Tags

Type your tag names separated by a space and hit enter

Determination of agmatine in biological samples by capillary electrophoresis with optical fiber light-emitting-diode-induced fluorescence detection.
J Chromatogr A. 2006 Aug 04; 1123(1):138-41.JC

Abstract

A capillary electrophoresis (CE)/optical fiber light-emitting diode (LED)-induced fluorescence detection method is developed for the determination of agmatine in biological samples. The agmatine was precolumn-derivatized with fluorescence tagging reagent, fluorescein isothiocyanate (FITC). Optimal separation and determination for agmatine were obtained with an electrophoretic buffer of 20 mM sodium borate (pH 9.2). Under the optimal conditions, the determination of agmatine was achieved in less than 4 min, and the detection limit was 4.1x10(-9) M (S/N = 3). The relative standard deviation (RSD) for 11 parallel determination of agmatine was less than 3.0%. The present CE-LED induced fluorescence detection method has been applied to detect agmatine in rat brain tissue, rat stomach tissue, human serum, and human urine. The level of agmatine in human urine was quantified by CE for the first time and found to be in the range 2.5-4.1x10(-7) M.

Authors+Show Affiliations

College of Chemical Engineering, Sichuan University, Chengdu 610065, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16820162

Citation

Zhao, Shulin, et al. "Determination of Agmatine in Biological Samples By Capillary Electrophoresis With Optical Fiber Light-emitting-diode-induced Fluorescence Detection." Journal of Chromatography. A, vol. 1123, no. 1, 2006, pp. 138-41.
Zhao S, Wang B, Yuan H, et al. Determination of agmatine in biological samples by capillary electrophoresis with optical fiber light-emitting-diode-induced fluorescence detection. J Chromatogr A. 2006;1123(1):138-41.
Zhao, S., Wang, B., Yuan, H., & Xiao, D. (2006). Determination of agmatine in biological samples by capillary electrophoresis with optical fiber light-emitting-diode-induced fluorescence detection. Journal of Chromatography. A, 1123(1), 138-41.
Zhao S, et al. Determination of Agmatine in Biological Samples By Capillary Electrophoresis With Optical Fiber Light-emitting-diode-induced Fluorescence Detection. J Chromatogr A. 2006 Aug 4;1123(1):138-41. PubMed PMID: 16820162.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of agmatine in biological samples by capillary electrophoresis with optical fiber light-emitting-diode-induced fluorescence detection. AU - Zhao,Shulin, AU - Wang,Bing, AU - Yuan,Hongyan, AU - Xiao,Dan, Y1 - 2006/07/03/ PY - 2006/01/10/received PY - 2006/05/06/revised PY - 2006/05/15/accepted PY - 2006/7/6/pubmed PY - 2006/9/26/medline PY - 2006/7/6/entrez SP - 138 EP - 41 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1123 IS - 1 N2 - A capillary electrophoresis (CE)/optical fiber light-emitting diode (LED)-induced fluorescence detection method is developed for the determination of agmatine in biological samples. The agmatine was precolumn-derivatized with fluorescence tagging reagent, fluorescein isothiocyanate (FITC). Optimal separation and determination for agmatine were obtained with an electrophoretic buffer of 20 mM sodium borate (pH 9.2). Under the optimal conditions, the determination of agmatine was achieved in less than 4 min, and the detection limit was 4.1x10(-9) M (S/N = 3). The relative standard deviation (RSD) for 11 parallel determination of agmatine was less than 3.0%. The present CE-LED induced fluorescence detection method has been applied to detect agmatine in rat brain tissue, rat stomach tissue, human serum, and human urine. The level of agmatine in human urine was quantified by CE for the first time and found to be in the range 2.5-4.1x10(-7) M. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/16820162/Determination_of_agmatine_in_biological_samples_by_capillary_electrophoresis_with_optical_fiber_light_emitting_diode_induced_fluorescence_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(06)01017-X DB - PRIME DP - Unbound Medicine ER -