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Role of inducible nitric oxide synthase expressed by alveolar macrophages in high mobility group box 1--induced acute lung injury.
Inflamm Res. 2006 May; 55(5):207-15.IR

Abstract

OBJECTIVE AND DESIGN

The role of inducible nitric oxide synthase (iNOS) expressed by alveolar macrophages in acute lung injury induced by high mobility group box 1 (HMGB1) was explored.

TREATMENTS

Primary rat alveolar macrophages (PRAMs) were stimulated with HMGB1 to analyze iNOS expression. Alveolar macrophages and iNOS were inhibited by gadolinium chloride and 1400W in rats challenged by HMGB1 intratracheally.

METHODS

Western Blot was applied to assay iNOS expression in PRAMs. Indices for acute lung injury in rats were measured. Immunocytochemistry was used to localize iNOS in squarebronchoalveolar lavage (BAL) cells. The enzyme activities of iNOS and constitutive nitric oxide synthase (cNOS) for BAL cells were determined.

RESULTS

A time- and concentration-dependent response of iNOS expression in PRAMs to HMGB1 induction was observed. Intratracheal instillation of HMGB1 produced persistently exacerbated acute lung inflammation, induction of iNOS in alveolar macrophages and increased lung nitric oxide production in rats. Abrogation of iNOS or macrophages attenuated lung inflammation, nitric oxide in BAL fluid, and iNOS activity of BAL cells, but had no significant effect on cNOS activity of BAL cells in rats challenged by HMGB1.

CONCLUSIONS

Inducible nitric oxide synthase expressed by alveolar macrophages facilitates the development of HMGB1-induced acute lung injury.

Authors+Show Affiliations

Department of Pathophysiology, Institute of Basic Medical Sciences, Peking Union Medical College & Chinese Academy of Medical Sciences, 5 Dong Dan San Tiao, Beijing, 100005, People's Republic of China. dbren-7194@163.comNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16830108

Citation

Ren, D, et al. "Role of Inducible Nitric Oxide Synthase Expressed By Alveolar Macrophages in High Mobility Group Box 1--induced Acute Lung Injury." Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.], vol. 55, no. 5, 2006, pp. 207-15.
Ren D, Sun R, Wang S. Role of inducible nitric oxide synthase expressed by alveolar macrophages in high mobility group box 1--induced acute lung injury. Inflamm Res. 2006;55(5):207-15.
Ren, D., Sun, R., & Wang, S. (2006). Role of inducible nitric oxide synthase expressed by alveolar macrophages in high mobility group box 1--induced acute lung injury. Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.], 55(5), 207-15.
Ren D, Sun R, Wang S. Role of Inducible Nitric Oxide Synthase Expressed By Alveolar Macrophages in High Mobility Group Box 1--induced Acute Lung Injury. Inflamm Res. 2006;55(5):207-15. PubMed PMID: 16830108.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of inducible nitric oxide synthase expressed by alveolar macrophages in high mobility group box 1--induced acute lung injury. AU - Ren,D, AU - Sun,R, AU - Wang,S, PY - 2006/7/11/pubmed PY - 2006/10/27/medline PY - 2006/7/11/entrez SP - 207 EP - 15 JF - Inflammation research : official journal of the European Histamine Research Society ... [et al.] JO - Inflamm Res VL - 55 IS - 5 N2 - OBJECTIVE AND DESIGN: The role of inducible nitric oxide synthase (iNOS) expressed by alveolar macrophages in acute lung injury induced by high mobility group box 1 (HMGB1) was explored. TREATMENTS: Primary rat alveolar macrophages (PRAMs) were stimulated with HMGB1 to analyze iNOS expression. Alveolar macrophages and iNOS were inhibited by gadolinium chloride and 1400W in rats challenged by HMGB1 intratracheally. METHODS: Western Blot was applied to assay iNOS expression in PRAMs. Indices for acute lung injury in rats were measured. Immunocytochemistry was used to localize iNOS in squarebronchoalveolar lavage (BAL) cells. The enzyme activities of iNOS and constitutive nitric oxide synthase (cNOS) for BAL cells were determined. RESULTS: A time- and concentration-dependent response of iNOS expression in PRAMs to HMGB1 induction was observed. Intratracheal instillation of HMGB1 produced persistently exacerbated acute lung inflammation, induction of iNOS in alveolar macrophages and increased lung nitric oxide production in rats. Abrogation of iNOS or macrophages attenuated lung inflammation, nitric oxide in BAL fluid, and iNOS activity of BAL cells, but had no significant effect on cNOS activity of BAL cells in rats challenged by HMGB1. CONCLUSIONS: Inducible nitric oxide synthase expressed by alveolar macrophages facilitates the development of HMGB1-induced acute lung injury. SN - 1023-3830 UR - https://www.unboundmedicine.com/medline/citation/16830108/Role_of_inducible_nitric_oxide_synthase_expressed_by_alveolar_macrophages_in_high_mobility_group_box_1__induced_acute_lung_injury_ L2 - https://dx.doi.org/10.1007/s00011-006-0072-2 DB - PRIME DP - Unbound Medicine ER -