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COX-1 inhibition enhances scratching behaviour in NC/Nga mice with atopic dermatitis.
Exp Dermatol. 2006 Aug; 15(8):582-8.ED

Abstract

NC/Nga (NC) mice, spontaneously develop an eczematous atopic dermatitis (AD)-like skin lesion when kept under conventional condition (Conv), but not under specific pathogen-free (SPF) conditions, have been thought to be an animal model of AD. We have previously shown that PGD(2) and arachidonic acid inhibited the scratching behaviour of NC mice, while indomethacin enhanced it. This study was designed to assess the role of cyclooxygenase (COX)-1 and COX-2 in the itch-related scratching behaviour of NC mice. We examined the expression of COX in the skin using real-time PCR and Western blotting and the effects of SC-560 (a COX-1 selective inhibitor) or NS-398 (a COX-2 selective inhibitor) on scratching behaviour in relation to skin prostaglandin (PG) levels in NC mice. COX-1 mRNA expression was unchanged and protein expression decreased in Conv NC mice compared with that of SPF mice. By contrast, COX-2 mRNA and protein expression increased in Conv NC mice. SC-560 increased scratching behaviour and significantly reduced skin PGD(2), PGE(2) and PGF(2alpha) levels, but NS-398 did not have effects on scratching and skin PG level. Moreover, the topical application of PGD(2), which might be the endogenous inhibitor of itching, suppressed the SC-560-induced enhancement of scratching behaviour by NC mice. These results suggest COX-1-coupled skin PGD(2) biosynthesis plays a physiological role in inhibiting regulation of pruritus in NC mice with AD.

Authors+Show Affiliations

Department of Pharmacology, Medicinal Research Laboratories, Taisho Pharmaceutical Co., Ltd, Kita-ku, Saitama, Japan. masanori.sugimoto@po.rd.taisho.co.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16842596

Citation

Sugimoto, Masanori, et al. "COX-1 Inhibition Enhances Scratching Behaviour in NC/Nga Mice With Atopic Dermatitis." Experimental Dermatology, vol. 15, no. 8, 2006, pp. 582-8.
Sugimoto M, Arai I, Futaki N, et al. COX-1 inhibition enhances scratching behaviour in NC/Nga mice with atopic dermatitis. Exp Dermatol. 2006;15(8):582-8.
Sugimoto, M., Arai, I., Futaki, N., Hashimoto, Y., Honma, Y., & Nakaike, S. (2006). COX-1 inhibition enhances scratching behaviour in NC/Nga mice with atopic dermatitis. Experimental Dermatology, 15(8), 582-8.
Sugimoto M, et al. COX-1 Inhibition Enhances Scratching Behaviour in NC/Nga Mice With Atopic Dermatitis. Exp Dermatol. 2006;15(8):582-8. PubMed PMID: 16842596.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - COX-1 inhibition enhances scratching behaviour in NC/Nga mice with atopic dermatitis. AU - Sugimoto,Masanori, AU - Arai,Iwao, AU - Futaki,Nobuko, AU - Hashimoto,Yuki, AU - Honma,Yusuke, AU - Nakaike,Shiro, PY - 2006/7/18/pubmed PY - 2007/1/4/medline PY - 2006/7/18/entrez SP - 582 EP - 8 JF - Experimental dermatology JO - Exp Dermatol VL - 15 IS - 8 N2 - NC/Nga (NC) mice, spontaneously develop an eczematous atopic dermatitis (AD)-like skin lesion when kept under conventional condition (Conv), but not under specific pathogen-free (SPF) conditions, have been thought to be an animal model of AD. We have previously shown that PGD(2) and arachidonic acid inhibited the scratching behaviour of NC mice, while indomethacin enhanced it. This study was designed to assess the role of cyclooxygenase (COX)-1 and COX-2 in the itch-related scratching behaviour of NC mice. We examined the expression of COX in the skin using real-time PCR and Western blotting and the effects of SC-560 (a COX-1 selective inhibitor) or NS-398 (a COX-2 selective inhibitor) on scratching behaviour in relation to skin prostaglandin (PG) levels in NC mice. COX-1 mRNA expression was unchanged and protein expression decreased in Conv NC mice compared with that of SPF mice. By contrast, COX-2 mRNA and protein expression increased in Conv NC mice. SC-560 increased scratching behaviour and significantly reduced skin PGD(2), PGE(2) and PGF(2alpha) levels, but NS-398 did not have effects on scratching and skin PG level. Moreover, the topical application of PGD(2), which might be the endogenous inhibitor of itching, suppressed the SC-560-induced enhancement of scratching behaviour by NC mice. These results suggest COX-1-coupled skin PGD(2) biosynthesis plays a physiological role in inhibiting regulation of pruritus in NC mice with AD. SN - 0906-6705 UR - https://www.unboundmedicine.com/medline/citation/16842596/COX_1_inhibition_enhances_scratching_behaviour_in_NC/Nga_mice_with_atopic_dermatitis_ L2 - https://doi.org/10.1111/j.1600-0625.2006.00447.x DB - PRIME DP - Unbound Medicine ER -