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In whole blood, LPS, TNF-alpha and GM-CSF increase monocyte uptake of 99mtechnetium stannous colloid but do not affect neutrophil uptake.
Nucl Med Biol. 2006 Jul; 33(5):645-51.NM

Abstract

INTRODUCTION

(99m)Technetium stannous colloid (TcSnC) is used in white cell scanning. It labels neutrophils and monocytes via phagocytosis, with uptake mediated by the phagocytic receptor CD11b/CD18 in neutrophils. Uptake of TcSnC is altered by gram-negative infection, possibly due to the endotoxin component lipopolysaccharide (LPS) or to cytokines released during infection (e.g., TNF-alpha and IFN-gamma). Endotoxemia and increased TNF-alpha levels also occur in inflammatory bowel disease. Another potential confounder in cell labeling is that sepsis patients may be treated with GM-CSF and G-CSF, which alter phagocytic cell function. This study aimed to determine how these factors affect TcSnC cellular uptake.

METHODS

Whole blood from six healthy volunteers was incubated with LPS, TNF-alpha, IFN-gamma, GM-CSF or G-CSF. Samples were then mixed with TcSnC. Blood was separated across density gradients and imaged using a gamma camera. Three radioactive count peaks were observed in each tube: free plasma activity, mononuclear cell uptake and neutrophil uptake.

RESULTS

Compared with controls, significant increases in mononuclear cell uptake were induced by LPS, TNF-alpha and GM-CSF stimulation. It was incidentally noted that exogenous estrogens appear to affect TcSnC labeling and may influence the neutrophil response to stimulation. Neutrophil uptake and plasma activity were not significantly affected. IFN-gamma and G-CSF had no significant effect.

CONCLUSIONS

In whole blood, the effect of LPS on TcSnC monocyte uptake is different to its effect on neutrophils, consistent with previously reported differences in CD11b/CD18 expression. TNF-alpha response parallels LPS response. GM-CSF also increases TcSnC uptake by monocytes. These effects should be considered when using TcSnC for imaging purposes, as they will tend to increase monocyte labeling. Estrogens may also affect TcSnC labeling. Responses to IFN-gamma and G-CSF are consistent with previously reported effects of these cytokines on CD11b/CD18 expression.

Authors+Show Affiliations

Townsville Nuclear Medicine, Mater Hospital, Pimlico, Queensland 4812, Australia. stuart.ramsay1@jcu.edu.auNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16843839

Citation

Ramsay, Stuart C., et al. "In Whole Blood, LPS, TNF-alpha and GM-CSF Increase Monocyte Uptake of 99mtechnetium Stannous Colloid but Do Not Affect Neutrophil Uptake." Nuclear Medicine and Biology, vol. 33, no. 5, 2006, pp. 645-51.
Ramsay SC, Maggs J, Powell K, et al. In whole blood, LPS, TNF-alpha and GM-CSF increase monocyte uptake of 99mtechnetium stannous colloid but do not affect neutrophil uptake. Nucl Med Biol. 2006;33(5):645-51.
Ramsay, S. C., Maggs, J., Powell, K., Barnes, J., & Ketheesan, N. (2006). In whole blood, LPS, TNF-alpha and GM-CSF increase monocyte uptake of 99mtechnetium stannous colloid but do not affect neutrophil uptake. Nuclear Medicine and Biology, 33(5), 645-51.
Ramsay SC, et al. In Whole Blood, LPS, TNF-alpha and GM-CSF Increase Monocyte Uptake of 99mtechnetium Stannous Colloid but Do Not Affect Neutrophil Uptake. Nucl Med Biol. 2006;33(5):645-51. PubMed PMID: 16843839.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In whole blood, LPS, TNF-alpha and GM-CSF increase monocyte uptake of 99mtechnetium stannous colloid but do not affect neutrophil uptake. AU - Ramsay,Stuart C, AU - Maggs,Jacqueline, AU - Powell,Kellie, AU - Barnes,Jodie, AU - Ketheesan,Natkunam, PY - 2006/03/14/received PY - 2006/04/04/revised PY - 2006/04/15/accepted PY - 2006/7/18/pubmed PY - 2006/10/7/medline PY - 2006/7/18/entrez SP - 645 EP - 51 JF - Nuclear medicine and biology JO - Nucl Med Biol VL - 33 IS - 5 N2 - INTRODUCTION: (99m)Technetium stannous colloid (TcSnC) is used in white cell scanning. It labels neutrophils and monocytes via phagocytosis, with uptake mediated by the phagocytic receptor CD11b/CD18 in neutrophils. Uptake of TcSnC is altered by gram-negative infection, possibly due to the endotoxin component lipopolysaccharide (LPS) or to cytokines released during infection (e.g., TNF-alpha and IFN-gamma). Endotoxemia and increased TNF-alpha levels also occur in inflammatory bowel disease. Another potential confounder in cell labeling is that sepsis patients may be treated with GM-CSF and G-CSF, which alter phagocytic cell function. This study aimed to determine how these factors affect TcSnC cellular uptake. METHODS: Whole blood from six healthy volunteers was incubated with LPS, TNF-alpha, IFN-gamma, GM-CSF or G-CSF. Samples were then mixed with TcSnC. Blood was separated across density gradients and imaged using a gamma camera. Three radioactive count peaks were observed in each tube: free plasma activity, mononuclear cell uptake and neutrophil uptake. RESULTS: Compared with controls, significant increases in mononuclear cell uptake were induced by LPS, TNF-alpha and GM-CSF stimulation. It was incidentally noted that exogenous estrogens appear to affect TcSnC labeling and may influence the neutrophil response to stimulation. Neutrophil uptake and plasma activity were not significantly affected. IFN-gamma and G-CSF had no significant effect. CONCLUSIONS: In whole blood, the effect of LPS on TcSnC monocyte uptake is different to its effect on neutrophils, consistent with previously reported differences in CD11b/CD18 expression. TNF-alpha response parallels LPS response. GM-CSF also increases TcSnC uptake by monocytes. These effects should be considered when using TcSnC for imaging purposes, as they will tend to increase monocyte labeling. Estrogens may also affect TcSnC labeling. Responses to IFN-gamma and G-CSF are consistent with previously reported effects of these cytokines on CD11b/CD18 expression. SN - 0969-8051 UR - https://www.unboundmedicine.com/medline/citation/16843839/In_whole_blood_LPS_TNF_alpha_and_GM_CSF_increase_monocyte_uptake_of_99mtechnetium_stannous_colloid_but_do_not_affect_neutrophil_uptake_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0969-8051(06)00068-0 DB - PRIME DP - Unbound Medicine ER -