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Simultaneous determination of bromvalerylurea, bromodiethylacetylurea, and allylisopropylacetylurea in serum and urine by high-performance liquid chromatography with a multiwavelength UV detector and thin-layer chromatography.
J Anal Toxicol. 1991 May-Jun; 15(3):123-5.JA

Abstract

A method for rapid detection and identification of bromvalerylurea (BVU), bromodiethylacetylurea (BDU), and allylisopropylacetylurea (AIU) in serum and urine by high-performance liquid chromatography (HPLC) with a multiwavelength UV detector after Sep-Pak C18 cartridge extraction is reported. A Jasco Finepak C18 reversed-phase column was used for the separation. Acetonitrile-distilled water (1:1, v/v) was used as a mobile phase. There was no significant absorption of the three hypnotics in the UV spectra (210-350 nm). However, the absorption of each was higher at the shorter wavelengths. The quantifications for the three hypnotics detected at 210 nm by the chromatogram were linear over the range 0.2-4 micrograms/mL and the detection limits of BVU, BDU, and AIU were 5, 10, and 10 ng as absolute amounts, respectively. The mean recovery yields of BVU, BDU, and AIU by Sep-Pak C18 cartridge extraction were 85.7 +/- 4.1, 98.6 +/- 2.2, and 95.1 +/- 3.5% (n = 5) in serum and 79.5 +/- 3.8, 95.7 +/- 1.8, and 93.0 +/- 4.2% (n = 5) in urine, respectively. An optimal system of thin-layer chromatography for the identification of the hypnotics is also discussed.

Authors+Show Affiliations

Identification Section, Kagawa Pref. Police H.Q., Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1682528

Citation

Miyauchi, H, et al. "Simultaneous Determination of Bromvalerylurea, Bromodiethylacetylurea, and Allylisopropylacetylurea in Serum and Urine By High-performance Liquid Chromatography With a Multiwavelength UV Detector and Thin-layer Chromatography." Journal of Analytical Toxicology, vol. 15, no. 3, 1991, pp. 123-5.
Miyauchi H, Ameno K, Fuke C, et al. Simultaneous determination of bromvalerylurea, bromodiethylacetylurea, and allylisopropylacetylurea in serum and urine by high-performance liquid chromatography with a multiwavelength UV detector and thin-layer chromatography. J Anal Toxicol. 1991;15(3):123-5.
Miyauchi, H., Ameno, K., Fuke, C., Ameno, S., & Ijiri, I. (1991). Simultaneous determination of bromvalerylurea, bromodiethylacetylurea, and allylisopropylacetylurea in serum and urine by high-performance liquid chromatography with a multiwavelength UV detector and thin-layer chromatography. Journal of Analytical Toxicology, 15(3), 123-5.
Miyauchi H, et al. Simultaneous Determination of Bromvalerylurea, Bromodiethylacetylurea, and Allylisopropylacetylurea in Serum and Urine By High-performance Liquid Chromatography With a Multiwavelength UV Detector and Thin-layer Chromatography. J Anal Toxicol. 1991 May-Jun;15(3):123-5. PubMed PMID: 1682528.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of bromvalerylurea, bromodiethylacetylurea, and allylisopropylacetylurea in serum and urine by high-performance liquid chromatography with a multiwavelength UV detector and thin-layer chromatography. AU - Miyauchi,H, AU - Ameno,K, AU - Fuke,C, AU - Ameno,S, AU - Ijiri,I, PY - 1991/5/1/pubmed PY - 1991/5/1/medline PY - 1991/5/1/entrez SP - 123 EP - 5 JF - Journal of analytical toxicology JO - J Anal Toxicol VL - 15 IS - 3 N2 - A method for rapid detection and identification of bromvalerylurea (BVU), bromodiethylacetylurea (BDU), and allylisopropylacetylurea (AIU) in serum and urine by high-performance liquid chromatography (HPLC) with a multiwavelength UV detector after Sep-Pak C18 cartridge extraction is reported. A Jasco Finepak C18 reversed-phase column was used for the separation. Acetonitrile-distilled water (1:1, v/v) was used as a mobile phase. There was no significant absorption of the three hypnotics in the UV spectra (210-350 nm). However, the absorption of each was higher at the shorter wavelengths. The quantifications for the three hypnotics detected at 210 nm by the chromatogram were linear over the range 0.2-4 micrograms/mL and the detection limits of BVU, BDU, and AIU were 5, 10, and 10 ng as absolute amounts, respectively. The mean recovery yields of BVU, BDU, and AIU by Sep-Pak C18 cartridge extraction were 85.7 +/- 4.1, 98.6 +/- 2.2, and 95.1 +/- 3.5% (n = 5) in serum and 79.5 +/- 3.8, 95.7 +/- 1.8, and 93.0 +/- 4.2% (n = 5) in urine, respectively. An optimal system of thin-layer chromatography for the identification of the hypnotics is also discussed. SN - 0146-4760 UR - https://www.unboundmedicine.com/medline/citation/1682528/Simultaneous_determination_of_bromvalerylurea_bromodiethylacetylurea_and_allylisopropylacetylurea_in_serum_and_urine_by_high_performance_liquid_chromatography_with_a_multiwavelength_UV_detector_and_thin_layer_chromatography_ L2 - https://academic.oup.com/jat/article-lookup/doi/10.1093/jat/15.3.123 DB - PRIME DP - Unbound Medicine ER -