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In vitro anti-cariogenic activity of dichloromethane fraction from Rheum undulatum L. root.
Arch Pharm Res 2006; 29(6):490-6AP

Abstract

This study aimed to evaluate in vitro effects of Rheum undulatum L. root on the development of dental caries, especially its effects on viability, dental plaque formation, and glycolytic acid production of Streptococcus mutans and Streptococcus sobrinus. Methanol extract of Rheum undulatum L. root and its fractions were prepared and tested. Among the test extract and fractions, dichloromethane fraction (DF) showed the most active antibacterial activity (inhibition zone: 13-17 mm) against S. mutans and S. sobrinus in a disc diffusion method. Minimal inhibitory concentrations (MICs) of DF against these bacteria ranged from 0.25 to 0.5 mg/mL. Furthermore, DF significantly inhibited the caries-inducing factors of these bacteria. At sub-MIC levels, DF inhibited in vitro dental plaque formation by S. mutans and S. sobrinus (IC50= 0.079 and 0.142 mg/mL, respectively), which was caused, in part, by the inhibitory effect on the activity of glucosyltransferases. A significant reduction of glycolytic acid production was found at the concentration as low as 0.032 mg/mL for S. mutans and 0.063 mg/mL for S. sobrinus. The possible bioactive compounds that are inducing in vitro anti-cariogenic activity of DF are unknown. Based on the preliminary phytochemical analysis, the activity of DF may be related to the presence of anthraquinones, cardiac glycosides, coumarines, sterols/terpenes, and phenolics. These results indicate that DF is probably useful for the control of dental plaque formation and subsequent dental caries development.

Authors+Show Affiliations

Department of Preventive Dentistry, Chonbuk National University, Jeonju, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16833017

Citation

Song, Ju-Hee, et al. "In Vitro Anti-cariogenic Activity of Dichloromethane Fraction From Rheum Undulatum L. Root." Archives of Pharmacal Research, vol. 29, no. 6, 2006, pp. 490-6.
Song JH, Yang TC, Chang KW, et al. In vitro anti-cariogenic activity of dichloromethane fraction from Rheum undulatum L. root. Arch Pharm Res. 2006;29(6):490-6.
Song, J. H., Yang, T. C., Chang, K. W., Han, S. K., Yi, H. K., & Jeon, J. G. (2006). In vitro anti-cariogenic activity of dichloromethane fraction from Rheum undulatum L. root. Archives of Pharmacal Research, 29(6), pp. 490-6.
Song JH, et al. In Vitro Anti-cariogenic Activity of Dichloromethane Fraction From Rheum Undulatum L. Root. Arch Pharm Res. 2006;29(6):490-6. PubMed PMID: 16833017.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro anti-cariogenic activity of dichloromethane fraction from Rheum undulatum L. root. AU - Song,Ju-Hee, AU - Yang,Tae-Cheol, AU - Chang,Kee-Wan, AU - Han,Seong-Kyu, AU - Yi,Ho-Keun, AU - Jeon,Jae-Gyu, PY - 2006/7/13/pubmed PY - 2006/9/26/medline PY - 2006/7/13/entrez SP - 490 EP - 6 JF - Archives of pharmacal research JO - Arch. Pharm. Res. VL - 29 IS - 6 N2 - This study aimed to evaluate in vitro effects of Rheum undulatum L. root on the development of dental caries, especially its effects on viability, dental plaque formation, and glycolytic acid production of Streptococcus mutans and Streptococcus sobrinus. Methanol extract of Rheum undulatum L. root and its fractions were prepared and tested. Among the test extract and fractions, dichloromethane fraction (DF) showed the most active antibacterial activity (inhibition zone: 13-17 mm) against S. mutans and S. sobrinus in a disc diffusion method. Minimal inhibitory concentrations (MICs) of DF against these bacteria ranged from 0.25 to 0.5 mg/mL. Furthermore, DF significantly inhibited the caries-inducing factors of these bacteria. At sub-MIC levels, DF inhibited in vitro dental plaque formation by S. mutans and S. sobrinus (IC50= 0.079 and 0.142 mg/mL, respectively), which was caused, in part, by the inhibitory effect on the activity of glucosyltransferases. A significant reduction of glycolytic acid production was found at the concentration as low as 0.032 mg/mL for S. mutans and 0.063 mg/mL for S. sobrinus. The possible bioactive compounds that are inducing in vitro anti-cariogenic activity of DF are unknown. Based on the preliminary phytochemical analysis, the activity of DF may be related to the presence of anthraquinones, cardiac glycosides, coumarines, sterols/terpenes, and phenolics. These results indicate that DF is probably useful for the control of dental plaque formation and subsequent dental caries development. SN - 0253-6269 UR - https://www.unboundmedicine.com/medline/citation/16833017/In_vitro_anti_cariogenic_activity_of_dichloromethane_fraction_from_Rheum_undulatum_L__root_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=16833017.ui DB - PRIME DP - Unbound Medicine ER -