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Lipid oxidation may reduce the quality of a fresh-frozen bone allograft. Is the approved storage temperature too high?
Acta Orthop 2006; 77(3):418-21AO

Abstract

BACKGROUND

International standards indicate that bone allografts for human use should be stored in a -40 degrees C or colder environment and the storage time should be up to 5 years. Bone allografts contain lipids which oxidate and become toxic with time.

MATERIAL AND METHODS

We determined lipid oxidation in femoral head bone allografts by headspace gas chromatography at different storage temperatures and storage times.

RESULTS

We found that lipid oxidation in fresh-frozen bone allograft was influenced by storage temperature and storage time. Lipid oxidation was significantly more rapid at -30 degrees C than at -70 degrees C. Even at -70 degrees C, however, some oxidation occurred but it was quite minimal after 3 years.

INTERPRETATION

Because of the negative effects of lipid oxidation, we recommend a storage temperature of -70 degrees C or lower for bone allografts.

Authors+Show Affiliations

Department of Orthopedics and Traumatology, University Hospital of Tampere and Medical School, University of Tampere. Tampere, FI-33521. Finland. minna.laitinen@pshp.fiNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

16819680

Citation

Laitinen, Minna, et al. "Lipid Oxidation May Reduce the Quality of a Fresh-frozen Bone Allograft. Is the Approved Storage Temperature Too High?" Acta Orthopaedica, vol. 77, no. 3, 2006, pp. 418-21.
Laitinen M, Kivikari R, Hirn M. Lipid oxidation may reduce the quality of a fresh-frozen bone allograft. Is the approved storage temperature too high? Acta Orthop. 2006;77(3):418-21.
Laitinen, M., Kivikari, R., & Hirn, M. (2006). Lipid oxidation may reduce the quality of a fresh-frozen bone allograft. Is the approved storage temperature too high? Acta Orthopaedica, 77(3), pp. 418-21.
Laitinen M, Kivikari R, Hirn M. Lipid Oxidation May Reduce the Quality of a Fresh-frozen Bone Allograft. Is the Approved Storage Temperature Too High. Acta Orthop. 2006;77(3):418-21. PubMed PMID: 16819680.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lipid oxidation may reduce the quality of a fresh-frozen bone allograft. Is the approved storage temperature too high? AU - Laitinen,Minna, AU - Kivikari,Riitta, AU - Hirn,Martti, PY - 2006/7/5/pubmed PY - 2006/8/5/medline PY - 2006/7/5/entrez SP - 418 EP - 21 JF - Acta orthopaedica JO - Acta Orthop VL - 77 IS - 3 N2 - BACKGROUND: International standards indicate that bone allografts for human use should be stored in a -40 degrees C or colder environment and the storage time should be up to 5 years. Bone allografts contain lipids which oxidate and become toxic with time. MATERIAL AND METHODS: We determined lipid oxidation in femoral head bone allografts by headspace gas chromatography at different storage temperatures and storage times. RESULTS: We found that lipid oxidation in fresh-frozen bone allograft was influenced by storage temperature and storage time. Lipid oxidation was significantly more rapid at -30 degrees C than at -70 degrees C. Even at -70 degrees C, however, some oxidation occurred but it was quite minimal after 3 years. INTERPRETATION: Because of the negative effects of lipid oxidation, we recommend a storage temperature of -70 degrees C or lower for bone allografts. SN - 1745-3674 UR - https://www.unboundmedicine.com/medline/citation/16819680/Lipid_oxidation_may_reduce_the_quality_of_a_fresh_frozen_bone_allograft__Is_the_approved_storage_temperature_too_high L2 - http://www.tandfonline.com/doi/full/10.1080/17453670610046343 DB - PRIME DP - Unbound Medicine ER -