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Iron absorption by healthy women is not associated with either serum or urinary prohepcidin.
Am J Clin Nutr. 2006 Jul; 84(1):150-5.AJ

Abstract

BACKGROUND

Although hepcidin is proposed as a regulator of iron absorption, this has not been assessed in humans.

OBJECTIVE

Our objective was to assess the relation between serum or urinary prohepcidin and iron absorption in healthy premenopausal women.

DESIGN

The subjects were 28 healthy women aged 22-51 y with normal hemoglobin concentrations (120-152 g/L). Absorption of 0.5 mg Fe with 0.2 microCi 59Fe tracer, both as FeSO4, was measured by whole-body scintillation counting 13 d after oral administration. Fasting blood and urine samples were collected the day of and 16 wk after the absorption measurement. Serum and urinary prohepcidin concentrations were measured by an enzyme-linked immunosorbent assay by using an antibody against amino acid residues 28-47 of the proregion.

RESULTS

Mean (+/-SD) iron absorption was 36 +/- 19% (range: 4-81%), and serum ferritin (geometric x) was 27 microg/L (range: 4-122 microg/L), as commonly observed in healthy premenopausal women. Serum prohepcidin was 196 microg/L (range: 99-376 microg/L) and, in contrast with urinary prohepcidin, was relatively consistent for the women between 0 and 16 wk. Serum prohepcidin correlated directly with serum ferritin (R2 = 0.28, P < 0.01) but was unrelated to 59Fe absorption, in contrast to serum ferritin (R2 = 0.33, P < 0.01).

CONCLUSIONS

Serum prohepcidin concentrations were relatively stable within subjects and correlated with serum ferritin. However, unlike serum ferritin, neither serum nor urinary prohepcidin concentrations were related to iron absorption in healthy women.

Authors+Show Affiliations

US Department of Agriculture, Agricultural Research Service, Grand Forks Human Nutrition Research Center, Grand Forks, ND 58202-9034, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

16825689

Citation

Hadley, Kevin B., et al. "Iron Absorption By Healthy Women Is Not Associated With Either Serum or Urinary Prohepcidin." The American Journal of Clinical Nutrition, vol. 84, no. 1, 2006, pp. 150-5.
Hadley KB, Johnson LK, Hunt JR. Iron absorption by healthy women is not associated with either serum or urinary prohepcidin. Am J Clin Nutr. 2006;84(1):150-5.
Hadley, K. B., Johnson, L. K., & Hunt, J. R. (2006). Iron absorption by healthy women is not associated with either serum or urinary prohepcidin. The American Journal of Clinical Nutrition, 84(1), 150-5.
Hadley KB, Johnson LK, Hunt JR. Iron Absorption By Healthy Women Is Not Associated With Either Serum or Urinary Prohepcidin. Am J Clin Nutr. 2006;84(1):150-5. PubMed PMID: 16825689.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Iron absorption by healthy women is not associated with either serum or urinary prohepcidin. AU - Hadley,Kevin B, AU - Johnson,LuAnn K, AU - Hunt,Janet R, PY - 2006/7/11/pubmed PY - 2006/8/23/medline PY - 2006/7/11/entrez SP - 150 EP - 5 JF - The American journal of clinical nutrition JO - Am. J. Clin. Nutr. VL - 84 IS - 1 N2 - BACKGROUND: Although hepcidin is proposed as a regulator of iron absorption, this has not been assessed in humans. OBJECTIVE: Our objective was to assess the relation between serum or urinary prohepcidin and iron absorption in healthy premenopausal women. DESIGN: The subjects were 28 healthy women aged 22-51 y with normal hemoglobin concentrations (120-152 g/L). Absorption of 0.5 mg Fe with 0.2 microCi 59Fe tracer, both as FeSO4, was measured by whole-body scintillation counting 13 d after oral administration. Fasting blood and urine samples were collected the day of and 16 wk after the absorption measurement. Serum and urinary prohepcidin concentrations were measured by an enzyme-linked immunosorbent assay by using an antibody against amino acid residues 28-47 of the proregion. RESULTS: Mean (+/-SD) iron absorption was 36 +/- 19% (range: 4-81%), and serum ferritin (geometric x) was 27 microg/L (range: 4-122 microg/L), as commonly observed in healthy premenopausal women. Serum prohepcidin was 196 microg/L (range: 99-376 microg/L) and, in contrast with urinary prohepcidin, was relatively consistent for the women between 0 and 16 wk. Serum prohepcidin correlated directly with serum ferritin (R2 = 0.28, P < 0.01) but was unrelated to 59Fe absorption, in contrast to serum ferritin (R2 = 0.33, P < 0.01). CONCLUSIONS: Serum prohepcidin concentrations were relatively stable within subjects and correlated with serum ferritin. However, unlike serum ferritin, neither serum nor urinary prohepcidin concentrations were related to iron absorption in healthy women. SN - 0002-9165 UR - https://www.unboundmedicine.com/medline/citation/16825689/Iron_absorption_by_healthy_women_is_not_associated_with_either_serum_or_urinary_prohepcidin_ L2 - https://academic.oup.com/ajcn/article-lookup/doi/10.1093/ajcn/84.1.150 DB - PRIME DP - Unbound Medicine ER -