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Morphologic changes induced by oral long-term treatment with 8-prenylnaringenin in the uterus, vagina, and mammary gland of castrated rats.
Menopause. 2006 Jul-Aug; 13(4):669-77.M

Abstract

OBJECTIVE

The flavonoid 8-prenylnaringenin (8-PN) is found in hops, and hence in beer, and is also increasingly consumed as a food supplement. It is the strongest known phytoestrogen, which makes it a good candidate as an alternative to hormone therapy. Its putatively undesired estrogenic effects in the uterus and mammary gland have not yet been thoroughly investigated. Therefore, we performed a long-term oral administration experiment.

DESIGN

Rats were ovariectomized and fed for 3 months with soy-free chow containing estradiol (E(2)) or 8-PN, both in two doses (8-PN: 6.77 mg or 68.42 mg/kg body weight; E(2): 0.17 mg or 0.7 mg/kg body weight) or no additives. Analysis was mainly focused on morphologic and immunocytochemical parameters. Expression of proliferating cell nuclear antigen as a proliferation marker and of progesterone receptor was quantified in the mammary gland.

RESULTS

Uteri of animals treated with both E(2) doses and the high 8-PN dose had increased weight and showed histologic estrogen-induced features. 8-PN at the high dose induced epithelial polypoid formation unique to this group. Compared to the atrophic controls, both E(2) doses and the high 8-PN dose induced hyperplastic epithelia in the vagina. The high doses of E(2) and 8-PN caused secretion in the mammary gland, whereas proliferation and progesterone receptor expression were stimulated by both E(2) doses and the high 8-PN dose.

CONCLUSIONS

E(2) and 8-PN share many effects in the three studied organs, but some differences in the mechanism of action appear to exist.

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Authors+Show Affiliations

Rimoldi G
Department of Clinical and Experimental Endocrinology, University of Göttingen, Göttingen, Germany.
Christoffel J
No affiliation info available
Wuttke W
No affiliation info available

MeSH

Administration, OralAnimalsCell ProliferationDisease Models, AnimalDose-Response Relationship, DrugDrug Administration ScheduleEstrogen Replacement TherapyFemaleFlavanonesHumulusMammary Glands, AnimalMenopauseOvariectomyPhytoestrogensPhytotherapyRatsRats, Sprague-DawleyUterusVagina

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16837889

Citation

Rimoldi, Guillermo, et al. "Morphologic Changes Induced By Oral Long-term Treatment With 8-prenylnaringenin in the Uterus, Vagina, and Mammary Gland of Castrated Rats." Menopause (New York, N.Y.), vol. 13, no. 4, 2006, pp. 669-77.
Rimoldi G, Christoffel J, Wuttke W. Morphologic changes induced by oral long-term treatment with 8-prenylnaringenin in the uterus, vagina, and mammary gland of castrated rats. Menopause. 2006;13(4):669-77.
Rimoldi, G., Christoffel, J., & Wuttke, W. (2006). Morphologic changes induced by oral long-term treatment with 8-prenylnaringenin in the uterus, vagina, and mammary gland of castrated rats. Menopause (New York, N.Y.), 13(4), 669-77.
Rimoldi G, Christoffel J, Wuttke W. Morphologic Changes Induced By Oral Long-term Treatment With 8-prenylnaringenin in the Uterus, Vagina, and Mammary Gland of Castrated Rats. Menopause. 2006 Jul-Aug;13(4):669-77. PubMed PMID: 16837889.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Morphologic changes induced by oral long-term treatment with 8-prenylnaringenin in the uterus, vagina, and mammary gland of castrated rats. AU - Rimoldi,Guillermo, AU - Christoffel,Julie, AU - Wuttke,Wolfgang, PY - 2006/7/14/pubmed PY - 2006/10/20/medline PY - 2006/7/14/entrez SP - 669 EP - 77 JF - Menopause (New York, N.Y.) JO - Menopause VL - 13 IS - 4 N2 - OBJECTIVE: The flavonoid 8-prenylnaringenin (8-PN) is found in hops, and hence in beer, and is also increasingly consumed as a food supplement. It is the strongest known phytoestrogen, which makes it a good candidate as an alternative to hormone therapy. Its putatively undesired estrogenic effects in the uterus and mammary gland have not yet been thoroughly investigated. Therefore, we performed a long-term oral administration experiment. DESIGN: Rats were ovariectomized and fed for 3 months with soy-free chow containing estradiol (E(2)) or 8-PN, both in two doses (8-PN: 6.77 mg or 68.42 mg/kg body weight; E(2): 0.17 mg or 0.7 mg/kg body weight) or no additives. Analysis was mainly focused on morphologic and immunocytochemical parameters. Expression of proliferating cell nuclear antigen as a proliferation marker and of progesterone receptor was quantified in the mammary gland. RESULTS: Uteri of animals treated with both E(2) doses and the high 8-PN dose had increased weight and showed histologic estrogen-induced features. 8-PN at the high dose induced epithelial polypoid formation unique to this group. Compared to the atrophic controls, both E(2) doses and the high 8-PN dose induced hyperplastic epithelia in the vagina. The high doses of E(2) and 8-PN caused secretion in the mammary gland, whereas proliferation and progesterone receptor expression were stimulated by both E(2) doses and the high 8-PN dose. CONCLUSIONS: E(2) and 8-PN share many effects in the three studied organs, but some differences in the mechanism of action appear to exist. SN - 1072-3714 UR - https://www.unboundmedicine.com/medline/citation/16837889/Morphologic_changes_induced_by_oral_long_term_treatment_with_8_prenylnaringenin_in_the_uterus_vagina_and_mammary_gland_of_castrated_rats_ L2 - https://doi.org/10.1097/01.gme.0000196596.90076.d0 DB - PRIME DP - Unbound Medicine ER -