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Distinct ASIC currents are expressed in rat putative nociceptors and are modulated by nerve injury.
J Physiol. 2006 Oct 01; 576(Pt 1):215-34.JP

Abstract

The H(+)-gated acid-sensing ion channels (ASICs) are expressed in dorsal root ganglion (DRG) neurones. Studies with ASIC knockout mice indicated either a pro-nociceptive or a modulatory role of ASICs in pain sensation. We have investigated in freshly isolated rat DRG neurones whether neurones with different ASIC current properties exist, which may explain distinct cellular roles, and we have investigated ASIC regulation in an experimental model of neuropathic pain. Small-diameter DRG neurones expressed three different ASIC current types which were all preferentially expressed in putative nociceptors. Type 1 currents were mediated by ASIC1a homomultimers and characterized by steep pH dependence of current activation in the pH range 6.8-6.0. Type 3 currents were activated in a similar pH range as type 1, while type 2 currents were activated at pH < 6. When activated by acidification to pH 6.8 or 6.5, the probability of inducing action potentials correlated with the ASIC current density. Nerve injury induced differential regulation of ASIC subunit expression and selective changes in ASIC function in DRG neurones, suggesting a complex reorganization of ASICs during the development of neuropathic pain. In summary, we describe a basis for distinct cellular functions of different ASIC types in small-diameter DRG neurones.

Authors+Show Affiliations

Département de Pharmacologie et de Toxicologie, Université de Lausanne Rue du Bugnon 27, CH-1005 Lausanne, Switzerland.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16840516

Citation

Poirot, Olivier, et al. "Distinct ASIC Currents Are Expressed in Rat Putative Nociceptors and Are Modulated By Nerve Injury." The Journal of Physiology, vol. 576, no. Pt 1, 2006, pp. 215-34.
Poirot O, Berta T, Decosterd I, et al. Distinct ASIC currents are expressed in rat putative nociceptors and are modulated by nerve injury. J Physiol. 2006;576(Pt 1):215-34.
Poirot, O., Berta, T., Decosterd, I., & Kellenberger, S. (2006). Distinct ASIC currents are expressed in rat putative nociceptors and are modulated by nerve injury. The Journal of Physiology, 576(Pt 1), 215-34.
Poirot O, et al. Distinct ASIC Currents Are Expressed in Rat Putative Nociceptors and Are Modulated By Nerve Injury. J Physiol. 2006 Oct 1;576(Pt 1):215-34. PubMed PMID: 16840516.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Distinct ASIC currents are expressed in rat putative nociceptors and are modulated by nerve injury. AU - Poirot,Olivier, AU - Berta,Temugin, AU - Decosterd,Isabelle, AU - Kellenberger,Stephan, Y1 - 2006/07/13/ PY - 2006/7/15/pubmed PY - 2006/12/9/medline PY - 2006/7/15/entrez SP - 215 EP - 34 JF - The Journal of physiology JO - J Physiol VL - 576 IS - Pt 1 N2 - The H(+)-gated acid-sensing ion channels (ASICs) are expressed in dorsal root ganglion (DRG) neurones. Studies with ASIC knockout mice indicated either a pro-nociceptive or a modulatory role of ASICs in pain sensation. We have investigated in freshly isolated rat DRG neurones whether neurones with different ASIC current properties exist, which may explain distinct cellular roles, and we have investigated ASIC regulation in an experimental model of neuropathic pain. Small-diameter DRG neurones expressed three different ASIC current types which were all preferentially expressed in putative nociceptors. Type 1 currents were mediated by ASIC1a homomultimers and characterized by steep pH dependence of current activation in the pH range 6.8-6.0. Type 3 currents were activated in a similar pH range as type 1, while type 2 currents were activated at pH < 6. When activated by acidification to pH 6.8 or 6.5, the probability of inducing action potentials correlated with the ASIC current density. Nerve injury induced differential regulation of ASIC subunit expression and selective changes in ASIC function in DRG neurones, suggesting a complex reorganization of ASICs during the development of neuropathic pain. In summary, we describe a basis for distinct cellular functions of different ASIC types in small-diameter DRG neurones. SN - 0022-3751 UR - https://www.unboundmedicine.com/medline/citation/16840516/Distinct_ASIC_currents_are_expressed_in_rat_putative_nociceptors_and_are_modulated_by_nerve_injury_ L2 - https://doi.org/10.1113/jphysiol.2006.113035 DB - PRIME DP - Unbound Medicine ER -