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Digestive enzymes during development of Ceratitis capitata (Diptera:Tephritidae) and effects of SBTI on its digestive serine proteinase targets.
Insect Biochem Mol Biol. 2006 Jul; 36(7):561-9.IB

Abstract

The digestive system of Ceratitis capitata was characterized during its larval development and in the insect stage. Disaccharidases against maltose and sucrose were more evident in the 2nd and 3rd day of larval development and in the adult stage, respectively. Glycosil-hydrolyses with higher specific alpha-galactosidasic and beta-galactosidasic activities were detected in the 2nd and 3rd day of the larval stage, respectively. Specific proteolytic activities against azocasein showed an increase in the 4th and 5th day of the larval stage and in the adult stage. Specific hemoglobin activities were constant between 2nd and 6th day of the larval stage. The larvae used mainly serine proteinases, such as trypsin/chymotrypsin, and the adult insects only chymotrypsin-like enzymes in their digestive process. Two serine proteinases were separated from zymogram between the 4th and 5th day of larval development and in the adult stage. Effect of soybean trypsin inhibitor (SBTI, a serine proteinase inhibitor) on development of C. capitata was examined by bioassay. C. capitata was susceptible to SBTI which affected larval mass at ED50 3.01%.

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Authors+Show Affiliations

Silva FC
Laboratório de Química e Função de Proteínas Bioativas, Departamento de Bioquímica, Centro de Biociências, Universidade Federal do Rio Grande do Norte, 59072-970 Natal, RN, Brazil.
Alcazar A
No affiliation info available
Macedo LL
No affiliation info available
Oliveira AS
No affiliation info available
Macedo FP
No affiliation info available
Abreu LR
No affiliation info available
Santos EA
No affiliation info available
Sales MP
No affiliation info available

MeSH

AnimalsCaseinsDipteraElectrophoresis, Polyacrylamide GelGlycoside HydrolasesSerine EndopeptidasesTrypsin Inhibitor, Kunitz Soybean

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16835021

Citation

Silva, Fátima C B L., et al. "Digestive Enzymes During Development of Ceratitis Capitata (Diptera:Tephritidae) and Effects of SBTI On Its Digestive Serine Proteinase Targets." Insect Biochemistry and Molecular Biology, vol. 36, no. 7, 2006, pp. 561-9.
Silva FC, Alcazar A, Macedo LL, et al. Digestive enzymes during development of Ceratitis capitata (Diptera:Tephritidae) and effects of SBTI on its digestive serine proteinase targets. Insect Biochem Mol Biol. 2006;36(7):561-9.
Silva, F. C., Alcazar, A., Macedo, L. L., Oliveira, A. S., Macedo, F. P., Abreu, L. R., Santos, E. A., & Sales, M. P. (2006). Digestive enzymes during development of Ceratitis capitata (Diptera:Tephritidae) and effects of SBTI on its digestive serine proteinase targets. Insect Biochemistry and Molecular Biology, 36(7), 561-9.
Silva FC, et al. Digestive Enzymes During Development of Ceratitis Capitata (Diptera:Tephritidae) and Effects of SBTI On Its Digestive Serine Proteinase Targets. Insect Biochem Mol Biol. 2006;36(7):561-9. PubMed PMID: 16835021.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Digestive enzymes during development of Ceratitis capitata (Diptera:Tephritidae) and effects of SBTI on its digestive serine proteinase targets. AU - Silva,Fátima C B L, AU - Alcazar,Alonso, AU - Macedo,Leonardo L P, AU - Oliveira,Adeliana S, AU - Macedo,Francisco P, AU - Abreu,Luiz R D, AU - Santos,Elizeu A, AU - Sales,Mauricio P, Y1 - 2006/05/05/ PY - 2006/02/23/received PY - 2006/04/15/revised PY - 2006/04/20/accepted PY - 2006/7/13/pubmed PY - 2006/9/21/medline PY - 2006/7/13/entrez SP - 561 EP - 9 JF - Insect biochemistry and molecular biology JO - Insect Biochem Mol Biol VL - 36 IS - 7 N2 - The digestive system of Ceratitis capitata was characterized during its larval development and in the insect stage. Disaccharidases against maltose and sucrose were more evident in the 2nd and 3rd day of larval development and in the adult stage, respectively. Glycosil-hydrolyses with higher specific alpha-galactosidasic and beta-galactosidasic activities were detected in the 2nd and 3rd day of the larval stage, respectively. Specific proteolytic activities against azocasein showed an increase in the 4th and 5th day of the larval stage and in the adult stage. Specific hemoglobin activities were constant between 2nd and 6th day of the larval stage. The larvae used mainly serine proteinases, such as trypsin/chymotrypsin, and the adult insects only chymotrypsin-like enzymes in their digestive process. Two serine proteinases were separated from zymogram between the 4th and 5th day of larval development and in the adult stage. Effect of soybean trypsin inhibitor (SBTI, a serine proteinase inhibitor) on development of C. capitata was examined by bioassay. C. capitata was susceptible to SBTI which affected larval mass at ED50 3.01%. SN - 0965-1748 UR - https://www.unboundmedicine.com/medline/citation/16835021/Digestive_enzymes_during_development_of_Ceratitis_capitata__Diptera:Tephritidae__and_effects_of_SBTI_on_its_digestive_serine_proteinase_targets_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0965-1748(06)00092-0 DB - PRIME DP - Unbound Medicine ER -
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