -