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Insoluble matrix components of glioma cells suppress LPS-mediated iNOS/NO induction in microglia.

Abstract

This study examines the influence of insoluble matrix components of glioma (astrocytoma) cells on LPS-mediated inducible nitric oxide (NO)/NO synthase (iNOS) induction in microglia cells. Insoluble matrix components prepared from C6 rat glioma cells strongly suppressed iNOS induction and subsequent NO release induced by LPS. Matrices prepared from several glioma cell lines displayed similar inhibitory effects on LPS-induced NO/iNOS induction, whereas matrices from primary cultured rat astrocytes had a minimal influence. Of the various purified ECM materials examined, collagen suppressed LPS-mediated iNOS/NO induction in microglia. C6 matrices potentiated LPS-induced NF-kappaB DNA binding/transcriptional activity, suggesting that the suppression of LPS-induced iNOS by C6 matrices is NF-kappaB independent. C6 matrices inhibited LPS-mediated activation of p38 and JNK MAP kinases. This study shows that non-diffusible factors derived from astrocytoma cells in the brain are critically involved in the suppression of microglial cell activation. Our results indicate that activation of microglia can be regulated by various cellular and pathological environmental conditions, not only through cell-cell contact or soluble factors, but also via insoluble matrix components.

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  • Authors+Show Affiliations

    ,

    Department of Physiology and Biophysics, Inha University, College of Medicine, Incheon, Republic of Korea.

    ,

    Source

    MeSH

    Animals
    Cell Death
    Cell Extracts
    Cells, Cultured
    Extracellular Matrix
    Glioma
    Inflammation Mediators
    JNK Mitogen-Activated Protein Kinases
    Lipopolysaccharides
    Mice
    Microglia
    NF-kappa B
    Nitric Oxide
    Nitric Oxide Synthase Type II
    Phosphorylation
    Rats
    Solubility
    p38 Mitogen-Activated Protein Kinases

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    16843440

    Citation

    Kim, Yoon-Jung, et al. "Insoluble Matrix Components of Glioma Cells Suppress LPS-mediated iNOS/NO Induction in Microglia." Biochemical and Biophysical Research Communications, vol. 347, no. 3, 2006, pp. 731-8.
    Kim YJ, Hwang SY, Han IO. Insoluble matrix components of glioma cells suppress LPS-mediated iNOS/NO induction in microglia. Biochem Biophys Res Commun. 2006;347(3):731-8.
    Kim, Y. J., Hwang, S. Y., & Han, I. O. (2006). Insoluble matrix components of glioma cells suppress LPS-mediated iNOS/NO induction in microglia. Biochemical and Biophysical Research Communications, 347(3), pp. 731-8.
    Kim YJ, Hwang SY, Han IO. Insoluble Matrix Components of Glioma Cells Suppress LPS-mediated iNOS/NO Induction in Microglia. Biochem Biophys Res Commun. 2006 Sep 1;347(3):731-8. PubMed PMID: 16843440.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Insoluble matrix components of glioma cells suppress LPS-mediated iNOS/NO induction in microglia. AU - Kim,Yoon-Jung, AU - Hwang,So-Young, AU - Han,Inn-Oc, Y1 - 2006/07/05/ PY - 2006/06/20/received PY - 2006/06/25/accepted PY - 2006/7/18/pubmed PY - 2006/9/14/medline PY - 2006/7/18/entrez SP - 731 EP - 8 JF - Biochemical and biophysical research communications JO - Biochem. Biophys. Res. Commun. VL - 347 IS - 3 N2 - This study examines the influence of insoluble matrix components of glioma (astrocytoma) cells on LPS-mediated inducible nitric oxide (NO)/NO synthase (iNOS) induction in microglia cells. Insoluble matrix components prepared from C6 rat glioma cells strongly suppressed iNOS induction and subsequent NO release induced by LPS. Matrices prepared from several glioma cell lines displayed similar inhibitory effects on LPS-induced NO/iNOS induction, whereas matrices from primary cultured rat astrocytes had a minimal influence. Of the various purified ECM materials examined, collagen suppressed LPS-mediated iNOS/NO induction in microglia. C6 matrices potentiated LPS-induced NF-kappaB DNA binding/transcriptional activity, suggesting that the suppression of LPS-induced iNOS by C6 matrices is NF-kappaB independent. C6 matrices inhibited LPS-mediated activation of p38 and JNK MAP kinases. This study shows that non-diffusible factors derived from astrocytoma cells in the brain are critically involved in the suppression of microglial cell activation. Our results indicate that activation of microglia can be regulated by various cellular and pathological environmental conditions, not only through cell-cell contact or soluble factors, but also via insoluble matrix components. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/16843440/Insoluble_matrix_components_of_glioma_cells_suppress_LPS_mediated_iNOS/NO_induction_in_microglia_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(06)01466-5 DB - PRIME DP - Unbound Medicine ER -