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Nonrandom distribution of Burkholderia pseudomallei clones in relation to geographical location and virulence.
J Clin Microbiol. 2006 Jul; 44(7):2553-7.JC

Abstract

Burkholderia pseudomallei is a soil-dwelling saprophyte and the causative agent of melioidosis, a life-threatening human infection. Most cases are reported from northeast Thailand and northern Australia. Using multilocus sequence typing (MLST), we have compared (i) soil and invasive isolates from northeast Thailand and (ii) invasive isolates from Thailand and Australia. A total of 266 Thai B. pseudomallei isolates were characterized (83 soil and 183 invasive). These corresponded to 123 sequence types (STs), the most abundant being ST70 (n=21), ST167 (n=15), ST54 (n=12), and ST58 (n=11). Two clusters of related STs (clonal complexes) were identified; the larger clonal complex (CC48) did not conform to a simple pattern of radial expansion from an assumed ancestor, while a second (CC70) corresponded to a simple radial expansion from ST70. Despite the large number of STs, overall nucleotide diversity was low. Of the Thai isolates, those isolated from patients with melioidosis were overrepresented in the 10 largest clones (P<0.0001). There was a significant difference in the classification index between environmental and disease isolates (P<0.001), confirming that genotypes were not distributed randomly between the two samples. MLST profiles for 158 isolates from Australia (mainly disease associated) contained a number of STs (96) similar to that seen with the Thai invasive isolates, but no ST was found in both populations. There were also differences in diversity and allele frequency distribution between the two populations. This analysis reveals strong genetic differentiation on the basis of geographical isolation and a significant differentiation on the basis of virulence potential.

Authors+Show Affiliations

Faculty of Tropical Medicine, Mahidol University, 420/6 Rajvithi Road, Bangkok 10400, Thailand, and Churchill Hospital, Oxford, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16825379

Citation

Vesaratchavest, Mongkol, et al. "Nonrandom Distribution of Burkholderia Pseudomallei Clones in Relation to Geographical Location and Virulence." Journal of Clinical Microbiology, vol. 44, no. 7, 2006, pp. 2553-7.
Vesaratchavest M, Tumapa S, Day NP, et al. Nonrandom distribution of Burkholderia pseudomallei clones in relation to geographical location and virulence. J Clin Microbiol. 2006;44(7):2553-7.
Vesaratchavest, M., Tumapa, S., Day, N. P., Wuthiekanun, V., Chierakul, W., Holden, M. T., White, N. J., Currie, B. J., Spratt, B. G., Feil, E. J., & Peacock, S. J. (2006). Nonrandom distribution of Burkholderia pseudomallei clones in relation to geographical location and virulence. Journal of Clinical Microbiology, 44(7), 2553-7.
Vesaratchavest M, et al. Nonrandom Distribution of Burkholderia Pseudomallei Clones in Relation to Geographical Location and Virulence. J Clin Microbiol. 2006;44(7):2553-7. PubMed PMID: 16825379.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nonrandom distribution of Burkholderia pseudomallei clones in relation to geographical location and virulence. AU - Vesaratchavest,Mongkol, AU - Tumapa,Sarinna, AU - Day,Nicholas P J, AU - Wuthiekanun,Vanaporn, AU - Chierakul,Wirongrong, AU - Holden,Matthew T G, AU - White,Nicholas J, AU - Currie,Bart J, AU - Spratt,Brian G, AU - Feil,Edward J, AU - Peacock,Sharon J, PY - 2006/7/11/pubmed PY - 2006/10/25/medline PY - 2006/7/11/entrez SP - 2553 EP - 7 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 44 IS - 7 N2 - Burkholderia pseudomallei is a soil-dwelling saprophyte and the causative agent of melioidosis, a life-threatening human infection. Most cases are reported from northeast Thailand and northern Australia. Using multilocus sequence typing (MLST), we have compared (i) soil and invasive isolates from northeast Thailand and (ii) invasive isolates from Thailand and Australia. A total of 266 Thai B. pseudomallei isolates were characterized (83 soil and 183 invasive). These corresponded to 123 sequence types (STs), the most abundant being ST70 (n=21), ST167 (n=15), ST54 (n=12), and ST58 (n=11). Two clusters of related STs (clonal complexes) were identified; the larger clonal complex (CC48) did not conform to a simple pattern of radial expansion from an assumed ancestor, while a second (CC70) corresponded to a simple radial expansion from ST70. Despite the large number of STs, overall nucleotide diversity was low. Of the Thai isolates, those isolated from patients with melioidosis were overrepresented in the 10 largest clones (P<0.0001). There was a significant difference in the classification index between environmental and disease isolates (P<0.001), confirming that genotypes were not distributed randomly between the two samples. MLST profiles for 158 isolates from Australia (mainly disease associated) contained a number of STs (96) similar to that seen with the Thai invasive isolates, but no ST was found in both populations. There were also differences in diversity and allele frequency distribution between the two populations. This analysis reveals strong genetic differentiation on the basis of geographical isolation and a significant differentiation on the basis of virulence potential. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/16825379/Nonrandom_distribution_of_Burkholderia_pseudomallei_clones_in_relation_to_geographical_location_and_virulence_ L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&amp;pmid=16825379 DB - PRIME DP - Unbound Medicine ER -