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Determination of pesticides in wine using micellar electrokinetic chromatography with UV detection and sample stacking.
J Chromatogr A. 2007 May 25; 1150(1-2):348-55.JC

Abstract

In this work, the analysis of a group of four fungicides (pyrimethanil, nuarimol, procymidone and cyprodinil) and one insecticide (pirimicarb) by micellar electrokinetic chromatography (MEKC) with UV detection using the on-line preconcentration strategy called reversed electrode polarity stacking mode (REPSM) is proposed. After optimisation, an adequate separation electrolyte for the separation and stacking of these pesticides was obtained which consisted of 100 mM borate, 60 mM sodium dodecyl sulphate (SDS), at pH 9.0 and 2% 2-propanol. The use of this running buffer together with the REPSM preconcentration method provided limits of detection (LODs) between 38.3 and 241 microg/L. In order to apply the developed methodology for the analysis of these pesticides in wine samples, several off-line preconcentration strategies (mainly, solid-phase extraction, SPE, and solid-phase microextraction, SPME) were tested. Although the use of a SPE procedure, optimized in this work for water samples, using Oasis HLB cartridges, provided mean recovery values between 79 and 100% for spiked water samples, it could not be applied to the extraction of these pesticides from wine samples due to high interference from the sample matrix. However, the use of a SPME procedure using polydimethylsiloxane/divynilbenzene (PDMS/DVB) fibers allowed the selective extraction of four of the five pesticides which could be perfectly determined. The final combination of the off-line SPME and on-line REPSM preconcentration strategies allowed obtaining LODs between 17.6 and 32.3 microg/L.

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Authors+Show Affiliations

Molina-Mayo C
Department of Analytical Chemistry, Nutrition and Food Science, University of La Laguna, Avda. Astrofísico Fco. Sánchez s/n, 38071 La Laguna, Tenerife, Canary Islands, Spain.
Hernández-Borges J
No affiliation info available
Borges-Miquel TM
No affiliation info available
Rodríguez-Delgado MA
No affiliation info available

MeSH

Chromatography, Micellar Electrokinetic CapillaryPesticidesReproducibility of ResultsSolid Phase MicroextractionSpectrophotometry, UltravioletWine

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16828783

Citation

Molina-Mayo, Carlos, et al. "Determination of Pesticides in Wine Using Micellar Electrokinetic Chromatography With UV Detection and Sample Stacking." Journal of Chromatography. A, vol. 1150, no. 1-2, 2007, pp. 348-55.
Molina-Mayo C, Hernández-Borges J, Borges-Miquel TM, et al. Determination of pesticides in wine using micellar electrokinetic chromatography with UV detection and sample stacking. J Chromatogr A. 2007;1150(1-2):348-55.
Molina-Mayo, C., Hernández-Borges, J., Borges-Miquel, T. M., & Rodríguez-Delgado, M. A. (2007). Determination of pesticides in wine using micellar electrokinetic chromatography with UV detection and sample stacking. Journal of Chromatography. A, 1150(1-2), 348-55.
Molina-Mayo C, et al. Determination of Pesticides in Wine Using Micellar Electrokinetic Chromatography With UV Detection and Sample Stacking. J Chromatogr A. 2007 May 25;1150(1-2):348-55. PubMed PMID: 16828783.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of pesticides in wine using micellar electrokinetic chromatography with UV detection and sample stacking. AU - Molina-Mayo,Carlos, AU - Hernández-Borges,Javier, AU - Borges-Miquel,Teresa M, AU - Rodríguez-Delgado,Miguel Angel, Y1 - 2006/07/07/ PY - 2006/05/23/received PY - 2006/06/16/revised PY - 2006/06/21/accepted PY - 2006/7/11/pubmed PY - 2007/7/6/medline PY - 2006/7/11/entrez SP - 348 EP - 55 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1150 IS - 1-2 N2 - In this work, the analysis of a group of four fungicides (pyrimethanil, nuarimol, procymidone and cyprodinil) and one insecticide (pirimicarb) by micellar electrokinetic chromatography (MEKC) with UV detection using the on-line preconcentration strategy called reversed electrode polarity stacking mode (REPSM) is proposed. After optimisation, an adequate separation electrolyte for the separation and stacking of these pesticides was obtained which consisted of 100 mM borate, 60 mM sodium dodecyl sulphate (SDS), at pH 9.0 and 2% 2-propanol. The use of this running buffer together with the REPSM preconcentration method provided limits of detection (LODs) between 38.3 and 241 microg/L. In order to apply the developed methodology for the analysis of these pesticides in wine samples, several off-line preconcentration strategies (mainly, solid-phase extraction, SPE, and solid-phase microextraction, SPME) were tested. Although the use of a SPE procedure, optimized in this work for water samples, using Oasis HLB cartridges, provided mean recovery values between 79 and 100% for spiked water samples, it could not be applied to the extraction of these pesticides from wine samples due to high interference from the sample matrix. However, the use of a SPME procedure using polydimethylsiloxane/divynilbenzene (PDMS/DVB) fibers allowed the selective extraction of four of the five pesticides which could be perfectly determined. The final combination of the off-line SPME and on-line REPSM preconcentration strategies allowed obtaining LODs between 17.6 and 32.3 microg/L. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/16828783/Determination_of_pesticides_in_wine_using_micellar_electrokinetic_chromatography_with_UV_detection_and_sample_stacking_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(06)01251-9 DB - PRIME DP - Unbound Medicine ER -