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Immunomodulatory effects of polysaccharides produced by Lactobacillus delbrueckii ssp. bulgaricus OLL1073R-1.
J Dairy Sci. 2006 Aug; 89(8):2873-81.JD

Abstract

The extracellular polysaccharides (EPS) produced by lactic acid bacteria (LAB) are associated with the rheology, texture, and mouthfeel of fermented milk products, including yogurt. This study investigated the immunomodulatory effects of EPS purified from the culture supernatant of Lactobacillus delbrueckii ssp. bulgaricus (L. bulgaricus) OLL1073R-1. The crude EPS were prepared from the culture supernatant of L. bulgaricus OLL1073R-1 by standard chromatographic methods, and were fractionated into neutral EPS and acidic EPS (APS). Acidic EPS were further fractionated into high molecular weight APS (H-APS) and low molecular weight APS (L-APS). High molecular weight APS were shown to be phosphopolysaccharides containing D-glucose, D-galactose, and phosphorus. Stimulation of mouse splenocytes by H-APS significantly increased interferon-gamma production, and, moreover, orally administered H-APS augmented natural killer cell activity. Oral administration of yogurt fermented with L. bulgaricus OLL1073R-1 and Streptococcus thermophilus OLS3059 to mice showed a similar level of immunomodulation as H-APS. However, these effects were not detected following administration of yogurt fermented with the starter combination of L. bulgaricus OLL1256 and S. thermophilus OLS3295. We conclude from these findings that yogurt fermented with L. bulgaricus OLL1073R-1, containing immunostimulative EPS, would have an immunomodulatory effect on the human body.

Links

Publisher Full Text
linkinghub.elsevier.com
linkinghub.elsevier.com

Authors+Show Affiliations

Makino S
Food Science Institute, Meiji Dairies Corp., 540 Naruda, Odawara, Kanagawa 250-0862, Japan. seiya_makino@meiji-milk.com
Ikegami S
No affiliation info available
Kano H
No affiliation info available
Sashihara T
No affiliation info available
Sugano H
No affiliation info available
Horiuchi H
No affiliation info available
Saito T
No affiliation info available
Oda M
No affiliation info available

MeSH

AnimalsCulture Media, ConditionedFermentationGalactoseGlucoseImmunologic FactorsInterferon-gammaInterleukin-4Killer Cells, NaturalLactobacillus delbrueckiiMaleMiceMice, Inbred BALB CMice, Inbred C3HMolecular WeightPhosphorusPolysaccharides, BacterialSpleenStreptococcus thermophilusYogurt

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16840603

Citation

Makino, S, et al. "Immunomodulatory Effects of Polysaccharides Produced By Lactobacillus Delbrueckii Ssp. Bulgaricus OLL1073R-1." Journal of Dairy Science, vol. 89, no. 8, 2006, pp. 2873-81.
Makino S, Ikegami S, Kano H, et al. Immunomodulatory effects of polysaccharides produced by Lactobacillus delbrueckii ssp. bulgaricus OLL1073R-1. J Dairy Sci. 2006;89(8):2873-81.
Makino, S., Ikegami, S., Kano, H., Sashihara, T., Sugano, H., Horiuchi, H., Saito, T., & Oda, M. (2006). Immunomodulatory effects of polysaccharides produced by Lactobacillus delbrueckii ssp. bulgaricus OLL1073R-1. Journal of Dairy Science, 89(8), 2873-81.
Makino S, et al. Immunomodulatory Effects of Polysaccharides Produced By Lactobacillus Delbrueckii Ssp. Bulgaricus OLL1073R-1. J Dairy Sci. 2006;89(8):2873-81. PubMed PMID: 16840603.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunomodulatory effects of polysaccharides produced by Lactobacillus delbrueckii ssp. bulgaricus OLL1073R-1. AU - Makino,S, AU - Ikegami,S, AU - Kano,H, AU - Sashihara,T, AU - Sugano,H, AU - Horiuchi,H, AU - Saito,T, AU - Oda,M, PY - 2006/7/15/pubmed PY - 2006/10/14/medline PY - 2006/7/15/entrez SP - 2873 EP - 81 JF - Journal of dairy science JO - J Dairy Sci VL - 89 IS - 8 N2 - The extracellular polysaccharides (EPS) produced by lactic acid bacteria (LAB) are associated with the rheology, texture, and mouthfeel of fermented milk products, including yogurt. This study investigated the immunomodulatory effects of EPS purified from the culture supernatant of Lactobacillus delbrueckii ssp. bulgaricus (L. bulgaricus) OLL1073R-1. The crude EPS were prepared from the culture supernatant of L. bulgaricus OLL1073R-1 by standard chromatographic methods, and were fractionated into neutral EPS and acidic EPS (APS). Acidic EPS were further fractionated into high molecular weight APS (H-APS) and low molecular weight APS (L-APS). High molecular weight APS were shown to be phosphopolysaccharides containing D-glucose, D-galactose, and phosphorus. Stimulation of mouse splenocytes by H-APS significantly increased interferon-gamma production, and, moreover, orally administered H-APS augmented natural killer cell activity. Oral administration of yogurt fermented with L. bulgaricus OLL1073R-1 and Streptococcus thermophilus OLS3059 to mice showed a similar level of immunomodulation as H-APS. However, these effects were not detected following administration of yogurt fermented with the starter combination of L. bulgaricus OLL1256 and S. thermophilus OLS3295. We conclude from these findings that yogurt fermented with L. bulgaricus OLL1073R-1, containing immunostimulative EPS, would have an immunomodulatory effect on the human body. SN - 1525-3198 UR - https://www.unboundmedicine.com/medline/citation/16840603/Immunomodulatory_effects_of_polysaccharides_produced_by_Lactobacillus_delbrueckii_ssp__bulgaricus_OLL1073R_1_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-0302(06)72560-7 DB - PRIME DP - Unbound Medicine ER -