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The effects of fructo-oligosaccharides in combination with soy protein on bone in osteopenic ovariectomized rats.
Menopause 2006 Jul-Aug; 13(4):692-9M

Abstract

OBJECTIVE

The intestinal microflora is important in rendering soy isoflavones bioavailable by facilitating their conversion to equol. Hence, substances that can modulate the intestinal microflora could affect the bioavailability of isoflavones. In this study, we examined the effects of fructo-oligosaccharides (FOS), a prebiotic, on enhancing the effects of soy isoflavones on bone in ovariectomized osteopenic female rats.

DESIGN

Sixty-three 9-month-old female Sprague-Dawley rats were either sham-operated (Sham; one group) or ovariectomized (Ovx; four groups) and were fed a control diet for 3 months to induce bone loss. After bone loss was confirmed via dual-energy x-ray absorptiometry, rats were placed on dietary treatment for 4 months. The Sham and one Ovx group received a control diet, and the remaining Ovx groups received either a soy protein-based diet (Soy), a FOS-supplemented diet (FOS), or a soy protein-based and FOS-supplemented diet (Soy+FOS). Before the termination of the study, whole-body bone mineral density (BMD) and bone mineral content (BMC) were assessed under anesthesia. Immediately after euthanasia, bone specimens were collected for the assessments of BMD, BMC, and biomechanical and microarchitectural properties.

RESULTS

Whole-body BMD values were significantly higher in FOS and Soy+FOS groups compared with Ovx controls. The tibial BMC increased by 10%, 6%, and 4% in Soy, FOS, and Soy+FOS groups, respectively, compared to the Ovx control group. FOS and FOS+Soy treatments had the most pronounced effects in enhancing lumbar BMC and BMD. The FOS+Soy combination effectively improved tibial microarchitectural properties by enhancing trabecular number and lowering trabecular separation compared with Ovx controls. The effects of dietary treatments on lumbar microarchitectural properties were minimal and biomechanical properties of the femur were not affected by any of the dietary treatments.

CONCLUSION

Our findings suggest that, although incorporation of either soy or FOS in the diet of Ovx rats can improve BMD of the whole body, tibiae, and lumbar vertebrae, their combination had no any additive effects. However, in terms of microarchitecture, the combination of soy and FOS had a greater effect in reversing the loss of certain microarchitectural parameters such as tibial trabecular number, separation, and thickness.

Authors+Show Affiliations

Department of Nutrition, Food and Exercise Sciences, Florida State University, Tallahassee, FL 32306, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

16837891

Citation

Devareddy, Latha, et al. "The Effects of Fructo-oligosaccharides in Combination With Soy Protein On Bone in Osteopenic Ovariectomized Rats." Menopause (New York, N.Y.), vol. 13, no. 4, 2006, pp. 692-9.
Devareddy L, Khalil DA, Korlagunta K, et al. The effects of fructo-oligosaccharides in combination with soy protein on bone in osteopenic ovariectomized rats. Menopause. 2006;13(4):692-9.
Devareddy, L., Khalil, D. A., Korlagunta, K., Hooshmand, S., Bellmer, D. D., & Arjmandi, B. H. (2006). The effects of fructo-oligosaccharides in combination with soy protein on bone in osteopenic ovariectomized rats. Menopause (New York, N.Y.), 13(4), pp. 692-9.
Devareddy L, et al. The Effects of Fructo-oligosaccharides in Combination With Soy Protein On Bone in Osteopenic Ovariectomized Rats. Menopause. 2006;13(4):692-9. PubMed PMID: 16837891.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effects of fructo-oligosaccharides in combination with soy protein on bone in osteopenic ovariectomized rats. AU - Devareddy,Latha, AU - Khalil,Dania A, AU - Korlagunta,Kiranmayi, AU - Hooshmand,Shirin, AU - Bellmer,Danielle D, AU - Arjmandi,Bahram H, PY - 2006/7/14/pubmed PY - 2006/10/20/medline PY - 2006/7/14/entrez SP - 692 EP - 9 JF - Menopause (New York, N.Y.) JO - Menopause VL - 13 IS - 4 N2 - OBJECTIVE: The intestinal microflora is important in rendering soy isoflavones bioavailable by facilitating their conversion to equol. Hence, substances that can modulate the intestinal microflora could affect the bioavailability of isoflavones. In this study, we examined the effects of fructo-oligosaccharides (FOS), a prebiotic, on enhancing the effects of soy isoflavones on bone in ovariectomized osteopenic female rats. DESIGN: Sixty-three 9-month-old female Sprague-Dawley rats were either sham-operated (Sham; one group) or ovariectomized (Ovx; four groups) and were fed a control diet for 3 months to induce bone loss. After bone loss was confirmed via dual-energy x-ray absorptiometry, rats were placed on dietary treatment for 4 months. The Sham and one Ovx group received a control diet, and the remaining Ovx groups received either a soy protein-based diet (Soy), a FOS-supplemented diet (FOS), or a soy protein-based and FOS-supplemented diet (Soy+FOS). Before the termination of the study, whole-body bone mineral density (BMD) and bone mineral content (BMC) were assessed under anesthesia. Immediately after euthanasia, bone specimens were collected for the assessments of BMD, BMC, and biomechanical and microarchitectural properties. RESULTS: Whole-body BMD values were significantly higher in FOS and Soy+FOS groups compared with Ovx controls. The tibial BMC increased by 10%, 6%, and 4% in Soy, FOS, and Soy+FOS groups, respectively, compared to the Ovx control group. FOS and FOS+Soy treatments had the most pronounced effects in enhancing lumbar BMC and BMD. The FOS+Soy combination effectively improved tibial microarchitectural properties by enhancing trabecular number and lowering trabecular separation compared with Ovx controls. The effects of dietary treatments on lumbar microarchitectural properties were minimal and biomechanical properties of the femur were not affected by any of the dietary treatments. CONCLUSION: Our findings suggest that, although incorporation of either soy or FOS in the diet of Ovx rats can improve BMD of the whole body, tibiae, and lumbar vertebrae, their combination had no any additive effects. However, in terms of microarchitecture, the combination of soy and FOS had a greater effect in reversing the loss of certain microarchitectural parameters such as tibial trabecular number, separation, and thickness. SN - 1072-3714 UR - https://www.unboundmedicine.com/medline/citation/16837891/The_effects_of_fructo_oligosaccharides_in_combination_with_soy_protein_on_bone_in_osteopenic_ovariectomized_rats_ L2 - http://Insights.ovid.com/pubmed?pmid=16837891 DB - PRIME DP - Unbound Medicine ER -